Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal

Pavićević, Ivan D.; Jovanović, Vesna; Takić, Marija; Penezić, Ana; Aćimović, Jelena M.; Mandić, Ljuba M.

doi:10.1016/j.cbi.2014.10.008

Cited by 30 publications

(25 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several reports have presented the synthesis of inorganic nanoparticles with the presence of thiols, such as glutathione 31 - 33 . Many free thiols exist in albumin due to the presence of cysteine-34 free thiol groups 34 . Thus, albumin has been explored as a template to synthesize fluorescent metal clusters and inorganic nanocrystals 35 - 38 .…”

Section: Albumin As a Template For Nanoparticle Preparation And Its Bmentioning

confidence: 99%

Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery

2017

View full text Add to dashboard Cite

Biosafety is the primary concern in clinical translation of nanomedicine. As an intrinsic ingredient of human blood without immunogenicity and encouraged by its successful clinical application in Abraxane, albumin has been regarded as a promising material to produce nanoparticles for bioimaging and drug delivery. The strategies for synthesizing albumin-based nanoparticles could be generally categorized into five classes: template, nanocarrier, scaffold, stabilizer and albumin-polymer conjugate. This review introduces approaches utilizing albumin in the preparation of nanoparticles and thereby provides scientists with knowledge of goal-driven design on albumin-based nanomedicine.

show abstract

Section: Albumin As a Template For Nanoparticle Preparation And Its Bmentioning

confidence: 99%

Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery

2017

View full text Add to dashboard Cite

show abstract

“…Due to its high abundance in serum, its role as endogenous antioxidant compound (Rondeau et al, 2010) and long half-life (19 days) (Pavicevic et al, 2014), human serum albumin (HSA) is highly exposed to oxidative stress (Chesne, Rondeau, Armenta, & Bourdon, 2006) and vulnerable to glycation process under hyperglycemic conditions (Chayaratanasin et al, 2015) which may be used as a marker of glycation reaction (Pavicevic et al, 2014). It is interesting to cite that glycation of HSA-induced depletion in lysine, arginine, and Cysteine residues in diabetes (Arasteh, Farahi, Habibi-Rezaei, & Moosavi-Movahedi, 2014).…”

Section: Glycation Reaction Induces Modifications Of Protein Second-mentioning

confidence: 99%

Glucose‐mediated protein glycation: Contribution of methanolic extract of Ceratonia siliqua L. in protection and in vitro potential inhibition of acetylcholinesterase

et al. 2019

View full text Add to dashboard Cite

Chronic hyperglycemia presents the major etiology of diabetes mellitus and related complications mainly Alzheimer's disease, via the protein glycation and toxic products generated. In the current study, we investigated the eventual protective effect of the methanolic extract of Ceratonia siliqua L. (CsME) against glucose‐mediated glycation in serum bovine albumin. The multi‐stage glycation markers, namely fructosamines and advanced glycation end products (AGEs) levels were monitored along with measurement of thiol groups; moreover, the in vitro acetylcholinesterase (AChE) inhibition potential was carried out. HPLC was also assessed. Rutin was the main phenolic compound found in CsME. CsME showed a good capacity to inhibit AGEs, fructosamines and protected thiol groups against glycation. CsME exhibited a great AChE inhibition activity. In the present study, CsME prevented glucose‐induced protein glycation, it also exhibited a good inhibition of AChE, suggesting its DM complications such as memory troubles related to AD. Practical applications Neurodegenerative disorders ranging from memory troubles to Alzheimer's disease present the most diabetes mellitus complications and mainly attributed to protein glycation process. Currently, there is a strong trend to search for efficient natural sources of glycation and acetylcholinesterase inhibitors to replace the synthetic ones, whose secondary effects were shown. The present article tries to justify scientifically the wide use of Ceratonia siliqua L. in Moroccan folk medicine, demonstrating that the methanolic extract of leaves from this species presents a promising source of new natural compounds inhibiting acetylcholinesterase and acting in vitro against glycation generated compounds. Furthermore, for the first time, Rutin was the main phenolic compound found in this extract, these encouraging results should be coupled with further studies to integrate it in pharmaceutical formulations. As such, this paper should be of interest to a broad readership, including those interested in Biochemistry, Phytochemistry, pharmacology, and neurosciences.

show abstract

“…Fatty acid binding modulates the reactivity of HSA via the –SH group at the Cys34, but, interestingly, FA binding is not at this Cys34 thiol group . This configuration permits synergistic changes in scavenger capacity and antioxidant properties when fatty acids are bound to HSA.…”

Section: Albumin Binding: More Than Just a Carrier?mentioning

confidence: 99%

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

et al. 2016

View full text Add to dashboard Cite

Albumin is a major plasma protein that has become ubiquitous in regenerative medicine research. As such, many studies have examined its structure and advantageous properties. However, a systematic and comprehensive understanding of albumin's role, capabilities and therapeutic potential still eludes the field. In the present work, we review how albumin is applied in tissue engineering, including cell culture and storage, in vitro fertilization and transplantation. Furthermore, we discuss how albumin's physiological role extends beyond a carrier for metal ions, fatty acids, pharmacons and growth factors. Albumin acts as a bacteriostatic coating that simultaneously promotes attachment and proliferation of eukaryotic cells. These properties with the combination of free radical scavenging, neutrophil activation and as a buffer molecule already make the albumin protein beneficial in healing processes supporting functional tissue remodeling. Nevertheless, recent data revealed that albumin can be synthesized by osteoblasts and its local concentration is raised after bone trauma. Interestingly, by increasing the local albumin concentration in vivo, faster bone healing is achieved, possibly because albumin recruits endogenous stem cells and promotes the growth of new bone. These data also suggest an active role of albumin, even though a specific receptor has not yet been identified. Together, this discussion sheds light on why the extravascular use of the albumin molecule is in the scope of scientific investigations and why it should be considered as a local therapeutic agent in regenerative medicine. © 2016 BioFactors, 43(3):315-330, 2017.

show abstract

Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal

Cited by 30 publications

References 52 publications

Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery

Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery

Glucose‐mediated protein glycation: Contribution of methanolic extract of Ceratonia siliqua L. in protection and in vitro potential inhibition of acetylcholinesterase

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

Contact Info

Product

Resources

About