Features of biomechanical properties of human coronary arteries

Kasjanovs, Vladimirs; Ozolanta, Iveta; Puriņa, Brigita

doi:10.1007/bf02257246

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…When comparing the rhomboid constructs to the porcine coronary arteries, the 70° (Blood Vessel 1: 72.4 kPa/70°: 75.5 kPa) and 34° (Blood Vessel 2: 38.7 kPa/34°: 37.3 kPa) VolMEW constructs showed a good approximation of the maximum stress levels, while the 34° rhomboid reinforced constructs also showed a comparable overall curve trajectory to the physiological specimens (Figure 3F). Due to inter‐patient variability and the wide range of mechanical properties that vessels display even when taken from adjacent anatomical locations, [ 50 ] these results underscore the versatility of the proposed VolMEW system to modulate the mechanical profile of the printed composite tubes in order to approximate physiological blood vessel mechanics and to utilize the mesh design to account for natural variation. In summary, the presence of the MEW scaffolds endows the otherwise mechanically weak hydrogel construct with superior mechanical properties, and stress‐deformation profiles approaching those displayed by native vessels.…”

Section: Resultsmentioning

confidence: 99%

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics

et al. 2023

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Major challenges in biofabrication revolve around capturing the complex, hierarchical composition of native tissues. However, individual 3D printing techniques have limited capacity to produce composite biomaterials with multi‐scale resolution. Volumetric bioprinting recently emerged as a paradigm‐shift in biofabrication. This ultrafast, light‐based technique sculpts cell‐laden hydrogel bioresins into 3D structures in a layerless fashion, providing enhanced design freedom over conventional bioprinting. However, it yields prints with low mechanical stability, since soft, cell‐friendly hydrogels are used. Herein, the possibility to converge volumetric bioprinting with melt electrowriting, which excels at patterning microfibers, is shown for the fabrication of tubular hydrogel‐based composites with enhanced mechanical behavior. Despite including non‐transparent melt electrowritten scaffolds in the volumetric printing process, high‐resolution bioprinted structures are successfully achieved. Tensile, burst, and bending mechanical properties of printed tubes are tuned altering the electrowritten mesh design, resulting in complex, multi‐material tubular constructs with customizable, anisotropic geometries that better mimic intricate biological tubular structures. As a proof‐of‐concept, engineered tubular structures are obtained by building trilayered cell‐laden vessels, and features (valves, branches, fenestrations) that can be rapidly printed using this hybrid approach. This multi‐technology convergence offers a new toolbox for manufacturing hierarchical and mechanically tunable multi‐material living structures.

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Section: Resultsmentioning

confidence: 99%

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics

et al. 2023

View full text Add to dashboard Cite

show abstract

“…When comparing the rhomboid constructs to the porcine coronary arteries, the 70° (Blood Vessel 1: 72.4 kPa / 70°: 75.5 kPa) and 34° (Blood Vessel 2: 38.7 kPa / 34°: 37.3 kPa) VolMEW constructs showed a good approximation of the maximum stress levels, while the 34° rhomboid reinforced constructs also showed a comparable overall curve trajectory to the physiological specimens (Figure 3F). Due to inter-patient variability and the wide range of mechanical properties that vessels display even when taken from adjacent anatomical locations, [46] these results underscore the versatility of the proposed VolMEW system to modulate the mechanical profile of the printed composite tubes in order to approximate physiological blood vessel mechanics and to utilize the mesh design to account for natural variation.…”

Section: Resultsmentioning

confidence: 99%

Volumetric Printing across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics

Groessbacher

Kopp

Gergely

et al. 2023

Preprint

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Major challenges in biofabrication revolve around capturing the complex, hierarchical composition of native tissues. However, individual 3D printing techniques have limited capacity to produce composite biomaterials with multi-scale resolution. Volumetric bioprinting recently emerged as a paradigm-shift in biofabrication. This ultra-fast, light-based technique sculpts cell-laden hydrogel bioresins into three-dimensional structures in a layerless fashion, providing unparalleled design freedom over conventional bioprinting. However, it yields prints with low mechanical stability, since soft, cell-friendly hydrogels are used. Herein, for the first time, the possibility to converge volumetric bioprinting with melt electrowriting, which excels at patterning microfibers, is shown for the fabrication of tubular hydrogel-based composites with enhanced mechanical behavior. Despite including non-transparent melt electrowritten scaffolds into the volumetric printing process, high-resolution bioprinted structures were successfully achieved. Tensile, burst and bending mechanical properties of printed tubes were tuned altering the electrowritten mesh design, resulting in complex, multi-material tubular constructs with customizable, anisotropic geometries that better mimic intricate biological tubular structures. As a proof-of-concept, engineered vessel-like structures were obtained by building tri-layered cell-laden vessels, and features (valves, branches, fenestrations) that could be resolved only by synergizing these printing methods. This multi-technology convergence offers a new toolbox for manufacturing hierarchical and mechanically tunable multi-material living structures.

