Favorable effects of the weak ethylene receptor mutation Sletr1-2 on postharvest fruit quality changes in tomatoes

Abstract

Tomatoes with a prolonged fruit shelf life and improved postharvest quality would be an attractive commodity for both breeders and consumers. A weak allele of the tomato ethylene receptor mutant Sletr1-2 elicits an extended fruit shelf life without prominent undesirable pleiotropic effects. In this study, we elucidate the influences of the Sletr1-2 mutation on alterations of the postharvest fruit quality of Sletr1-2 F1 hybrid lines from four different pure-line cultivar parents. The changes in the compositions of metabolic compounds, including sugars, organic acids and amino acids, over 30 days of postharvest storage have been evaluated. The Sletr1-2 mutation significantly affected the postharvest fruit quality parameters of the Sletr1-2 F1 hybrid lines in a manner that depended on the pure-line cultivar parental backgrounds. The influence of the Sletr1-2 mutation was detected only in the reductions and/or increases of individual amino acids and increases in the levels of organic acids, i.e., malate and citrate. In contrast, the sugar level was not changed. Moreover, the Sletr1-2 mutation significantly reduced the rate of water loss during postharvest storage. These results indicate that the Sletr1-2 mutation has favorable effects on the postharvest changes of Sletr1-2 F1 hybrid tomatoes that improve both the shelf life and the taste quality.

Evidence of the functional role of the ethylene receptor genes SlETR4 and SlETR5 in ethylene signal transduction in tomato

“…For fresh market purposes, extending fruit shelf life by 2 days is only beneficial for nearby markets. Prolonged fruit shelf life is important for long-distance transportation to markets, fruit storage, and handling (Mubarok et al 2015;Mubarok et al 2016). In contrast with the Sletr4-1 mutant line, Sletr5-1 exhibited an accelerated fruit ripening process.…”

An overview of ethylene insensitive tomato mutants: Advantages and disadvantages for postharvest fruit shelf-life and future perspective

“…There is a change in the total sugar content during fruit maturation that can be used to determine the sweetness of tomato fruits . The entire sugar content of the mutant Sletr1-1 and Sletr1-2, both in homozygous and heterozygous form, is lower than WT-MT, except for the F1 Sletr1-2 heterozygous (Mubarok et al, 2016). The fruit of nor mutant has the lowest total sugar content, followed by the fruit of Nr, rin, Sletr1-1, and Sletr1-2 (Osorio et al, 2011;Mubarok et al, 2016;Osorio et al, 2020).…”

“…The entire sugar content of the mutant Sletr1-1 and Sletr1-2, both in homozygous and heterozygous form, is lower than WT-MT, except for the F1 Sletr1-2 heterozygous (Mubarok et al, 2016). The fruit of nor mutant has the lowest total sugar content, followed by the fruit of Nr, rin, Sletr1-1, and Sletr1-2 (Osorio et al, 2011;Mubarok et al, 2016;Osorio et al, 2020). The difference in the sensitivity to ethylene can cause the variation in sugar content in these mutants.…”

“…Oms-Oliu et al (2011) stated that the metabolisms of citrate and malate are subjected to ethylene regulation. Several factors affect the levels of organic acids in tomato fruit, and ethylene is one of the influencing factors (Mubarok et al, 2016). The change of organic acid content in fruit is directly affected by the function of ethylene response.…”

Identifikasi Dan Karakterisasi 11 Kultivar Tanaman Tomat Sebagai Sumber Genetik Untuk Persilangan

“…dalam Marliah, dkk. (2012) dalam setiap 100 g buah tomat mengandung 4,2 g karbohidrat, 0,3 g lemak, 1 g protein, 5 mg kalsium, 27 mg fosfor, 0,5 mg zat besi, 1500 Si vitamin A (karoten), 60 mg vitamin B (tiamin), dan 40 mg vitamin C. Selain gizi yang terkandung dalam buah, tomat pun mempunyai rasa enak yang dipengaruhi oleh kandungan gula, asam organik dan asam amino (Mubarok et al, 2016).…”