Fatty acid profile of the sea snail Gibbula umbilicalis as a biomarker for coastal metal pollution

Silva, Carla O.; Simões, Tiago; Novais, Sara C.; Pimparel, Inês Carvalho; Granada, Luana; Soares, Amadeu M.V.M.; Barata, Carlos; Lemos, Marco F.L.

doi:10.1016/j.scitotenv.2017.02.015

Cited by 62 publications

(28 citation statements)

References 60 publications

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“…This increase maybe an adaptive response of treated organisms to face the detrimental effects. This fact is in accordance with a study of Silva et al [19], where levels of ARA in G. umbilicalis were observed to increase after metal exposure. Additionally, DGLA (20:3 n6) and EDA (20:2 n6), although clustered in a different group, presented an increase, particularly at 0.47% (C5).…”

Section: Discussionsupporting

confidence: 93%

“…The methodologies for FA preparation and analysis were performed according to Silva et al [19], with minor modifications. Briefly, for the initial saponification step, 150 µL of 2M KOH (diluted in 67% ethanol; v/v) was added to 150µl of homogenate.…”

Section: Fatty Acid Profilementioning

confidence: 99%

“…Fatty acid methyl ester mixes (PUFA No1 from Marine source and PUFA No 3 from Menhaden oil) were used as external standards (Supelco, Bellefonte, Pa., U.S.A.). Operating conditions were as described by Silva et al [19]. Theoretical correction factor (FCT) for FID detectors was applied in FA quantification, according to Guo [31].…”

Section: Fatty Acid Profilementioning

confidence: 99%

“…Fatty acid profile (FAP) has also been used as a biochemical response to pollutant exposure [18,19,20]. Fatty acids (FAs) are amongst the main constituents of the cell membrane and are involved in a wide range of biological pathways, from the production and permeability of cell membrane to lipids main components, while also being signalling mediators and used as fuel in all metabolic systems [21].…”

Section: Introductionmentioning

confidence: 99%

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<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva¹,

Simões²,

Félix³

et al. 2020

Preprint

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The red seaweed Asparagopsis armata exhibits a strong invasive behaviour and is included in the list of the “Worst invasive alien species threatening biodiversity in Europe”. This seaweed has been shown to produce a large diversity of halogenated compounds with effective biological effects, deeply affecting rockpool species. Therefore, the present study aimed to investigate the biochemical responses to sublethal concentrations of Asparagopsis armata exudate on two coastal organisms, the marine snail Gibbula umbilicalis and the rockpool shrimp Palaemon elegans. Antioxidant defences superoxide dismutase (SOD) and glutathione-S-transferase (GST), oxidative damage endpoints lipid peroxidation (LPO) and DNA damage, the neuronal parameter acetylcholinesterase (AChE), as well as the fatty acid profile were evaluated. Results revealed different metabolic responses between species, indicating that A. armata exudate affected the organisms through different pathways. Despite previous studies indicating that the exudate effected G. umbilicalis’ survival and behaviour, this does not seem to result from oxidative stress or addressed neurotoxicity. On the other hand, for P. elegans, an inhibition of AChE and the decrease of antioxidant capacity concomitant with the increase of LPO, suggests neurotoxicity and oxidative stress as mechanisms of exudate toxicity for this species. For fatty acids, there were different profile changes between species, also more pronounced for P. elegans with a general increase in PUFA with exudate exposure, which commonly means a defence mechanism protecting from membrane disruption. Nonetheless, the omega-3 PUFAs ARA and DPA were increased in both invertebrates, indicating a common mechanism regulation of inflammation and immunity responses to this stress. This work provides further insight on the mechanisms of invertebrate response and tolerance to an expanding coastal environmental stress as is the marine invader A. armata.

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Section: Discussionsupporting

confidence: 93%

Section: Fatty Acid Profilementioning

confidence: 99%

Section: Fatty Acid Profilementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva¹,

Simões²,

Félix³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fatty acid profile (FAP) has also been used as a biochemical response to pollutant exposure [18][19][20][21]. Fatty acids (FAs) are amongst the main constituents of the cell membrane and are involved in a wide range of biological pathways, from the production and permeability of cell membrane to lipids main components, while also being signaling mediators and used as fuel in all metabolic systems, having an important role on neural levels of biochemical and physiological response and in the processes of detoxification and inflammation [18].…”

Section: Introductionmentioning

confidence: 99%

Asparagopsis armata Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva

Simões

Félix

et al. 2021

Biology

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The seaweed Asparagopsis armata exhibits a strong invasive behavior, producing halogenated compounds with effective biological effects. This study addresses the biochemical responses to sublethal concentrations of A. armata exudate on the marine snail Gibbula umbilicalis whole body and the shrimp Palaemon elegans eyes and hepatopancreas. Antioxidant defenses superoxide dismutase (SOD) and glutathione-S-transferase (GST), oxidative damage endpoints lipid peroxidation (LPO) and DNA damage, the neuronal parameter acetylcholinesterase (AChE), and the fatty acid profile were evaluated. Results revealed different metabolic responses in both species. Despite previous studies indicating that the exudate affected G. umbilicalis’ survival and behavior, this does not seem to result from oxidative stress or neurotoxicity. For P. elegans, the inhibition of AChE and the decrease of antioxidant capacity is concomitant with the increase of LPO, suggesting neurotoxicity and oxidative stress as contributor mechanisms of toxicity for this species. Fatty acid profile changes were more pronounced for P. elegans with a general increase in polyunsaturated fatty acids (PUFAs) with the exudate exposure, which commonly means a defense mechanism protecting from membrane disruption. Nonetheless, the omega-3 PUFAs arachidonic acid (ARA) and docosapentaenoic acid (DPA) increased in both invertebrates, indicating a common regulation mechanism of inflammation and immunity responses.

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Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.

Doose

Fadhlaoui

Morin

et al. 2021

Enviro Toxic and Chemistry

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Aquatic ecotoxicological risks associated with tetravalent metallic elements such as thorium (Th) are still poorly understood. Periphytic biofilm represents an important food source in aquatic environments; thus, such risks could severely affect nutrient and energy cycling in these ecosystems. The present study investigated the potential for Th to change the fatty acid composition of biofilm communities. Bioaccumulation of Th and fatty acids were measured after 4 wk to 2 exposure conditions: a control (C0) and Th exposure (C10). Some major fatty acids such as C16:1n-7 and docosahexaenoic acid C22:6n-3 differed significantly between control and C10 conditions. To determine if Th can be trophically transferred and to investigate the impacts of nutritional quality changes on primary consumers, common pond snails (Lymnaea sp.) were fed for 4 wk with control and Th-exposed biofilm. Thorium appeared to be trophically transferable to the grazers, although we cannot exclude that part of the Th accumulated by the snails may have been taken from the water through release from the biofilms. The composition of major fatty acids observed in the grazers was also significantly affected, notably by a decrease of total polyunsaturated fatty acids. These results indicate that very low Th concentrations can decrease the nutritional quality of organisms at the base of the food chain.

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Fatty acid profile of the sea snail Gibbula umbilicalis as a biomarker for coastal metal pollution

Cited by 62 publications

References 60 publications

<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Asparagopsis armata Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.

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