Salicylic acid regulates polyamine biosynthesis during drought responses in oat

Canales, Francisco José; Montilla-Bascón, Gracia; Rispail, Nicolás; Prats, Elena

doi:10.1080/15592324.2019.1651183

Cited by 32 publications

(17 citation statements)

References 20 publications

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“…Recent studies have tackled the drought tolerance response of oat. These covered different aspects focusing on adaptation and yield potential (Sánchez-Martín et al, 2014; Sadras et al, 2017; Sánchez-Martín et al, 2018), on the role of different metabolites during drought tolerance responses (Sánchez-Martín et al, 2015; Sánchez-Martín et al, 2017; Gao et al, 2018; Canales et al, 2019), or on the identification of physiological and biochemical markers to select drought tolerant genotypes (Rabiei et al, 2012; Sánchez-Martín et al, 2012; Marcinska et al, 2017; Rispail et al, 2018). However, and following the same trend as for other crops, very few information are available regarding root responses engaged for drought tolerance in oat.…”

Section: Introductionmentioning

confidence: 99%

Deciphering Root Architectural Traits Involved to Cope With Water Deficit in Oat

et al. 2019

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Drought tolerance is a complex phenomenon comprising many physiological, biochemical and morphological changes at both aerial and below ground levels. We aim to reveal changes on root morphology that promote drought tolerance in oat in both seedling and adult plants. To this aim, we employed two oat genotypes, previously characterized as susceptible and tolerant to drought. Root phenotyping was carried out on young plants grown either in pots or in rhizotrons under controlled environments, and on adult plants grown in big containers under field conditions. Overall, the tolerant genotype showed an increased root length, branching rate, root surface, and length of fine roots, while coarse to fine ratio decreased as compared with the susceptible genotype. We also observed a high and significant correlation between various morphological root traits within and between experiments, identifying several of them as appropriate markers to identify drought tolerant oat genotypes. Stimulation of fine root growth was one of the most prominent responses to cope with gradual soil water depletion, in both seedlings and adult plants. Although seedling experiments did not exactly match the response of adult plants, they were similarly informative for discriminating between tolerant and susceptible genotypes. This might contribute to easier and faster phenotyping of large amount of plants.

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Section: Introductionmentioning

confidence: 99%

Deciphering Root Architectural Traits Involved to Cope With Water Deficit in Oat

et al. 2019

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“…In these distant sites, MeSA is de-methylated back to SA, which then contributes to establishing a systemic immunity (systemic acquired resistance, SAR) [ 3 , 4 ]. SA is also a part of some abiotic stress reactions in plants, e.g., drought responses in oat [ 5 ] or salinity tolerance in Arabidopsis thaliana [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Binding of Salicylic Acid to Arabidopsis thaliana Chloroplastic GAPDH-A1

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Hellal

Bouceba

et al. 2020

IJMS

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Salicylic acid (SA) has an essential role in the responses of plants to pathogens. SA initiates defence signalling via binding to proteins. NPR1 is a transcriptional co-activator and a key target of SA binding. Many other proteins have recently been shown to bind SA. Amongst these proteins are important enzymes of primary metabolism. This fact could stand behind SA’s ability to control energy fluxes in stressed plants. Nevertheless, only sparse information exists on the role and mechanisms of such binding. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was previously demonstrated to bind SA both in human and plants. Here, we detail that the A1 isomer of chloroplastic glyceraldehyde 3-phosphate dehydrogenase (GAPA1) from Arabidopsis thaliana binds SA with a KD of 16.7 nM, as shown in surface plasmon resonance experiments. Besides, we show that SA inhibits its GAPDH activity in vitro. To gain some insight into the underlying molecular interactions and binding mechanism, we combined in silico molecular docking experiments and molecular dynamics simulations on the free protein and protein–ligand complex. The molecular docking analysis yielded to the identification of two putative binding pockets for SA. A simulation in water of the complex between SA and the protein allowed us to determine that only one pocket—a surface cavity around Asn35—would efficiently bind SA in the presence of solvent. In silico mutagenesis and simulations of the ligand/protein complexes pointed to the importance of Asn35 and Arg81 in the binding of SA to GAPA1. The importance of this is further supported through experimental biochemical assays. Indeed, mutating GAPA1 Asn35 into Gly or Arg81 into Leu strongly diminished the ability of the enzyme to bind SA. The very same cavity is responsible for the NADP+ binding to GAPA1. More precisely, modelling suggests that SA binds to the very site where the pyrimidine group of the cofactor fits. NADH inhibited in a dose-response manner the binding of SA to GAPA1, validating our data.

