Feasibility of Monte CarlO simulations in Quantitative Tissue Imaging

Maeder, Ulf; Schmidts, Thomas; Avci, Ersin; Heverhagen, Johannes T.; Runkel, Frank; Fiebich, Martin

doi:10.1177/039139881003300410

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…Accompanying these studies, Monte Carlo models are designed to calculate calibration factors for quantitative measurements [8]. Using these factors it becomes possible to convert the detected signal intensities into the dye concentration.…”

Section: Discussionmentioning

confidence: 99%

4D confocal microscopy method for drug localization in the skin

et al. 2011

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A 4D confocal microscopy (xyzλ) method for measuring the drug distribution in skin samples after a permeation study is investigated. This approach can be applied to compare different drug carrier systems in pharmaceutical research studies.For the development of this detection scheme phantom permeation studies and preliminary skin measurements are carried out. The phantom studies are used to detect the permeation depth and the localization of the external applied fluorescent dye naphthofluorescein that is used as a model agent. The skin study shows the feasibility of the method for real tissue.For the differentiation of tissue/phantom and the dye, spectral unmixing is performed using the spectral information detected by a confocal microscope. The results show that it is possible to identify and localize external dyes in the phantoms as well as in the skin samples.

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Section: Discussionmentioning

confidence: 99%

4D confocal microscopy method for drug localization in the skin

et al. 2011

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“…La viabilidad del método de MC puede encontrarse en la bibliografía así como la del programa comercial 22 . Además los autores han validado igualmente el programa comercial 23 estudiando las diferencias en reflectancias y transmitancias de varios tejidos adiposos y del corazón encontrando minúsculas diferencias entre el programa y otras referencias.…”

Section: Figura 2 Piel Simulada Simulación De Un Tejido Epitelial Cunclassified

Estudio y caracterización óptica de un Carcinoma Basocelular mediante simulación láser numérica

Fernández¹,

Alcarria

González‐Marcos

et al. 2015

Sanid. Mil.

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RESUMEN Antecedentes: Las simulaciones de Monte Carlo son una de las técnicas más utilizadas en el contexto de modelación de geometrías de sistemas heterogéneos, como puede ser la piel humana. Además uno de los principales retos modernos en biofotónica es la simulación del comportamiento óptico y la propagación de la luz en tejidos. Objetivos: Analizar la respuesta óptica de la piel, y aportar información para el diagnóstico de posibles alteraciones en la piel con la presencia de un basalioma. Material y Método: Un programa comercial, contrastado y fiable de simulación basado en técnicas de Monte Carlo, que permite estudiar el comportamiento de la piel frente a una radiación óptica; la aproximación a la trayectoria que sigue la luz en su interacción con el tejido se obtiene a partir de las propiedades ópticas conocidas a priori. Piel modelada y simulación numérica. Resultados: Los resultados expuestos son datos innovadores, hasta donde conocen los autores, sobre patrones de radiación en unidades de irradiancia como de reflectividad y transmitividad de la piel. Conclusiones: Este trabajo ofrece un método para distinguir entre piel «sana» y piel con carcinoma basocelular, lo que podría facilitar un procedimiento de identificación de lesiones cancerosas de la piel. PALABRAS CLAVE: Simulación Monte Carlo, fotones, propiedades ópticas, piel, basalioma. Study and optical characterization of a Basal Cell Carcinoma by numerical laser simulation SUMMARY: Background: Monte Carlo simulations are one of the most used techniques in the context of modeling geometry such as human skin. One of the main challenges in biophotoncs is the simulation of light spread in biological tissues. Objectives: to analyze the optical response of the skin, and to contribute information for the diagnosis and treatment of possible alteration in the skin. Material and methods: A commercial, proven and reliable simulation program based on Monte Carlo techniques, which allow studying the behavior of the skin from optical radiation. The approximation to the path followed by the light in its interaction with the tissue is obtained from known-optical properties. Modeling and numerical simulation of skin. Results: The results presented are innovative data about radiation patterns in units of irradiance as reflectivity and transmissivity of the skin. Conclusions: This work provides a method to distinguish between "healthy" skin and skin with basal cell carcinoma, which could provide a method for identifying skin cancer, allowing state that can later be extrapolated to other types of cancers and skin diseases.

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“…In these cases intensity-based methods can be used that need to be calibrated or intensity corrected. Monte Carlo simulations that determine and compensate for effects of signal attenuation due to scattering and absorption 32 are often applied for these applications. Combining these techniques with the spectral evaluation for penetration studies would add valuable information to the localization and penetration depth of dye and agents.…”

Section: Advantages and Drawbacks For Delivery Studiesmentioning

confidence: 99%

Evaluation and quantification of spectral information in tissue by confocal microscopy

Maeder¹,

Marquardt²,

Beer³

et al. 2012

J. Biomed. Opt

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Abstract. A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The method is tested and validated in the context of the development of dermal drug delivery systems, introducing a quantitative uptake indicator to compare the performances of different delivery systems is introduced. A drug penetration study was performed in vitro. The results show that the method is able to detect, visualize and measure spectral information in tissue. In the penetration study, uptake efficiencies of different experiment setups could be discriminated and quantitatively described. The developed uptake indicator is a step towards a quantitative assessment and, in a more general view apart from pharmaceutical research, provides valuable information on tissue composition. It can potentially be used for clinical in vitro and in vivo applications.

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Feasibility of Monte CarlO simulations in Quantitative Tissue Imaging

Cited by 4 publications

References 17 publications

4D confocal microscopy method for drug localization in the skin

4D confocal microscopy method for drug localization in the skin

Estudio y caracterización óptica de un Carcinoma Basocelular mediante simulación láser numérica

Evaluation and quantification of spectral information in tissue by confocal microscopy

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