1988
DOI: 10.1099/00207713-38-4-358
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Fatty Acids, Antibiotic Resistance, and Deoxyribonucleic Acid Homology Groups of Bradyrhizobium japonicum

Abstract: The fatty acid compositions and multiple antibiotic resistance patterns of 32 strains of Bradyrhizobium japonicum correlated with two major deoxyribonucleic acid homology groups. In group I, the fatty acid composition was 1.3% 16:l cis9 acid, 3.6% 16:lC acid, 8.8% 16:O acid, 1.2% 19:0 cyclopropane acid, and 81.2% 18:l acid. Group I1 contained 0.5% 16:lC acid, 11.1% 16:O acid, 0.8% 17:O cyclopropane acid, 24.7% 19:O cyclopropane acid, and 62.3% 18:l acid. Group I strains were susceptible to rifampin (500 pglml)… Show more

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Cited by 929 publications
(631 citation statements)
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“…Fatty acid methyl esters were obtained from wet biomass (ca. 40 mg) by saponification, methylation, and extraction (15). The fatty acid methyl ester mixtures were separated by using a model 5898A microbial identification system apparatus (Microbial ID, Newark, Del.).…”
Section: Taccgcggc[t/g]gctg3') 536f (5'cagc[c/a]gccgcggtaat[t/a]cmentioning
confidence: 99%
“…Fatty acid methyl esters were obtained from wet biomass (ca. 40 mg) by saponification, methylation, and extraction (15). The fatty acid methyl ester mixtures were separated by using a model 5898A microbial identification system apparatus (Microbial ID, Newark, Del.).…”
Section: Taccgcggc[t/g]gctg3') 536f (5'cagc[c/a]gccgcggtaat[t/a]cmentioning
confidence: 99%
“…Forty milligrams (wet weight) of cells was scraped from Petri dishes and fatty acid methyl esters were extracted using the method of Miller (1982) and Kuykendall et al (1988). Analyses were performed by the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ).…”
mentioning
confidence: 99%
“…Polar lipids were extracted and examined on 10Â10 cm TLC Silica gel 60 F 254 plates (Merck) (Minnikin et al, 1984;Ruan, 2006). Total cellular fatty acid methyl esters were prepared and determined by using GC/ MS (model GC-2010; Shi-madzu) (Kuykendall et al, 1988). Isoprenoid quinones were purified (Xu et al, 2007) by TLC and analysed by HPLC (Komagata & Suzuki, 1987).…”
mentioning
confidence: 99%