Feasibility and efficacy of bone tissue engineering using human bone marrow stromal cells cultivated in serum-free conditions

Agata, Hideki; Watanabe, Nobuyuki; Ishii, Yumiko; Kubo, Noriyuki; Ohshima, Satoshi; Yamazaki, Masahito; Tojo, Arinobu; Kagami, Hideaki

doi:10.1016/j.bbrc.2009.03.023

Cited by 78 publications

(68 citation statements)

References 15 publications

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“…In the future it is possible that serum-free media 45 or media supplemented with human platelet lysate 46 will be used to culture the cells on the carrier. However, there are as yet no xenobiotic-free media approved for clinical use.…”

Section: Discussionmentioning

confidence: 99%

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Walker

Mistry²,

Smith³

et al. 2012

Tissue Engineering Part C: Methods

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Skin has a remarkable capacity for regeneration, but age-and diabetes-related vascular problems lead to chronic non-healing wounds for many thousands of U.K. patients. There is a need for new therapeutic approaches to treat these resistant wounds. Donor mesenchymal stem/stromal cells (MSCs) have been shown to assist cutaneous wound healing by accelerating re-epithelialization. The aim of this work was to devise a low risk and convenient delivery method for transferring these cells to wound beds. Plasma polymerization was used to functionalize the surface of medical-grade silicone with acrylic acid. Cells attached well to these carriers, and culture for up to 3 days on the carriers did not significantly affect their phenotype or ability to support vascular tubule formation. These carriers were then used to transfer MSCs onto human dermis. Cell transfer was confirmed using an MTT assay to assess viable cell numbers and enhanced green fluorescent protein-labeled MSCs to demonstrate that the cells post-transfer attached to the dermis. We conclude that this synthetic carrier membrane is a promising approach for delivery of therapeutic MSCs and opens the way for future studies to evaluate its impact on repairing difficult skin wounds.

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Section: Discussionmentioning

confidence: 99%

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Walker

Mistry²,

Smith³

et al. 2012

Tissue Engineering Part C: Methods

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“…Although a promising alternative, this culture does not maintain an expansion pattern similar to that obtained in cultures supplemented with serum [26,27]. PL thus far can be considered the most used supplement for GMP-compliant MSC expansion instead of FBS.…”

Section: Discussionmentioning

confidence: 99%

“…Another approach described in the literature involves the use of chemically defined serum-free media for MSC culture [26][27][28][29], therefore reducing batch-to-batch variability. Although a promising alternative, this culture does not maintain an expansion pattern similar to that obtained in cultures supplemented with serum [26,27].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion

Santos

Mizukami

Orellana

et al. 2016

Transfus Med Hemother

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Background: So far, using human blood-derived components appears to be the most efficient and safest approach available for mesenchymal stromal cell (MSC) expansion. In this paper, we report on the characterization of human AB serum (AB HS) produced by using different plasma sources, and its use as an alternative supplement to MSC expansion. Methods: Two plasma sources were used for AB HS production: plasma removed from whole blood after 24 h of collection (PC > 24 h) and plasma, cryoprecipitate reduced (PCryoR). The biochemical profile and quality of the produced AB HS batches were analyzed and their ability to support MSC cell growth after different storage times (0, 3, 6, 9 and 12 months) was evaluated. Results: The two plasma sources used showed similar characteristics regarding biochemical constituents and quality parameters and were effective in promoting MSC growth. MSCs cultured in medium supplemented with 10% AB HS presented similar doubling times and cumulative population doublings when compared to the 10% fetal bovine serum(FBS)-supplemented culture while maintaining immunophenotype, functional features, and cytogenetic profile. Conclusion: Overall, the results indicate that AB HS is an efficient FBS substitute and can be used for at least 12 months after production without impairing cell proliferation and quality.

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“…Outra abordagem descrita na literatura envolve o cultivo das CMM em meios isentos de soro (AGATA et al, 2009;RODRIGUES, GRIFFITH, WELLS, 2010;SOLMESKY et al, 2010;MIMURA et al, 2011;CHASE et al, 2012), os quais são compostos por suplementos sintéticos, reduzindo a variabilidade lote a lote. Apesar de ser uma alternativa promissora, as CMMs não mantêm um padrão de expansão semelhante ao obtido nas células em culturas suplementadas com soro (AGATA et al, 2009;RODRIGUES, GRIFFITH, WELLS, 2010).…”

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“…Apesar de ser uma alternativa promissora, as CMMs não mantêm um padrão de expansão semelhante ao obtido nas células em culturas suplementadas com soro (AGATA et al, 2009;RODRIGUES, GRIFFITH, WELLS, 2010).…”

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Desenvolvimento de metodologia para produção de soro AB humano para suplementação de meio de cultura destinado ao cultivo de células mesenquimais

Santos¹

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Feasibility and efficacy of bone tissue engineering using human bone marrow stromal cells cultivated in serum-free conditions

Cited by 78 publications

References 15 publications

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion

Desenvolvimento de metodologia para produção de soro AB humano para suplementação de meio de cultura destinado ao cultivo de células mesenquimais

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