Features of Immune Response and Basophil Activation in Children With Chronic Urticaria

Синельникова, Н. А.; Бычкова, Н. В.; Калинина, Н. М.

doi:10.15789/1563-0625-2015-1-39-46

Cited by 5 publications

(2 citation statements)

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“…The determination of the number of activated basophils is a new, prospective test, both valid for the diagnosis of allergy and for evaluation of treatment effectiveness [12,13]. Thus, the data obtained in the study makes it possible to conclude that the involvement of magneto-laser therapy in the treatment of children with bronchial asthma is accompanied by positive dynamics of the immune status indicators.…”

Section: Discussionmentioning

confidence: 94%

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The Effect of Magneto-Laser Therapy on the Phenotype of Basophils in Children With Atopic Asthma

Asiryn¹,

Novikov²,

Matsiushchanka³

et al. 2019

Public Health

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In recent years there has been growing evidence to suggest a major role of basophils alongside eosinophils and mast cells in allergic inflammation. The aim of this study was to analyze the dynamics of the basophil phenotypes after the use of magneto-la-ser therapy in children with atopic bronchial asthma. Materials and methods. A total of 66 children with mild persistent atopic bronchial asthma (aged 6 to 18 years old) were examined. Group A included 34 children who received magneto-laser therapy together with basic asthma treatment (low dose of inhaled corticosteroids). Group B included 32 children who received only basic asthma therapy. The level of CD203с + , CD203с + CD63 + , CD203с + IgE + basophils was determined in peripheral blood in the beginning of the study, after 2 weeks and after 3 months. Results. A statistically significant decrease in the absolute levels of CD203с+CD63+ and CD203с + IgE + basophils and in the relative level of CD203с + IgE + among all CD203с + basophils was determined in group A after magneto-laser therapy. The comparison of group A and group B indices revealed a significant difference between the relative level of CD203с + IgE + basophils after 12-15 days from the beginning of the study. This indicator was significantly lower in group A than in group B (p<0.05). The absolute level of CD203с + IgE + basophils was significantly lower in group A in comparison with group B after 82-90 days (p<0.05). Conclusions. Magneto-laser therapy can change the phenotype of basophils in children with atopic bronchial asthma, causing suppression of proallergic pa-rameters. Considering these results there is reason to believe, that it is possible to use this method as an additional immunocorrective treatment in patients with basophilic phenotype of atopic asthma.

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Section: Discussionmentioning

confidence: 94%

“…A correlation between the level of basophils in sputum and eosinophils in both blood and the respiratory tract was found [9]. Degranulation of basophils is accompanied by the appearance of activation molecules -CD203c and CD63 [10,11,12].…”

Section: Discussionmentioning

confidence: 95%

The Effect of Magneto-Laser Therapy on the Phenotype of Basophils in Children With Atopic Asthma

Asiryn¹,

Novikov²,

Matsiushchanka³

et al. 2019

Public Health

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Basophil activation: theoretical aspects and use in the diagnosis of allergic diseases

Бычкова¹

2021

Med. immunol.

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Diagnostics of allergic diseases is a difficult issue, which requires distinct solutions, since this disorder is very common among the population. The overview focuses on complex diagnostics, including various methods that are most in demand at the present stage. The allergy diagnostics primarily include taking anamnesis, physical examination, instrumental and functional tests. Less often, the provocative tests are used, due to risk of severe adverse reactions. At the present stage, the role of laboratory diagnostics of allergies is growing, since, firstly, there is an increase in difficult-to-diagnose cases that require involvement of the entire medical armamentarium, and, secondly, the sensitivity and specificity of laboratory tests are improving. Among laboratory methods, the most significant are the assessment of the level of specific IgE, and the relatively new basophile activation test. The latter test is the main focus of the present review. It is functional and combines the advantages of provocative tests, during which conditions are created for the interaction of a potential allergen and effector cells of allergic inflammation, keeping safety for the patient. The data on the life cycle of basophils, their expression of membrane receptors, the content of granules, and ability to produce additional inflammatory mediators by the cells are presented. Participation of these cells in pathogenesis of allergic inflammation is being considered. Various mechanisms of basophil activation are discussed, both IgE-mediated and IgE-independent, which are similar in vivo and in vitro. Theoretical aspects of using the in vitro basophil activation test to estimate the hypersensitivity to a wide range of allergens are discussed. High sensitivity and specificity of the test for diagnosing allergies to food, household, pollen, insect and drug allergens are presented. Specific features of the basophil activation test related to the preanalytical, analytical and postanalytical stages of the study are highlighted. The factors influencing evaluation of this method are known. For example, difficulties in interpreting the test may arise while taking glucocorticosteroid hormones, in acute period of inflammation, with severe edema. The possibility of using this test to assess effectiveness of allergen-specific and anti-IgE therapy is being considered. A comparison of the basophil activation test, measurement of specific IgE and skin tests by various parameters related to performance and interpretation of results is carried out. Comprehensive diagnostics of allergic diseases, including usage of pathogenetically determined laboratory methods, will contribute to adequate treatment and, as a result, improve the health of the population.

