2015
DOI: 10.1128/jvi.00115-15
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Faustovirus, an Asfarvirus-Related New Lineage of Giant Viruses Infecting Amoebae

Abstract: Giant viruses are protist-associated viruses belonging to the proposed order Megavirales; almost all have been isolated from Acanthamoeba spp. Their isolation in humans suggests that they are part of the human virome. Using a high-throughput strategy to isolate new giant viruses from their original protozoan hosts, we obtained eight isolates of a new giant viral lineage from Vermamoeba vermiformis, the most common free-living protist found in human environments. This new lineage was proposed to be the faustovi… Show more

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Cited by 176 publications
(255 citation statements)
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“…The capsid protein of faustovirus E12 was extracted and analyzed by SDS/PAGE, followed by Western blotting according to the protocol described by Azza et al (5). Identification of the peptide sequence was performed by matrix-assisted laser desorption ionization time-of-flight MS and nano-LC-MS/MS as described by Reteno et al (2).…”
Section: Methodsmentioning
confidence: 99%
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“…The capsid protein of faustovirus E12 was extracted and analyzed by SDS/PAGE, followed by Western blotting according to the protocol described by Azza et al (5). Identification of the peptide sequence was performed by matrix-assisted laser desorption ionization time-of-flight MS and nano-LC-MS/MS as described by Reteno et al (2).…”
Section: Methodsmentioning
confidence: 99%
“…Primers for PCR amplification and sequencing (Table S1) were designed using PrimerQuest in the area containing the capsid sequences predicted by the preliminary bioinformatic study (2).…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations