Feasibility of continuous glutamate monitoring in perfused retinal tissue with a potentiometric biosensing probe

Diaz, Gilda V.; El-Issa, Lina H.; Arnold, Mark A.; Miller, Robert F.

doi:10.1016/0165-0270(88)90023-4

Cited by 10 publications

(1 citation statement)

References 8 publications

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“…Various schemes for measuring glutamate based on enzymatic assay have been developed [59][60][61][62][63][64][65][66][67]. With the utilization of glutamate dehydrogenase, coupled with cosubstrate NAD + , the product of the reaction, NADH, can be monitored either by fluorescence or by absorption.…”

confidence: 99%

Selective detection of neurotransmitters by fluorescence and chemiluminescence imaging

Wang

Yeung

2001

Pure and Applied Chemistry

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In recent years, luminescence imaging has been widely employed in neurochemical analysis. It has a number of advantages for the study of neuronal and other biological cells: (1) a particular molecular species or cellular constituent can be selectively visualized in the presence of a large excess of other species in a heterogeneous environment; (2) low concentration detection limits can be achieved because of the inherent sensitivity associated with fluorescence and chemiluminescence; (3) low excitation intensities can be used so that long-term observation can be realized while the viability of the specimen is preserved; and (4) excellent spatial resolution can be obtained with the light microscope so subcellular compartments can be identified. With good sensitivity, temporal and spatial resolution, the flux of ions and molecules and the distribution and dynamics of intracellular species can be measured in real time with specific luminescence probes, substrates, or with native fluorescence. A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with CCD imaging is down to µM levels of glutamate with reasonable response time. We also found that chemiluminescence associated with the ATP-dependent reaction between luciferase and luciferin can be used to image ATP at levels down to 10 nM in the millisecond time scale. Similar imaging experiments should be feasible in a broad spectrum of biological systems.

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confidence: 99%

Selective detection of neurotransmitters by fluorescence and chemiluminescence imaging

Wang

Yeung

2001

Pure and Applied Chemistry

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Electrochemistry in Bioanalysis

Mikkelsen

2002

Encyclopedia of Electrochemistry

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The sections in this article are Introduction Bioanalysis Electroanalytical Methods Potentiometric Methods Introduction Zero‐Current Potentiometry ISEs in Bioanalysis Enzymes, Antibodies, and Nucleic Acids with Zero‐Current Potentiometry Dynamic Potentiometry Emerging Technology Commercialization of Potentiometric Devices Amperometric Methods Introduction Direct Amperometric Methods Amperometric Measurements with Enzymes Amperometric Immunoassays and Immunosensors Amperometric Nucleic Acid Assays and Sensors Emerging Technology Commercialization of Amperometric Devices Impedimetric Methods Introduction Impedimetric Assays and Sensors Commercially Available Instruments for Impedance Measurements Future Directions and Perspectives Acknowledgments

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Determination of glutamic acid decarboxylase activity and inhibition by an H2O2-sensing glutamic acid oxidase biosensor

Botrê

Botrè

Galli

et al. 1992

Analytical Biochemistry

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Feasibility of continuous glutamate monitoring in perfused retinal tissue with a potentiometric biosensing probe

Cited by 10 publications

References 8 publications

Selective detection of neurotransmitters by fluorescence and chemiluminescence imaging

Selective detection of neurotransmitters by fluorescence and chemiluminescence imaging

Electrochemistry in Bioanalysis

Determination of glutamic acid decarboxylase activity and inhibition by an H2O2-sensing glutamic acid oxidase biosensor

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