Feasibility of Phospholipids Separation by Packed Column SFC with Mass Spectrometric and Light Scattering Detection

Yip, Henry; Ashraf‐Khorassani, Mehdi; Taylor, Larry T.

doi:10.1365/s10337-007-0235-5

Cited by 28 publications

(9 citation statements)

References 28 publications

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“…The goal of our study was to fractionate the individual PLs inherent to crude soybean lecithin and to assay the extracted fractions by a method that is described in a recent publication via supercritical fluid chromatography with evaporative light scattering and mass spectrometric detection [24]. In order to achieve this goal, neutral, less polar lipids had to be removed.…”

Section: Resultsmentioning

confidence: 99%

Analytical scale supercritical fluid fractionation and identification of single polar lipids from deoiled soybean lecithin

Yip

Ashraf‐Khorassani

Taylor

2008

J of Separation Science

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The phosphatidylcholine (PC)-enriched fraction from soybean lecithin is of interest due to its critical role in both the pharmaceutical and industrial field. In this work, enhancement of the purity of the PC fraction along with other individual polar lipid fractions was achieved from crude soybean lecithin by using supercritical fluid extraction (SFE) with methanol-modified SC-CO(2). Neutral lipids were first removed from the crude sample using pure CO(2). Then, the effect of CO(2 )pressure, temperature, and modifier percentage on phospholipid (PL) fractionation from deoiled lecithin was compared with and without silica gel mixed with the lecithin. Pure fractions of phosphatidylethanolamine (PE) and PC were obtained by varying the modifier concentration of the extraction fluid at 460 atm and 40 degrees C with silica gel added to the deoiled lecithin. Without silica gel, coextraction of PE and PC was observed. A total of six components were isolated and tentatively identified in the extract of deoiled crude soybean lecithin.

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Section: Resultsmentioning

confidence: 99%

Analytical scale supercritical fluid fractionation and identification of single polar lipids from deoiled soybean lecithin

Yip

Ashraf‐Khorassani

Taylor

2008

J of Separation Science

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“…By using supercritical fluids with low viscosity and high diffusivity, such as supercritical carbon dioxide (scCO 2 ), as mobile phase, SFC exhibits some interesting features [12], such as high separation efficiency, high flow-rates and thus reduced analysis times. Beside the successful achievements in chiral separations, SFC has also showed great potential in the separation and isolation of active components in TCMs and natural products, such as lipids [13], vitamins [14], ginkgolides [15], triterpenoids [16], regioisomeric spirostanol saponin diastereomers [17]. However, very few applications have been focused on the separation of TSs using SFC [18,19].…”

Section: Introductionmentioning

confidence: 99%

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Huang

Zhang

Zhou

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tTriterpenoid saponins (TSs) are the most important components of some traditional Chinese medicines (TCMs) and have exhibited valuable pharmacological properties. In this study, a rapid and efficient method was developed for the separation of kudinosides, stauntosides and ginsenosides using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The separation conditions for the selected TSs were carefully optimized after the initial screening of eight stationary phases. The best compromise for all compounds in terms of chromatographic performance and MS sensitivity was obtained when water (5-10%) and formic acid (0.05%) were added to the supercritical carbon dioxide/MeOH mobile phase. Beside the composition of the mobile phase, the nature of the make-up solvent for interfacing SFC with MS was also evaluated. Compared to reversed phase liquid chromatography, the SFC approach showed higher resolution and shorter running time. The developed SFC-MS methods were successfully applied to the separation and identification of TSs present in Ilex latifolia Thunb., Panax quinquefolius L. and Panax ginseng C.A. Meyer. These results suggest that this SFC-MS approach could be employed as a useful tool for the quality assessment of natural products containing TSs as active components.

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“…Impregnation of polymer can be achieved either by deposition or molecular dispersion phenomenon [103,104] Masmoodi et al [105] were carried out by using common stationary phase (Chiralcel OD and AD, Chirobiotc V and T), CO 2 modified with 5-30 % of methanol [110]. Liu et al [111] performed the enantiomeric separation of macrocyclic glycopeptide (teicoplanin) and some of its common derivatives, (b) Separation of achiral compounds such as the separation of mixture of estrogen metabolites [112] and separation of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine by SFC coupled with light scattering and mass spectrometric detection [113], (c) analysis of peptidesamong the peptides separated and analyzed by SFC are cyclosporin [114], actinomycin D and vancomycin [108], gramycidin A, B, and C [115], (d) Coupling of SFC with mass spectroscopy, which helps to obtained good peak shape and signal in chromatogram [116]. A large number of drugs and materials used for various SCF techniques are listed in Table. 2.…”

Section: Impregnationmentioning

confidence: 99%

Supercritical Fluid Technology: A Review

Parhi¹,

Suresh²

2013

JAPST

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The challenges ever faced by pharmaceutical industry is mainly due to discovery of new drugs and development of new technologies. Supercritical fluid (SCF) technology is one such technique, which has become an important tool in the production of different particulate systems along with extraction and drying of protein and peptides during last couple of decade because of its specific properties such as flexibility in use, reduced environmental concern and its simplicity. In this review, we briefly describe the operating principles and parameters influencing each one of SCF processes along with their merits and perspectives. The application of SCF technology in pharmaceutical industry, including particle and crystal engineering, composite particles' preparation, coating of solid dosage form, liposome preparation, extraction and protein and peptide drying are discussed.

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Feasibility of Phospholipids Separation by Packed Column SFC with Mass Spectrometric and Light Scattering Detection

Cited by 28 publications

References 28 publications

Analytical scale supercritical fluid fractionation and identification of single polar lipids from deoiled soybean lecithin

Analytical scale supercritical fluid fractionation and identification of single polar lipids from deoiled soybean lecithin

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Supercritical Fluid Technology: A Review

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