Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori, Hidemi; Ishihara, Masayuki

doi:10.3892/etm.2017.4041

Cited by 27 publications

(24 citation statements)

References 40 publications

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“…platelet-rich plasma (prp) is blood plasma with a significant amount of platelets that release the growth factors for wound healing and tissue regeneration. prp preparation is a procedure with minimal technical costs, and extensive use in stimulating tissue regeneration in different areas of medicine (9). activated platelets release more than 300 active agents from their intracellular granules, contain many cytokines, mitogens, anti-inflammatory factors, and other bioactive molecules, which are essential regulators in a complicated microenvironment of the regenerative process, which enhance the healing process.…”

Section: Discussionmentioning

confidence: 99%

“…platelet-rich plasma became widely used in clinical practice because a high concentration of growth factors should promote better tissue regeneration (4,13,17). Despite a large number of studies devoted to PRP effect on intestinal anastomosis regeneration, still, the impact of autoplasma on adhesion and different methods of PRP application is not well established (5,9). as a rule, soaking the intestinal walls with prp precedes the anastomosis formation (13,14), although injected PRP, which proved to be highly effective in other areas of medicine (17,18,19,20), has not been applied to the intestinal anastomosis.…”

Section: Introductionmentioning

confidence: 99%

“…Many experimental studies dealing with the strength of the formed intestinal anastomoses define wound-bursting strength at an increased intestinal pressure (5,9,13,14). We believe the anastomosis regeneration indicators should be extend-ed to measuring the intestinal anastomosis strength with morphometric indicators (21,22).…”

Section: Introductionmentioning

confidence: 99%

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Platelet-rich autoplazma effect on intestinal anastomosis regeneration in rabbits

Nuraddinovich-Dzhumabekov

Tulegenovich-Dzhumabekov

Kazybaevich-Ismailov

et al. 2020

lib oncol

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Background: the failure of the intestinal anastomosis remains one of the main problems in gastrointestinal surgery. new principles for solving this problem are focused on the cellular level of regeneration using platelet-rich plasma (prp). In this study, we evaluated the effect of PRP and experimentally compared the effectiveness of various application methods in intestinal anastomosis regeneration in rabbits. Materials and Methods: platelet-rich autoplasma was obtained from 81 rabbits, which we used to study two methods of applying prp (injection into the muscle layer/soaking in the intestinal wall) in comparison with the control group on the end-to-end small-intestinal anastomosis. We performed a morphological study of intestinal anastomoses after 24, 48, 72 hours, and after 7 days. We evaluated the deformation and strength characteristics of the anastomosis on the 7th day. Results: analysis of the histological and morphometric data of the study showed that the regeneration between the samples of intestinal anastomosis treated with prp soaking and injections into the intestinal wall is more favorable in comparison with the control group. The criteria were the quantitative ratio of inflammatory infiltrate, fibroblast proliferation, neoangiogenesis, and collagen deposition. PRP soaking in the intestinal wall significantly increased the number of adhesion conglomerates, which contributed to severe deformation and narrowing of more than ½ of the lumen in the intestinal tube. Analysis of the deformation and strength characteristics of the formed anastomoses also showed significantly higher values of the rupture strength of the anastomoses treated with prp, in contrast to the control group. Conclusion: PRP soaking and injection into the intestinal wall can have a positive effect on the regeneration of the intestinal anastomosis. PRP injection into the muscular layer of the intestinal wall significantly reduces the development of adhesions in comparison with the prp-saturated group of intestinal anastomoses. further research is needed to clarify the optimal method of prp application, which will improve the regeneration of the intestinal anastomosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet-rich autoplazma effect on intestinal anastomosis regeneration in rabbits

Nuraddinovich-Dzhumabekov

Tulegenovich-Dzhumabekov

Kazybaevich-Ismailov

et al. 2020

lib oncol

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“…Nowadays, platelet-rich plasma is widely used in clinical practices, because high concentration of growth factors should promote better tissue regeneration [4][5][6] . Despite a large number of studies devoted to PRP effect on intestinal anastomosis regeneration, still the impact of auto-plasma on adhesion and different methods of PRP application are underdeveloped 7,8 . As a rule, soaking the intestinal walls with PRP is followed by anastomosis 5,9 , although injected PRP, which proved to be highly effective in other areas of medicine 5,[10][11][12] , has not been applied to intestinal anastomosis.…”

Section: Introductionmentioning

confidence: 99%

Platelet-rich autoplasma effect on intestinal anastomosis regeneration in rabbits

