Fear conditioning-related changes in cerebellar Purkinje cell activities in goldfish

Yoshida, Masayuki; Kondo, Hiroki

doi:10.1186/1744-9081-8-52

Cited by 19 publications

(10 citation statements)

References 32 publications

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“…However, the suppression of simple spikes of Purkinje-cell activity during eyeblink conditioning in mammals is also reported 42 , 43 . Although fear conditioning in goldfish increases the simple spikes in some Purkinje cells, more of these cells show a reduction in simple spikes 44 , suggesting that in teleosts, Purkinje-cell activity is mostly suppressed during fear conditioning. The reduced Purkinje-cell activity probably upregulates the activity of the projection neurons, which may be responsible for expressing the conditioned fear responses.…”

Section: Discussionmentioning

confidence: 97%

Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum

Matsuda

Yoshida

Kikuchi

et al. 2017

Sci Rep

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Although previous studies show that the cerebellum is involved in classical fear conditioning, it is not clear which components in the cerebellum control it or how. We addressed this issue using a delayed fear-conditioning paradigm with late-stage zebrafish larvae, with the light extinguishment as the conditioned stimulus (CS) and an electric shock as the unconditioned stimulus (US). The US induced bradycardia in the restrained larvae. After paired-associate conditioning with the CS and US, a substantial population of the larvae displayed CS-evoked bradycardia responses. To investigate the roles of the zebrafish cerebellum in classical fear conditioning, we expressed botulinum toxin or the Ca2+ indicator GCaMP7a in cerebellar neurons. The botulinum-toxin-dependent inhibition of granule-cell transmissions in the corpus cerebelli (CCe, the medial lobe) did not suppress the CS-evoked bradycardia response, but rather prolonged the response. We identified cerebellar neurons with elevated CS-evoked activity after the conditioning. The CS-evoked activity of these neurons was progressively upregulated during the conditioning and was downregulated with repetition of the unpaired CS. Some of these neurons were activated immediately upon the CS presentation, whereas others were activated after a delay. Our findings indicate that granule cells control the recovery from conditioned fear responses in zebrafish.

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Section: Discussionmentioning

confidence: 97%

Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum

Matsuda

Yoshida

Kikuchi

et al. 2017

Sci Rep

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“…We found that the IO is not associated with nociceptive reflexes in the tail-flick test but is important in the tachycardiac response to the US. A previous study on the acquisition of fear-conditioned bradycardia in goldfish found that cerebellar Purkinje cells have a simple spike response to the CS and complex spike response to the US 21 . Thus, inputs from the IO to the cerebellar cortex and/or cerebellar nuclei are thought to be involved in tachycardiac responses to the US.…”

Section: Discussionmentioning

confidence: 97%

Effects of inferior olive lesion on fear-conditioned bradycardia

Kotajima

Sakai²,

Hashikawa³

et al. 2014

NeuroReport

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The inferior olive (IO) sends excitatory inputs to the cerebellar cortex and cerebellar nuclei through the climbing fibers. In eyeblink conditioning, a model of motor learning, the inactivation of or a lesion in the IO impairs the acquisition or expression of conditioned eyeblink responses. Additionally, climbing fibers originating from the IO are believed to transmit the unconditioned stimulus to the cerebellum in eyeblink conditioning. Studies using fear-conditioned bradycardia showed that the cerebellum is associated with adaptive control of heart rate. However, the role of inputs from the IO to the cerebellum in fear-conditioned bradycardia has not yet been investigated. To examine this possible role, we tested fear-conditioned bradycardia in mice by selective disruption of the IO using 3-acetylpyridine. In a rotarod test, mice with an IO lesion were unable to remain on the rod. The number of neurons of IO nuclei in these mice was decreased to ∼40% compared with control mice. Mice with an IO lesion did not show changes in the mean heart rate or in heart rate responses to a conditioned stimulus, or in their responses to a painful stimulus in a tail-flick test. However, they did show impairment of the acquisition/expression of conditioned bradycardia and attenuation of heart rate responses to a pain stimulus used as an unconditioned stimulus. These results indicate that the IO inputs to the cerebellum play a key role in the acquisition/expression of conditioned bradycardia.

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“…Adult teleost fish classically condition readily (Agranoff and Davis, 1968; Flood et al, 1976; Amiro and Bitterman, 1980; Mattioli et al, 1998; Eisenberg et al, 2003; Salas et al, 2006; Yoshida and Kondo, 2012), and there have been several published reports of classical conditioning in adult zebrafish (Braubach et al, 2009; Agetsuma et al, 2010; Karnik and Gerlai, 2012; Aoki et al, 2013). To date, there have been just two reports of successful classical conditioning in larval to juvenile zebrafish.…”

Section: Types Of Learning and Memory In Larval Zebrafishmentioning

confidence: 99%

Learning and memory in zebrafish larvae

Roberts¹,

Bill²,

Glanzman³

2013

Front. Neural Circuits

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Larval zebrafish possess several experimental advantages for investigating the molecular and neural bases of learning and memory. Despite this, neuroscientists have only recently begun to use these animals to study memory. However, in a relatively short period of time a number of forms of learning have been described in zebrafish larvae, and significant progress has been made toward their understanding. Here we provide a comprehensive review of this progress; we also describe several promising new experimental technologies currently being used in larval zebrafish that are likely to contribute major insights into the processes that underlie learning and memory.

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Fear conditioning-related changes in cerebellar Purkinje cell activities in goldfish

Cited by 19 publications

References 32 publications

Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum

Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum

Effects of inferior olive lesion on fear-conditioned bradycardia

Learning and memory in zebrafish larvae

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