Feasibility of Parylene Coating for Planar Electroporation Copper Electrodes

Abstract

This paper is focused on the feasibility study of parylene as a biocompatible coating for planar electroporation microelectrodes. The planar parallel and the circular interdigitated electrodes are applied in the analysis. The electrodes feature 100 μm width with a 300 μm gap between anode and cathode. The parylene coating thickness was varied in the 250 nm-2 μm range. The resultant electric field distribution evaluation has been performed using the finite element method. The electrodes have been applied in electroporation experiments with Saprolegnia parasitica. For reference the additional experiments using conventional electroporation cuvette (1 mm gap) have been performed. It has been determined that the parylene coating with hydrophobic properties has limited applicability for the passivation of the planar electroporation electrodes.

Research and development of the high-frequency square-wave pulse electroporation system

“…The planar electrode structures proved to be advantageous for this type electroporation experiments (Geng et al 2013;Novickij, Tabašnikov, et al 2017), but various issues, such as the spatial non-uniformity of the electric field distribution, complexity of electrode fabrication, biological compatibility issues, and the voltage breakdown occurrence between the electrodes has to be solved (Novickij, Tabašnikov, et al 2017). Due to this, the variety of available planar electrodes for electroporation is currently limited due to the complexity, price and the electrodes fabrication challenges.…”

Validation of a SPICE Model for High Frequency Electroporation Systems

“…However, further studies have reported that square-wave pulses are more efficient during the application of high intensity (tens to hundreds of kV/cm) nanosecond PEFs, due to its enhanced control of the pulse energy [33]. This is an important aspect for the rapidly growing microfluidics-based electroporation technique [34], wherein planar electrode structures have proved to be advantageous [34,35].…”

Development of Planar Electrodes for Real-Time Electroporation / Planariųjų Elektrodų Modelis Realiojo Laiko Elektroporacijos Tyrimams

“…The planar electrode structures proved to be advantageous for this type electroporation experiments (Geng & Lu, 2013;Novickij et al, 2017), but various issues, such as the spatial non-uniformity of the electric field distribution, complexity of electrode fabrication, biological compatibility issues, and the voltage breakdown occurrence between the electrodes has to be solved (Novickij et al, 2017). Due to this, the variety of available planar electrodes for electroporation is currently limited due to the complexity, price and the electrodes fabrication challenges.…”