Feasibility of a novel multispot nanoarray for antibiotic screening in honey

McNamee, Sara; Rosar, Giulia; Persic, Lidija; Elliott, Christopher T.; Campbell, Katrina

doi:10.1080/19440049.2017.1280188

Cited by 9 publications

(5 citation statements)

References 41 publications

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“…Moreover, the immunochromatographic test showed four nitrofuran metabolites in fish samples: 0.5 ppb (0.5 ng/mL) for AOZ and 0.75 ppb (0.75 ng/mL) for AHD, SEM, and AMOZ [30]. The detection of antibiotics in honey using a multispot nanoarray detected nitrofuran (AMOZ, AOZ, SEM, AHD) and chloramphenicol with detection limits of 0.23, 0.98, 24.8, 58.9 and 0.24 ppb (ng/mL), respectively [31]. On the other hand, it is known that the colorimetric method using AuNPs provides a rapid detection time within 5 min [32][33][34], which is similar to our system.…”

Section: Validation On Other Samples and Honeymentioning

confidence: 99%

Rapid Apta-Chromogenic Detection Method for Nitrofuran Metabolite Determination

Chaisri,

Jaengphop,

Hirono

et al. 2024

Molecules

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Nitrofuran (NF) contamination in food products is a global problem resulting in the banned utilization and importation of nitrofuran contaminated products. A novel chromogenic detection method using a specific DNA aptamer with high affinity and specificity to nitrofurans was developed. Single-stranded DNA aptamers specific to nitrofuran metabolites, including 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), and 1-aminohydantoin (AHD), were isolated using magnetic bead-SELEX. The colorimetric detection of nitrofurans using gold nanoparticles (AuNPs) exhibited an AOZ detection range of 0.01–0.06 ppb with a limit of detection (LOD) of 0.03 ppb. At the same time, this system could detect AMOZ and AHD at a range of 0.06 ppb and 10 ppb, respectively. The fast nitrofuran extraction method was optimized for food, such as fish tissues and honey, adjusted to be completed within 3–6 h. This novel apta-chromogenic detection method could detect NF metabolites with a sensitivity below the minimum required performance limit (MPRL). This analysis will be valuable for screening, with a shortened time of detection for aquaculture products such as shrimp and fish muscle tissues.

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Section: Validation On Other Samples and Honeymentioning

confidence: 99%

Rapid Apta-Chromogenic Detection Method for Nitrofuran Metabolite Determination

Chaisri,

Jaengphop,

Hirono

et al. 2024

Molecules

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“…A 200 μL aliquot of the overnight resulting culture was then inoculated into 10 ml of fresh SB medium containing ampicillin and incubated aerobically at 37°C and 220 rpm until the OD 600 reached approximately 0.6-0.8. The promoter was then induced by adding 0.6 mmol/L isopropyl β-D-thiogalactopyranoside (IPTG) and incubated for 7 h on a shaker at 28°C (Mcnamee et al, 2017). The medium after expression was centrifuged at 10,000 g for 10 min at 4°C, and the supernatant containing soluble fusion protein was collected.…”

Section: Expression and Identification Of The Soluble Anti-pcepamoz Scfv-ap Fusion Proteinmentioning

confidence: 99%

Development of a single-chain variable fragment-alkaline phosphatase fusion protein-based direct competitive enzyme-linked immunosorbent assay for furaltadone metabolite in ctenopharyngodon idellus

Chen

Hong

et al. 2021

Food and Agricultural Immunology

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To determine furaltadone metabolites 5-methylmorpholino-3amino-2-oxazolidinone (AMOZ) residual levels in ctenopharyngodon idellus, direct competitive enzyme-linked immunosorbent assay (dcELISA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. Firstly, V H and V L gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against AMOZ derivative, and then the productive V H and V L genes were assembled to a scFv gene and engineered to produce scFv-AP fusion protein. The fusion protein was further identified as a bifunctional reagent for immunoassay, then a sensitive one-step dcELISA against AMOZ derivative was developed with a halfmaximal inhibitory concentration (IC 50 ) and limit of detection (LOD) of 10.62 and 4.83 ng/mL, respectively. The recovery values of AMOZ from spiked fish samples determined by dcELISA were in 71.31%∼82.88%. The results suggested that the prepared scFv-AP fusion protein in this work can be used for rapid and convenient immunoassays to detect AMOZ residues in fish samples.

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“…Ongoing work by the current authors at Queen's University Belfast includes the development of a multiplex ELISA array for the simultaneous determination of AOZ, AMOZ, AHD, SEM and chloramphenicol. This platform has the ease of transfer and simplicity of use of current ELISA procedures with which the end user is already familiar [64]. A sciFLEXARRAYER S5 is used for spotting microwell plates and a sciReader CL colorimetric microarray reader used for scanning and analysing spot intensities.…”

Section: Multiplex Analysesmentioning

confidence: 99%

Development of Antibodies and Immunoassays for Monitoring of Nitrofuran Antibiotics in the Food Chain

Cooper

Fodey

Campbell

et al. 2018

COC

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Nitrofurans are a broad group of drugs once widely used for treatment of microbial and protozoal infections in many livestock species. However, as concerns grew globally with regard to the potentially carcinogenic and mutagenic effects of their residues in foods, they have been banned from use in many parts of the world. In order to monitor compliance to these bans it is essential to have fitfor-purpose testing methods. Immunoassays are the screening tool of choice for many testing laboratories due to their relative low cost, ease of use and high sensitivity. As is the case with all immunoassays, the most important reagents required are high quality, high affinity antibodies that exhibit the required sensitivity and specificity. Generating such antibodies for the nitrofuran family of compounds has required a great deal of effort in the design of immunogens, as the compounds, due to size, are not capable of eliciting an immune response in hosts and are not easily conjugated to carrier proteins. This article reviews the range of strategies used to successfully generate suitable antibodies to a wide range of these drugs and their metabolites. In addition, the platform technologies for nitrofuran detection have moved from simple enzyme-linked immunosorbent assay (ELISA)-based procedures to more sophisticated multiplexing systems which can undertake faster and broader spectrum testing for the parent drugs and their metabolites. Reviews of the technologies used for immunochemical detection of the nitrofurans and of commercially available test kits are also presented.

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Feasibility of a novel multispot nanoarray for antibiotic screening in honey

Cited by 9 publications

References 41 publications

Rapid Apta-Chromogenic Detection Method for Nitrofuran Metabolite Determination

Rapid Apta-Chromogenic Detection Method for Nitrofuran Metabolite Determination

Development of a single-chain variable fragment-alkaline phosphatase fusion protein-based direct competitive enzyme-linked immunosorbent assay for furaltadone metabolite in ctenopharyngodon idellus

Development of Antibodies and Immunoassays for Monitoring of Nitrofuran Antibiotics in the Food Chain

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