Fc fragment from human IgG induces PGE2 secretion

Carosella, Edgardo D.; Pascal, N.; Armand, J

doi:10.1016/0008-8749(89)90024-5

Cited by 5 publications

(1 citation statement)

References 26 publications

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“…We removed the cells that did not adhere, while the adherent cells were carefully washed at least twice with warm PBS (Biochrom AG) before being harvested. The purity of the resulting cell suspension was then randomly tested using fluorescence-activated cell sorter (FACS) analysis; the sample was deemed pure if it yielded at least 95% monocytes as previous described [ 30 ]. Afterward, various concentrations (5, 25, 250, and 2500 mg/dL) of IVIG (7S-IVIG; Gamimmune-N, Bayer, USA) or recombinant PGE 2 (0, 10 −6 , 10 −5 , and 10 -4 M, R&D) were incorporated into the PBMC (with monocyte isolation) and PBMC (without monocyte isoloation) for assay of PGE2 and CD40L expression on CD4+ T cells, in a time series of 0.5, 1, 6, 24, and 48 hours, respectively.…”

Section: Methodsmentioning

confidence: 99%

Plasma Prostaglandin E2 Levels Correlated with the Prevention of Intravenous Immunoglobulin Resistance and Coronary Artery Lesions Formation via CD40L in Kawasaki Disease

et al. 2016

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BackgroundA form of systemic vasculitis, Kawasaki disease (KD) occurs most frequently in children under the age of five years old. Previous studies have found that Prostaglandin E2 (PGE2) correlates with KD, although the related mechanisms are still unknown. CD40L may also be a marker of vasculitis in KD, so this study focuses on PGE2 and CD40L expression in KD.Materials and MethodsThis study consisted of a total of 144 KD patients, whose intravenous immunoglobulin (IVIG)/coronary arterial lesion (CAL) formation resistance was evaluated. PGE2 levels were evaluated in vitro to study the effect of CD40L on CD4+ T lymphocytes.ResultsPGE2 levels significantly increased after IVIG treatment (p<0.05), especially in patients who responded to initial IVIG treatment (p = 0.004) and for patients without CAL formation (p = 0.016). Furthermore, an in vitro study revealed that IVIG acted as a trigger for PGE2 expression in the acute-stage mononuclear cells of KD patients. According to our findings, both IVIG and PGE2 can impede surface CD40L expressions on CD4+ T lymphocytes (p<0.05).ConclusionsThe results of this study are among the first to find that plasma PGE2 is correlated with the prevention of IVIG resistance and CAL formation through CD40L in KD.

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Section: Methodsmentioning

confidence: 99%