Fecal Carriage of ESBL-Producing E. coli and K. pneumoniae in Children in Guinea-Bissau: A Hospital-Based Cross-Sectional Study

Isendahl, Joakim; Turlej-Rogacka, Agata; Manjuba, Cristovão; Rodrigues, Amabélia; Giske, Christian G.; Nauclér, Pontus

doi:10.1371/journal.pone.0051981

Cited by 91 publications

(104 citation statements)

References 34 publications

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“…Our results disagree with many studies all over the world that reported bla CTX-M was the predominant gene among ESBL-producing E. coli isolated from fecal samples of healthy subjects [4,23,24], which may be explained by the variability in resistance patterns and encoding genes according to different geographic regions.…”

Section: Discussioncontrasting

confidence: 99%

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Bassyouni

Gaber

Wegdan

2015

J Infect Dev Ctries

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Introduction: Commensal E. coli can be considered a reservoir of genes coding for antibiotic resistance that may be transmitted in hospitals by healthcare workers (HCWs). This study aimed to determine the fecal carriage rate of extended-spectrum β-lactamase (ESBL)-producing E. coli among HCWs. Methodology: Stool samples were collected from 200 HCWs. Phenotypic screening for ESBL and AmpC β-lactamases was performed using disk diffusion and minimum inhibitory concentration methods followed by the combined disks test and double synergy differential test for confirmation. Multiplex polymerase chain reaction (PCR) was used to detect bla SHV , bla TEM , bla CTX-M , and CIT groups for AmpC genes. Results: Of 200 E. coli isolates, 100% were susceptible to imipenem, and 59 (29.5%) were resistant to one or more third-generation cephalosporins. By molecular analysis, 21% (42/200) were colonized by ESBL-producing E. coli, and 3% (6/200) were colonized by AmpCproducing E. coli. The bla SHV gene was the predominant ESBL gene, detected in 81.8% of the resistant E. coli isolates. Conclusions: These findings highlight the increase in fecal carriage of E. coli carrying ESBL and AmpC genes among HCWs, which may be one of the causes of the spread of ESBL-producing bacteria in hospitals and requires sound infection control measures. This is the first study of the fecal carriage rate of E. coli carrying AmpC genes in HCWs.

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Section: Discussioncontrasting

confidence: 99%

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Bassyouni

Gaber

Wegdan

2015

J Infect Dev Ctries

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“…Most of the isolates in the present study were resistant to multiple antibiotic groups. High resistance to common antibiotics has also been reported in previous studies [7,11,26]. The present study offers the first characterization of TEM-type and SHV-type ESBL variants produced by E. coli circulating in hospitals in Hamadan (northwest Iran).…”

Section: Discussionsupporting

confidence: 77%

“…Isendahl et al [11] reported that 32.6% fecal samples from children were carriers of ESBLproducing E. coli or K. pneumonia. Shahid et al [22] reported findings similar to those of the present study: 38.4% of the isolates in their study were positive for bla genes, and the bla TEM gene was more prevalent than the bla SHV gene.…”

Section: Discussionmentioning

confidence: 99%

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The Distribution of Beta Lactamase Genes in Escherichia Coli Phylotypes Isolated from Diarrhea and UTI Cases in Northwest Iran

Hemati¹,

Ghanbarpour²,

Alizade³

2014

Adv Clin Exp Med

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Background. Pathogenic Escherichia coli strains are a common cause of intestinal and extra-intestinal infections, especially in developing countries. Extended spectrum beta-lactamases (ESBL S ), a heterogeneous group of plasmidencoded beta-lactamases, are common throughout the world. Objectives. The aim of the present study was to determine the phenotypic and genotypic characteristics of ESBL S produced by E. coli isolates taken from patients with diarrhea and urinary tract infections (UTI) in northwest Iran. Material and Methods. A total of 132 E. coli isolates (92 isolates from UTI and 40 isolates from diarrheic cases) were recovered and confirmed by biochemical tests. The isolates were examined for bla TEM and bla SHV genes and phylogenetic background by two multiplex PCR assays. The isolates were tested for antibiotic susceptibility against nine antibiotic agents by the disk diffusion method. Results. The phylogenetic analysis showed that the UTI isolates mostly fell into phylo-group B2, followed by D, while the diarrheic isolates belonged to phylo-groups D and A. Out of 92 UTI isolates, 29.3% and 17.4% possessed bla TEM and bla SHV genes, respectively. Ten diarrheic isolates were positive for bla TEM, two isolates possessed the bla SHV gene, and one isolate was positive for both genes. The UTI isolates that were positive for bla TEM and bla SHV genes mostly belonged to phylo-groups D and B2, whereas the diarrhea isolates were in phylo-groups D and A. Phylogenetic group D isolates have an accumulation of ESBL S genes in the diarrheic and UTI isolates. In both the UTI and diarrhea isolates, the maximum rate of resistance was against cefazolin, and the minimum rate of resistance was against nitrofurantoin. Twenty-four antibiotic resistance patterns were observed among the isolates. The amikacin, ciprofloxacin, cefotaxime, cefuroxime, cefazolin, gentamicin, nalidixic acid and trimethoprim/sulfamethoxazole resistance pattern was the most prevalent in the isolates that belonged to phylo-group D. Conclusions. The correct choice of effective antibiotic policy is needed to limit the spread of antibiotic resistance in bacteria (Adv Clin Exp Med 2014, 23, 4, 523-529).