Dzhumabekov¹,

Джумабеков²,

ISMAILOV³

et al. 2019

Arch Balk Med Union

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L'effet de l'auto-plasma riche en plaquettes sur la régénération de l'anastomose intestinale chez le lapin Introduction. La régénération de la plaie est un processus important dans les pratiques chirurgicales. Cependant, l'échec d'une anastomose intestinale reste un problème de chirurgie intestinale. L'objectif de l'étude. Évaluer l'effet du plasma riche en plaquettes (PRP) et comparer l'efficacité de différentes méthodes d'application dans la régénération de l'anastomose intestinale chez le lapin. Méthodes. Un auto-plasma riche en plaquettes a été obtenu chez 81 lapins, qui ont été utilisés pour étudier deux méthodes d'application de PRP (injection dans la couche musculaire / immersion dans la paroi intestinale) par rapport au groupe témoin sur l'anastomose de bout en bout de l'intestin grêle.. Résultats. L'analyse des données histologiques et morphométriques de l'étude a montré que la régénération entre les échantillons d'anastomose intestinale traités avec du trempage au PRP et les injections dans la ABSTRACT Introduction. Wound regeneration is an important process in surgical interventions. Failure of an intestinal anastomosis is an important intestinal surgery problem. The objective of the study. To evaluate the effect of platelet-rich plasma (PRP) and compare the effectiveness of various application methods in intestinal anastomosis regeneration in rabbits. Methods. Platelet-rich auto-plasma was obtained from 81 rabbits, which was used to study two methods of applying PRP (injection into the muscle layer / soaking in the intestinal wall) in comparison with the control group, on the end-to-end small-intestinal anastomosis. Results. The analysis of the histological and morphometric data showed that the regeneration between the samples of intestinal anastomosis treated with PRP soaking and injections into the intestinal wall is more favorable in comparison with the control group, which, in turn, was determined by the quantitative ratio of inflammatory infiltrate, fibroblast proliferation, neo-angiogenesis, and collagen deposition. PRP

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“…Furthermore, chitosan has biological properties such as hemostatic activity, antibacterial activity, and ability to accelerate wound healing 8 . Thereby, it is being used in many medical devices and health-care products 9 . Recently, several reports have shown the usefulness and effectiveness of chitosan for postpartum hemorrhage 10,11 .…”

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confidence: 99%

Efficacy of a chitosan tampon in the loop electrosurgical excision procedure: A prospective randomized controlled study

Chong

Lee

Jeon

et al. 2020

Sci Rep

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It has been reported that chitosan has a hemostatic effect and an antibiotic activity. This study aimed to evaluate the efficacy and feasibility of using a chitosan tampon (Hemoblock-Tampon) in preventing hemorrhage and enhancing wound healing after the loop electrosurgical excision procedure (LEEP).This single-blind, prospective, randomized study included 62 consecutive patients who underwent LEEP for cervical intraepithelial neoplasia. A chitosan tampon (31 patients; treatment group), or a general tampon (31 patients; control group) was applied to the uterine cervix immediately after LEEP. One patient in the treatment group declined to participate in this study. Thus, 30 patients in the treatment group and 31 patients in the control group completed this study. For objective analysis of hemorrhage in the postoperative 2 weeks, the amounts of bleeding were checked daily with a pictorial blood assessment chart. We evaluated vaginal discharge, abdominal pain, and impairment in daily living during the postoperative 2 weeks using 5 visual analogue scale questionnaires.The bleeding count was significantly lower in the treatment group than in the control group (21.37 ± 16.86 vs. 40.52 ± 16.55, p = 0.0014). The sum of the scores of the 5 questionnaires was significantly lower in the treatment group than in the control group (6.53 ± 2.84 vs. 8.59 ± 2.88, p = 0.0079). The incidence of vaginal discharge was significantly lower in the treatment group than in the control group (20.0% vs. 48.4%, p = 0.0207). According to logistic regression, only the use of chitosan tampon reduced the risk of moderate to severe vaginal bleeding 2 weeks after surgery (Odd ratio, 0.213; 95% confidence interval, 0.06-0.76; p = 0.0172). Complete healing of the uterine cervix occurred in 86.7% of patients in the treatment group and in 61.3% of patients in the control group at 4 weeks after surgery (p = 0.0255).The use of chitosan tampons can reduce hemorrhage, vaginal discharge, abdominal pain, and impairment of daily living after LEEP. Moreover, chitosan tampon may help enhance wound healing.

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Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Cited by 27 publications

References 40 publications

Platelet-rich autoplazma effect on intestinal anastomosis regeneration in rabbits

Platelet-rich autoplazma effect on intestinal anastomosis regeneration in rabbits

Platelet-rich autoplasma effect on intestinal anastomosis regeneration in rabbits

Efficacy of a chitosan tampon in the loop electrosurgical excision procedure: A prospective randomized controlled study

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