Feasibility of speckle variance OCT for imaging cutaneous microvasculature regeneration during healing of wounds in diabetic mice

Sharma, Priyanka; Kumawat, Jyoti; Kumar, Saurabh; Sahu, Khageswar; Verma, Yogesh Kumar; Gupta, Pradeep Kumar; Rao, K. Divakar

doi:10.1088/1555-6611/aa995f

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…Speckle-variance-OCT (SV-OCT) has the capability of depth-resolved 3D imaging, typically requiring just seconds to acquire (Mariampillai et al 2008, Enfield et al 2011. As mentioned, the speckle pattern is dependent upon the spatial and temporal fluctuations in the intensity of light being backscattered from the tissue being imaged (Sharma et al 2018). Contrasting the vasculature within such relies on the statistical analysis of the interframe speckle variance being generated by moving red blood cells (Son et al 2016).…”

Section: Dynamic Optical Coherence Tomography (Doct)mentioning

confidence: 99%

Microvascular imaging of the skin

Deegan

Wang

2019

Phys. Med. Biol.

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Despite our understanding that the microvasculature plays a multifaceted role in the development and progression of various conditions, we know little about the extent of this involvement. A need exists for non-invasive, clinically meaningful imaging modalities capable of elucidating microvascular information to aid in our understanding of disease, and to aid in the diagnosis/monitoring of disease for more patient-specific care. In this review article, a number of imaging techniques are summarized that have been utilized to investigate the microvasculature of skin, along with their advantages, disadvantages and future perspectives in preclinical and clinical settings. These techniques include dermoscopy, capillaroscopy, Doppler sonography, laser Doppler flowmetry (LDF) and perfusion imaging, laser speckle contrast imaging (LSCI), optical coherence tomography (OCT), including its Doppler and dynamic variant and the more recently developed OCT angiography (OCTA), photoacoustic imaging, and spatial frequency domain imaging (SFDI). Attention is largely, but not exclusively, placed on optical imaging modalities that use intrinsic optical signals to contrast the microvasculature. We conclude that whilst each imaging modality has been successful in filling a particular niche, there is no one, all-encompassing modality without inherent flaws. Therefore, the future of cutaneous microvascular imaging may lie in utilizing a multi-modal approach that will counter the disadvantages of individual systems to synergistically augment our imaging capabilities.

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Section: Dynamic Optical Coherence Tomography (Doct)mentioning

confidence: 99%

Microvascular imaging of the skin

Deegan

Wang

2019

Phys. Med. Biol.

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“…However, no OCTA studies for the diseased fish, especially zebrafish in diabetes, have been reported yet. This work was the first adult zebrafish study to demonstrate the adverse effect of the diabetic disease on wound healing, although the effect has been well documented by the previous OCT/OCTA studies of the animal diabetes models [46,52,53].…”

Section: Discussionmentioning

confidence: 96%

Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography

Kim

Choi

2023

Bioengineering

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A diabetic wound presents a severe risk of infections and other complications because of its slow healing. Evaluating the pathophysiology during wound healing is imperative for wound care, necessitating a proper diabetic wound model and assay for monitoring. The adult zebrafish is a rapid and robust model for studying human cutaneous wound healing because of its fecundity and high similarities to human wound repair. OCTA as an assay can provide three-dimensional (3D) imaging of the tissue structure and vasculature in the epidermis, enabling monitoring of the pathophysiologic alterations in the zebrafish skin wound. We present a longitudinal study for assessing the cutaneous wound healing of the diabetic adult zebrafish model using OCTA, which is of importance for the diabetes research using the alternative animal models. We used non-diabetic (n = 9) and type 1 diabetes mellitus (DM) adult zebrafish models (n = 9). The full-thickness wound was generated on the fish skin, and the wound healing was monitored with OCTA for 15 days. The OCTA results demonstrated significant differences between diabetic and non-diabetic wound healing, involving delayed tissue remodeling and impaired angiogenesis for the diabetic wound, leading to slow wound recovery. The adult zebrafish model and OCTA technique may benefit long-term metabolic disease studies using zebrafish for drug development.

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Quantitative Phase Optical Microscopic Techniques for Biomedical Imaging and Diagnostic Applications

Mehta

2018

Proc. Natl. Acad. Sci., India, Sect. A Phys. Sci.

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Feasibility of speckle variance OCT for imaging cutaneous microvasculature regeneration during healing of wounds in diabetic mice

Cited by 5 publications

References 24 publications

Microvascular imaging of the skin

Microvascular imaging of the skin

Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography

Quantitative Phase Optical Microscopic Techniques for Biomedical Imaging and Diagnostic Applications

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