show abstract

“…When comparing the rhomboid constructs to the porcine coronary arteries, the 70° (Blood Vessel 1: 72.4 kPa / 70°: 75.5 kPa) and 34° (Blood Vessel 2: 38.7 kPa / 34°: 37.3 kPa) VolMEW constructs showed a good approximation of the maximum stress levels, while the 34° rhomboid reinforced constructs also showed a comparable overall curve trajectory to the physiological specimens (Figure 3F). Due to inter-patient variability and the wide range of mechanical properties that vessels display even when taken from adjacent anatomical locations, [301] these results underscore the versatility of the proposed VolMEW system to modulate the mechanical profile of the printed composite tubes in order to approximate physiological blood vessel mechanics and to utilize the mesh design to account for natural variation. In summary, the presence of the MEW scaffolds endows the otherwise mechanically weak hydrogel construct with superior mechanical properties, and stress-deformation profiles approaching those displayed by native vessels.…”

Section: Resultsmentioning

confidence: 99%

Crafting Tissue Complexity

Núñez Bernal

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The global rise in life expectancy is accompanied by an increasing prevalence of chronic diseases, posing economic burdens and impacting patients' well-being. This surge in disease incidence challenges the drug discovery pipeline, calling for the adaptation of its rules and regulations to reduce the ever-increasing failure rates of new drugs. The staggering growth of the pharmaceutical industry in the last decades, and the high expenditure required to bring new drugs to the market is set to become an unsustainable issue in the coming years. Recently, far more attention has been focused on the preclinical phase of the pipeline, where drug evaluation culminates in animal testing, the current gold standard to ensure drug safety and efficacy before human trials. A consensus on the need for more complex and predictive human disease models has emerged and become a priority for scientists and policy makers in the past decade. Biofabrication, an emerging technology-driven field, has gained prominence in biomedical research for developing advanced in vitro models. Biofabrication is “the automated generation of biologically functional products with structural organization from living cells, bioactive molecules, biomaterials, cell aggregates such as micro-tissues, or hybrid cell-material constructs, through Bioprinting or Bioassembly and subsequent tissue maturation processes”. The last decades have seen the rapid development new bioprinting modalities, opening the door to the development of architecturally complex in vitro platforms where multi-cellular and multi-material structures can be more easily created. Considering the need for more complex preclinical models to bridge the translational gap between 2D in vitro models, animal testing and clinical trials, the overarching aim of this thesis was “to develop new biofabrication approaches, encompassing 3D bioprinting technologies, powerful biological building blocks, and smart biomaterials, that facilitate the development of advanced human in vitro models with native tissue-like functionality”. This research has introduced significant advancements within the field of biofabrication for the generation of human in vitro models. Through a comprehensive exploration that tackled challenges related to fundamental bioprinting principles, the biological intricacies, and the biochemical and material property requirements of bioprinted structures to better recapitulate native tissues, the developments described here have expanded the toolkit of biofabrication approaches. By introducing novel technologies, like the pioneering, layerless volumetric bioprinting strategy, and synergizing new and existing printing approaches to harness their unique advantages, this thesis showcases a variety of functional bioprinted tissue models that enable the study of biological processes in vitro. The thorough exploration of volumetric bioprinting, distinguished by its scalability, unparalleled design freedom, compatibility with advanced biological tools, and a growing library of smart materials, charts new paths toward the creation of clinically-relevant testing platforms. The bioprinted, tissue-specific in vitro models presented here offer enhanced physiological accuracy and predictability and hold the potential to incorporate patient-specific elements for personalized medicine. The toolkit developed in here represents a significant stride in bridging the translational gap of tissue-engineered in vitro models, particularly in the context of preclinical testing. All in all, the evolving landscape of biofabricated in vitro models holds promise for innovative approaches in the future.

show abstract

Features of biomechanical properties of human coronary arteries

Cited by 8 publications

References 24 publications

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics

Volumetric Printing across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics

Crafting Tissue Complexity

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