Fcγ Receptor Regulation of Citrobacter rodentium Infection

Gamma Interferon Produced by Antigen-Specific CD4+T Cells Regulates the Mucosal Immune Responses toCitrobacter rodentiumInfection

“…To assess the host antigen-specific T-cell responses during C. rodentium infection, C. rodentium constitutively expressing OVA was generated as previously described (13,27). As shown in Fig.…”

“…The protective effects of IgG might contribute to the complementfixing activity of high-affinity binding to activated Fc␥Rs, such as Fc␥RI and Fc␥RII/III (13,18). Bacterium-specific IgG plays FIG.…”

“…C. rodentium strain DBS100 (catalog number 51459; ATCC) was kindly provided by Gad Frankel (Division of Cell and Molecular Biology, Imperial College London, United Kingdom). Constitutively OVA-expressing C. rodentium (OVA-C. rodentium) was created by electroporation of a plasmid vector encoding the chicken OVA construct (28) under the control of the two gal operon promoters and a kanamycin resistance gene into C. rodentium strain DBS100 (13). Green fluorescent protein (GFP)-expressing C. rodentium (GFP-C. rodentium) was kindly provided by C. Sasakawa (Tokyo University, Japan).…”

“…Macrophages were prepared as described previously (13). Briefly, C57BL/6 mice were injected with 1 ml of 3% thioglycolate into the peritoneal cavity.…”

The role of specific IgG and complement in combating aprimary mucosal infection of the gut epithelium

“…However, this question is critical not only for the study of attaching and effacing pathogens, but for the development of systemic/IgG-based vaccines that serve to protect the host prior to, or during early phases of infections involving mucosal contact. Studies by Masuda et al [18] have previously shown the importance of Fc-receptor-bearing cells in facilitating survival and clearance of C. rodentium, suggesting that opsonization and uptake of the pathogen, or pathogen antigens by macrophages and DCs, contribute to host defense.…”