Fe3O4/Au/Fe3O4nanoflowers exhibiting tunable saturation magnetization and enhanced bioconjugation

Wang, Hui; Shi, Feng; Yan, Kunping; Peng, Mingli; Cheng, Xiao; Luo, Yan‐Ling; Chen, Xuemei; Roy, V. A. L.; Cui, Yali; Wang, Zuankai

doi:10.1039/c2nr11489e

Cited by 35 publications

(34 citation statements)

References 41 publications

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“…The different structures with core/shell, dumbbell-like and flower-shape of these nanoparticles have been reported. [27][28][29] Herein, the Fe 3 O 4 /Au/Fe 3 O 4 configuration (nanoflowers) with 38 nm diameter was synthesized 30 and used as a carrier to set up a device for SNP detection. Compared to other matrix nanoparticles, our patented PAA ( poly-acrylic acid) modification of GoldMag nanoparticles ensures the significantly elevated stableness and compatibility of the particles for biological interactions.…”

Section: Introductionmentioning

confidence: 99%

A novel lateral flow assay based on GoldMag nanoparticles and its clinical applications for genotyping of MTHFR C677T polymorphisms

Wang

Zhang

et al. 2016

Nanoscale

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Current techniques for single nucleotide polymorphism (SNP) detection require tedious experimental procedures and expensive and sophisticated instruments. In this study, a visual genotyping method has been successfully established via combining ARMS-PCR with gold magnetic nanoparticle (GoldMag)-based lateral flow assay (LFA) and applied to the genotyping of methylenetetrahydrofolate reductase (MTHFR) C677T. C677T substitution of the gene MTHFR leads to an increased risk of diseases. The genotyping result is easily achievable by visual observation within 5 minutes after loading of the PCR products onto the LFA device. The system is able to accurately assess a broad detection range of initial starting genomic DNA amounts from 5 ng to 1200 ng per test sample. The limit of detection reaches 5 ng. Furthermore, our PCR-LFA system was applied to clinical trials for screening 1721 individuals for the C677T genotypes. The concordance rate of the genotyping results detected by PCR-LFA was up to 99.6% when compared with the sequencing results. Collectively, our PCR-LFA has been proven to be rapid, accurate, sensitive, and inexpensive. This new method is highly applicable for C677T SNP screening in laboratories and clinical practices. More promisingly, it could also be extended to the detection of SNPs of other genes.

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Section: Introductionmentioning

confidence: 99%

A novel lateral flow assay based on GoldMag nanoparticles and its clinical applications for genotyping of MTHFR C677T polymorphisms

Wang

Zhang

et al. 2016

Nanoscale

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“…GoldMag nanoparticles were synthesized and characterized as described previously (21). The general scheme to functionalize nanoflower GMNs with CTAB and PSS coating, is represented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…GoldMag nanoparticles (5 mg/ml) with a nanoflower structure (21) and lateral flow strips were provided by Xi'an GoldMag Nanobiotech Co., Ltd. (Xi'an, China). Buffers were prepared according to standard laboratory procedures.…”

Section: Methodsmentioning

confidence: 99%

Apolipoprotein E genotyping using PCR-GoldMag lateral flow assay and its clinical applications

Lian

Wang

et al. 2016

Molecular Medicine Reports

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A polymerase chain reaction-gold magnetic nanoparticles lateral flow assay (PCR-GoldMag LFA) has been developed via integrating multiplex amplification refractory mutation system PCR (multi-ARMS-PCR) with GoldMag-based LFA for the visual detection of single-nucleotide polymorphisms (SNPs). This assay was applied to genotype Apolipoprotein E (ApoE). ApoE genotyping is important due to the predictive value for the development of coronary artery disease and Alzheimer's disease. The method requires two steps: i) Simultaneous amplifications of the two polymorphic codons (ApoE 158 and 112), performed in separated reactions using multi-ARMS-PCR; and ii) detection of the wild-type and mutant PCR products via dual immunoreactions, which can be performed in ~5 min. Within two LFAs, anti-digoxin antibody-conjugated GoldMag probes bind digoxin-labeled wild-type PCR products, and anti-fluorescein isothiocyanate (FITC) antibody-conjugated GoldMag probes bind FITC-labeled mutant PCR products. All PCR products are biotin labeled and are detected by streptavidin-coated regions on the LFA strip, resulting in a red color. The current approach is capable of detecting the SNPs of ApoE in ~1.5 h, with a broad detection range from 10–1,000 ng of genomic DNA. Thus, the present protocol may facilitate simple, fast and cost-effective screening for important SNPs, as demonstrated by the evaluation of the prevalence of ApoE variants in a Han Chinese cohort.

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“…97 The dendritic Fe 3 O 4 @Au NPs show a mean particle size of 35 nm, intense near-infrared (NIR) absorbance at 754 nm, and strong superparamagnetism, implying the potential applications in the fields of the optical/magnetic-resonance bimodal imaging, photothermal-magnetothermal combined therapy, low temperature catalysis, and magnetic separation. Hui et al 98 reported a convenient method for synthesis of novel Fe 3 O 4 @Au@Fe 3 O 4 nanoflowers that integrate hybrid components and surface structures. Relative to conventional NPs with the core-shell configuration, such nanoflowers not only retain their surface plasmon properties but also allow for a 170% increase in the saturation magnetization and a 23% increase in the conjugation efficiency because of the synergistic cooperation among the hierarchical structures.…”

Section: Structure and Synthesis Of Magnetoplasmonic Nanoparticlesmentioning

confidence: 99%

Multifunctional Magnetoplasmonic Nanomaterials and Their Biomedical Applications

Zhou¹,

Zou²,

Koh³

et al. 2014

j biomed nanotechnol

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Multifunctional magnetoplasmonic (Au-Fe x O y ) nanomaterials are characterized by some features of iron oxide and gold, such as surface chemistry, special optical properties, and superparamagnetic properties, which have helped to draw much attention to their biomedical applications. In this review, the state of the art of this rapidly developing field is described. We review the developments in different approaches to integrating the magnetic and plasmonic properties in a single nanoparticle with different morphological traits. Specific plasmonic and magneto-optical properties of magnetoplasmonic nanoparticles are explained; this information should shed light on the future development of multifunctional magnetoplasmonic nanomaterials. We also review cytotoxicity of magnetoplasmonic nanoparticles by in vitro and in vivo studies. With the multifunctional properties of magnetoplasmonic nanomaterials, a variety of applications such as biosensor, bioseparation, multimodal imaging, and therapeutics is possible and is highlighted here, in addition to outlining the future trends and perspectives of these sophisticated nanocomposites.

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Fe₃O₄/Au/Fe₃O₄nanoflowers exhibiting tunable saturation magnetization and enhanced bioconjugation

Cited by 35 publications

References 41 publications

A novel lateral flow assay based on GoldMag nanoparticles and its clinical applications for genotyping of MTHFR C677T polymorphisms

A novel lateral flow assay based on GoldMag nanoparticles and its clinical applications for genotyping of MTHFR C677T polymorphisms

Apolipoprotein E genotyping using PCR-GoldMag lateral flow assay and its clinical applications

Multifunctional Magnetoplasmonic Nanomaterials and Their Biomedical Applications

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