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“…Es importante mencionar que en los últimos años se ha descrito el incremento de enterobacterias productoras de BLEE de la familia CTX-M (19) , en este estudio se encontró que la gran mayoría de los aislados productores de BLEE presentaban en gen bla CTX-M, el grupo de gen dominante en los estudios de portadores fecales de República Checa (15) , España (20) , India (2) , Madagascar (16) , Nigeria (17) , Francia (24) , Guinea-Bissau (25) y Japón (26) poniendo de relieve la importancia de este gen en la difusión global de BLEE. La BLEE tipo CTX-M tiene principalmente actividad sobre la cefotaxima y no sobre la ceftazidima; aunque en los últimos años la emergencia de variantes de CTX-M (CTX-M-15, CTX-M-16, CTX-M-27 y CTX-M-19) están mejorando su actividad frente a ceftazidima.…”

Section: Discussionunclassified

Enterobacterias productoras de Betalactamasas de espectro extendido en muestras fecales en el Instituto Nacional de Salud del Niño, Perú

Aliaga

Sevilla-Andrade

Escalante

2015

Rev Peru Med Exp Salud Publica

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Objetivos. Describir la frecuencia de enterobacterias productoras de betalactamasas de espectro extendido (BLEE) en muestras fecales en el Instituto Nacional de Salud del Niño, Lima, Perú. Materiales y métodos. Se analizaron muestras de heces recibidas entre julio de 2012 y marzo de 2013, se trabajó con colonias sospechosas de ser enterobacterias productoras de BLEE que se desarrollaron en el agar Karmali; se realizó la identificación bioquímica por métodos convencionales y la confirmación del fenotipo BLEE. El análisis genotípico para detectar el gen de betalactamasa de la familia CTX-M se realizó por PCR. Resultados. De 235 muestras fecales se aisló un 64,2% de enterobacterias productoras de BLEE siendo Escherichia coli 86,1%, Klebsiella pneumoniae 7,9%, Salmonella sp. 2,6%, Enterobacter cloacae 2,0% y Proteus mirabilis 1,3%. El 89,1% de las enterobacterias productoras de BLEE presentaron el gen bla CTX-M. Se encontró una alta resistencia al ácido nalidíxico 84,8%, ciprofloxacina 74,2% y trimetoprimsulfametoxazol 81,5%. La resistencia a la amikacina fue de 1,3% y todos los aislados fueron sensibles al imipenem y meropenem. Conclusiones. Se encontró una alta frecuencia de enterobacterias productoras de BLEE en muestras fecales de pacientes ambulatorios atendidos en los consultorios externos y emergencia del Instituto Nacional de Salud del Niño en Perú. Palabras clave: beta-Lactamasa I; Heces; Enterobacterias (fuente: DeCS BIREME). EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL)-PRODUCING ENTEROBACTERIACEAE IN FECAL SAMPLES AT THE NATIONAL INSTITUTE OF CHILD HEALTH, PERU ABSTRACTObjectives. To describe the frequency of extended-spectrum beta-lactamase (ESBL)-producing enterobacteriaceae in fecal samples at the National Institute of Child Health, Lima, Peru. Materials and methods. Stool samples received between July 2012 and March 2013 with colonies suspected to be ESBL-producing enterobacteriaceae that developed in Karmali agar were analyzed. Conventional methods were performed for biochemical identification and the confirmation of the ESBL phenotype. Genotypic analysis to detect the beta-lactamase gene CTX-M family was performed by PCR. Results. Of the 235 fecal samples analyzed, 64.2% of ESBL-producing enterobacteria was isolated being 86.1% Escherichia coli, 7.9% Klebsiella pneumoniae, 2.6% Salmonella sp, 2.0% Enterobacter cloacae, and 1.3% Proteus mirabilis. 89.1% of the ESBL-producing enterobacteria presented the CTX-M gene. We found high resistance to nalidixic acid 84.8%, 74.2% ciprofloxacin and trimethoprim-sulfamethoxazole 81.5%.The resistance to amikacin was 1.3% and all isolates were susceptible to imipenem and meropenem. Conclusions. A high frequency of ESBL-producing enterobacteria was found in fecal samples of outpatients seen in the outpatient and emergency departments of the National Institute of Child Health of Peru.

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Fecal Carriage of ESBL-Producing E. coli and K. pneumoniae in Children in Guinea-Bissau: A Hospital-Based Cross-Sectional Study

Cited by 91 publications

References 34 publications

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

The Distribution of Beta Lactamase Genes in Escherichia Coli Phylotypes Isolated from Diarrhea and UTI Cases in Northwest Iran

Enterobacterias productoras de Betalactamasas de espectro extendido en muestras fecales en el Instituto Nacional de Salud del Niño, Perú

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