The development of the bioethanol industry is facing
a bottleneck
due to challenges in the collection and storage of biomass. In this
study, we explored the use of compacted silage fermentation to preserve
whole pulverized sweet sorghum. To sterilize native and contaminant
bacteria, we employed sulfur dioxide and inoculated SO2-tolerant Saccharomyces cerevisiae TSH4 for long-term preservation in an ethanol atmosphere. Bagged
silage experiments present the most effective preservation dosage,
which was 2000 ppm SO2 concentration and 2% TSH4 yeast
inoculation. Based on these conditions, we found that compacted silage
with different compaction densities enabled all fermentations to be
completed within 60 days. The highest ethanol concentration is achieved
at a compaction density of 600, at which ethanol conversion rate reaches
to 93.4%. Higher compaction densities produce more leachate, which
results in ethanol loss up to 9.7%. During the 240 days of long-term
storage, there was no significant change in ethanol content despite
an increase in acetic acid concentration. Additionally, distilled
vinasse was found to be comparable to whole silage maize in terms
of nutrients, thus proving the feasibility of constructing distributed
biofuel plants.