Fe2+-induced lipid peroxidation kinetics in liposomes: The role of surface Fe2+ concentration in switching the reaction from acceleration to decay

Driomina, Elena S.; Sharov, Victor S.; Vladimirov, Yu. A.

doi:10.1016/0891-5849(93)90070-b

“…In our previous report it was found that the concentration of lipid material influences strongly the kinetics of Fe 2 + -induced LPO and associated CL in a liposome suspension (6). Similar effects are reproduced in Fig.…”

Section: The Role Of Lipid Materialssupporting

confidence: 81%

“…The concentration of oxygen is crucial for the lipid peroxidation rate (7). The nature of reactions occurring at these CL stages (fast flash, latent period, and slow flash) has been investigated and reported in previous publications (3,6,7). The rate of the stirrer rotation was chosen so that further increase in the rotation rate did not influence the CL kinetics.…”

Section: Methodsmentioning

confidence: 99%

“…The kinetics of LPO and CL in such a system is comparatively complex and includes several stages: (1) fast flash of CL produced by decomposition of lipid peroxides pre-existing in the suspension; (2) latent period whose duration depends on Fe 2 + and other antioxidant concentrations; (3) slow flash of CL; and (4) SL. The first three stages of the process were a matter of investigation in our previous reports where reaction kinetics and the mechanism of Fe 2 +-catalysed LPO reactions have been thoroughly considered (3,6,7).…”

Section: Introductionmentioning

confidence: 99%

“…According to the reaction scheme, CL intensity I = <pk 6 [LOO . According to the reaction scheme, CL intensity I = <pk 6 [LOO .…”

Section: Introductionmentioning

confidence: 99%

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Two Processes Responsible for Chemiluminescence Development in the Course of Iron‐Mediated Lipid Peroxidation

Sharov¹,

Driomina²,

Vladimirov³

1996

J. Biolumin. Chemilumin.

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The kinetics of chemiluminescence (CL) accompanying Fe(2+)-induced lipid peroxidation (LPO) in liposome suspension has been investigated. A sequence of stages was observed, namely: (1) fast CL flash (FF); (2) latent period (LP); (3) slow CL flash (SF) and (4) stationary chemiluminescence (SL). The first three stages are known to reflect the Fe(2+)-mediated LPO process. In spite of the fact that at the stage of SL Fe2+ has completely oxidized and MDA has not accumulated, CL intensity was found to increase and after 0.5-1 h reached a value that was several times higher than SF amplitude. The maximal SL level was linearly dependent on the initial Fe2+ concentration and was not dependent on liposome concentration in the suspension. The nature of the processes responsible for CL emission at the stage of SL has been investigated using free radical reaction inhibitors and measurement of CL spectra. The SL spectrum was observed in the red region (lamda > 590 nm) in contrast to the SF CL spectrum (maximum at 540nm). SL amplitude was strongly inhibited by sodium azide (40%), superoxide dismutase (SOD) (30%), desferrioxamine and EDTA (30%), whereas mannitol, ethanol, alpha-tocopherol and butylated hydroxytoluene were ineffective. The data obtained indicate that CL at the stage of SL is not directly related to LPO process, i.e. lipid free radical recombination. The mechanism of stationary CL generation is discussed.

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“…In addition, in the presence of transition metals lipid hydroperoxides induce LPO branching [2,16]. In addition, in the presence of transition metals lipid hydroperoxides induce LPO branching [2,16].…”

mentioning

confidence: 99%

Chemiluminescence study of the contents of the products of Cu2+-induced lipid peroxidation in different fractions of human blood lipoproteins

Dremina

¹

,

Вахрушева

²

,

Sharov

³

et al. 1995

Bull Exp Biol Med

0

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It is demonstrated that the content of the primary products of lipid peroxidation reaches the maximum after about 1-h incubation with Cu 2 § and then declines. At a Cu 2 § concentration of about 10=15 ~tM, the content of lipid peroxidation products is maximal; it does not rise with a further increase in the Cu 2. concentration. Comparison of the kinetics of lipid peroxidation in different lipoprotein fractions shows that low density lipoproteins are much more strongly oxidized than high density lipoproteins. A strong positive correlation between the amplitude of the chemiluminescence burst and the diene conjugate content is established in 79 independent measurements.

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Two Processes Responsible for Chemiluminescence Development in the Course of Iron-Mediated Lipid Peroxidation

Sharov

¹

,

Driomina

²

,

Vladimirov

³

1996

J. Biolumin. Chemilumin.

Self Cite

View full text Add to dashboard Cite

The kinetics of chemiluminescence (CL) accompanying Fe(2+)-induced lipid peroxidation (LPO) in liposome suspension has been investigated. A sequence of stages was observed, namely: (1) fast CL flash (FF); (2) latent period (LP); (3) slow CL flash (SF) and (4) stationary chemiluminescence (SL). The first three stages are known to reflect the Fe(2+)-mediated LPO process. In spite of the fact that at the stage of SL Fe2+ has completely oxidized and MDA has not accumulated, CL intensity was found to increase and after 0.5-1 h reached a value that was several times higher than SF amplitude. The maximal SL level was linearly dependent on the initial Fe2+ concentration and was not dependent on liposome concentration in the suspension. The nature of the processes responsible for CL emission at the stage of SL has been investigated using free radical reaction inhibitors and measurement of CL spectra. The SL spectrum was observed in the red region (lamda > 590 nm) in contrast to the SF CL spectrum (maximum at 540nm). SL amplitude was strongly inhibited by sodium azide (40%), superoxide dismutase (SOD) (30%), desferrioxamine and EDTA (30%), whereas mannitol, ethanol, alpha-tocopherol and butylated hydroxytoluene were ineffective. The data obtained indicate that CL at the stage of SL is not directly related to LPO process, i.e. lipid free radical recombination. The mechanism of stationary CL generation is discussed.

show abstract

Fe2+-induced lipid peroxidation kinetics in liposomes: The role of surface Fe2+ concentration in switching the reaction from acceleration to decay

Cited by 26 publications

References 27 publications

Two Processes Responsible for Chemiluminescence Development in the Course of Iron‐Mediated Lipid Peroxidation

Two Processes Responsible for Chemiluminescence Development in the Course of Iron‐Mediated Lipid Peroxidation

Chemiluminescence study of the contents of the products of Cu2+-induced lipid peroxidation in different fractions of human blood lipoproteins

Two Processes Responsible for Chemiluminescence Development in the Course of Iron-Mediated Lipid Peroxidation

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