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“…En la prueba de evaluación agronómica (PEA) las localidades y subregiones el clima y suelo fueron heterogéneas; no obstante, fueron más notorias las diferencias de clima en la altitud, temperatura y precipitación, factores que tienen una interacción compleja e inciden en el crecimiento y desarrollo y en los procesos de absorción, transporte de agua y nutrientes en la avena (Canales et al, 2019;Jinqiu et al, 2021;Rispail et al, 2018). De hecho, la altitud fue menor en la SRC (2066 msnm) y mayor en la SRN (3018 msnm), la temperatura fue mayor en la SRC con 17,2 o C y menor en la SRN con 13,7 o C y similar situación para la precipitación en SRC con 458 mm mayor a la ocurrida en la SRN con 342 mm.…”

Section: Discussionunclassified

“…La plasticidad fenotípica está reportada en varias especies [trigo (Triticum vulgare), sorgo (Sorghum bicolor L.), cebada (Hordeum vulgare L.) y arroz (Oryza sativa L.)] (Martínez-Sánchez, 2018), incluida la avena (Avena sativa L.) (Akçura & Çeri, 2011;Canales et al, 2019;Rispail et al, 2018;Sadras et al, 2017;Sánchez-Martín et al, 2015;2017). Los genotipos AV25-T y AV25-S mostraron mayor RMS y mejor calidad bromatológica del ensilaje (proteína, FDN y NTD).…”

Section: Discussionunclassified

Rendimiento e inclusión de ensilaje de avena forrajera (Avena sativa L.) AV25 en dietas para ganado lechero (Bos primigenius Taurus)

et al. 2021

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Introducción. Utilizar ensilaje de avena forrajera (Avena sativa L.) en la ganadería de leche en épocas deficitarias de alimento, permite mitigar pérdidas de biomasa por sequía en el trópico alto colombiano. Objetivos. 1) Determinar el rendimiento en materia seca (RMS) de cinco genotipos de avena forrajera y la estabilidad fenotípica y la calidad bromatológica de su ensilaje, y 2) evaluar la inclusión de ensilaje del genotipo AV25-T en dietas para ganado lechero. Materiales y métodos. Se realizaron en el trópico alto colombiano dos experimentos: 1) prueba de evaluación agronómica (octubre de 2016 a febrero de 2017) en un diseño de bloques completos al azar con cinco genotipos, ocho localidades y cuatro repeticiones; se determinó el rendimiento en materia seca (RMS) y en ensilaje los contenidos de proteína, fibra en detergente neutro (FDN) y nutrientes totales digestibles (NTD) y 2) estudio de respuesta animal (enero 2018) con la inclusión del genotipo AV25-T en dietas, en un diseño Crossover simple con dos factores: animal (5) y dietas (3); las dietas fueron: D1: solo pradera; D2: 35 % de ensilaje AV25-T + pastoreo y D3: 65 % de ensilaje AV25-T + pastoreo. Se determinó en la leche la producción, la proteína, la grasa y los sólidos totales, y en el animal el cambio de peso. Resultados. AV25-T presentó adaptación con el mayor RMS. La inclusión de ensilaje de AV25-T en la dieta animal, mostró un cambio significativo en el peso del animal (0,1 y 0,25 kg con D2 y D3), el rendimiento de leche (27,2 - 29,7 L/vaca/día con D3 y D2) y el contenido de grasa (3,2 - 3,9 % con D3 y D2), sin detrimento en proteína y sólidos totales. Conclusión. El uso del ensilaje de avena forrajera AV25-T permitió en vacas lecheras Holstein incrementos en el peso animal, el rendimiento de leche y el contenido grasa sin afectar la proteína y los sólidos totales.

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Salicylic acid regulates polyamine biosynthesis during drought responses in oat

Cited by 32 publications

References 20 publications

Deciphering Root Architectural Traits Involved to Cope With Water Deficit in Oat

Deciphering Root Architectural Traits Involved to Cope With Water Deficit in Oat

Deciphering the Binding of Salicylic Acid to Arabidopsis thaliana Chloroplastic GAPDH-A1

Rendimiento e inclusión de ensilaje de avena forrajera (Avena sativa L.) AV25 en dietas para ganado lechero (Bos primigenius Taurus)

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