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CD3<sup>+</sup>CD294<sup>+</sup>T cells of the type 2 immune response: their role in allergic inflammation

Бычкова

2022

Med. immunol.

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T lymphocytes type 2 immune response protect against helminths and toxins, and also contribute to the development of allergic inflammation. One of the most specific T cell surface markers T lymphocytes 2 is the CRTH2 molecule (CD294), which is an activating receptor for prostaglandin D2. The CD3+CD294+ population is negligible in the peripheral blood of healthy individuals; an increase occurs in patients with allergic diseases and an autoimmune nature of the response. The aim of the study was to study the quantitative and functional characteristics Т lymphocytes type 2 immune response in patients with rhinoconjunctival symptoms (n = 248) and drug hypersensitivity (n = 184). In 68 patients with an elevated and extremely high number of CD3+CD294+ cells, a detailed phenotype of this population was characterized by flow cytometry and the functional activity of the studied cell population in relation to the production of interleukin 4 and interferon γ was studied using enzyme immunoassay. The control group consisted of 34 volunteers. The relative number of CD3+CD294+ cells was significantly higher in the group of patients with drug hypersensitivity – 1.6% and rhinoconjunctival symptoms 1.2% compared to the control group – 1.0%. Elevated (1.6-3.6%) and extremely high (>3.6%) CD3+CD294+ cell number were significantly more frequently detected in patients with drug hypersensitivity. In both groups, an increase in the number of CD3+CD294+ cells were observed with severe damage to the skin. The phenotype of the population T lymphocytes type 2 CD45RA-CD3+CD294+CD2+CD5+CD7+CD27+CD28+CD57-CCR7- was determined, which corresponds to effector memory T lymphocytes. With a moderately increased relative amount of this population, T lymphocytes 2 were usually represented by T helpers 2. A pronounced increase in the population was observed due to T cytotoxic lymphocytes 2. Regardless of the predominance of the Т helper or Т cytotoxic 2 cells in patients revealed an increase in spontaneous production of interleukin 4 at a normal level of interferon. An increase in the peripheral blood T lymphocytes with CD294 expression contributes to the development, maintenance and exacerbation of allergic inflammation with the participation of IgEdependent and IgE-independent mechanisms. The CD3+CD294+ cell population should be determined as an additional parameter in assessing the presence of sensitization in the basophil activation test in patients with hypersensitivity reactions. The use of this laboratory biomarker to assess the dominant type of immune inflammation will make it possible to personalize the therapy of the examined patients. Identification of pronounced deviations of indicators from the average values of a population will influence the tactics of patient management.

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Features of Immune Response and Basophil Activation in Children With Chronic Urticaria

Cited by 5 publications

References 0 publications

The Effect of Magneto-Laser Therapy on the Phenotype of Basophils in Children With Atopic Asthma

The Effect of Magneto-Laser Therapy on the Phenotype of Basophils in Children With Atopic Asthma

Basophil activation: theoretical aspects and use in the diagnosis of allergic diseases

CD3<sup>+</sup>CD294<sup>+</sup>T cells of the type 2 immune response: their role in allergic inflammation

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