Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene

Abstract

This study aimed to reveal the microbial diversity in the fecal samples of bactrian camels using the 16S rRNA sequencing analysis on the Illumina MiSeq platform. Three fecal samples were collected from two geographical regions in China. Operational taxonomic unit (OTU) clustering was performed by identifying an OTU at 97% sequence identity. The alpha and beta diversities were applied to estimate the differences in microbial diversity among the three fecal samples. Totally, 4409, 3151 and 4075 OTUs in the fecal samples were identified in the Lop Nor wild camel (Camelus ferus), the domestic camel (C. bactrianus) and Dunhuang wild camel (C. ferus), respectively. The majority of bactreria were affiliated with phylum Firmicutes and Bacteroidetes in the three samples. The wild camels had higher gastrointestinal tract microbial diversity than the domestic one, while the microbial composition of the Lop Nor wild camel shared higher similarity with domestic camel at the genus and family levels than that of the Dunhuang wild camel did. Our results may provide a theoretical basis for assessing their health conditions and may thus be useful for protecting the critically endangered species of C. ferus.

Soil Microbial Community Structure and Diversity around the Aging Oil Sludge in Yellow River Delta as Determined by High-Throughput Sequencing

“…Because four kinds of reversible termination were combined with dNTPs, natural competition with minimum deviation detects the base sequence directly by virtue of each cycle of a fluorescence signal. Now, the high-throughput sequencing method is widely used in the process of microbial diversity analysis, since it has the characteristics of no culture, high sensitivity, and low detection limit [ 15 , 16 ].…”

Diversity of the fecal microbiota in Chinese ponies

“…Firmicutes can promote the decomposition of fibred into short-chain fatty acids ( 37 ). SCFAs (Short-chain fatty acids) can promote the absorption of calcium and induce the production of hormone-like insulin growth factor (IGF-1), which can promote bone development and affect bone health ( 38 ). A high F/B ratio and superior abundance of Firmicutes promote enhanced nutrient absorption in the GuZh horses, thus may be contributing to their big size.…”

Accelerated start-up, long-term performance and microbial community shifts within a novel upflow porous-plated anaerobic reactor treating nitrogen-rich wastewater via ANAMMOX process

“… 20 The proposed PCR amplification was conducted in Eppendorf Mastercycler under the condition described by Yuan and coworkers. 25 Furthermore, 1% agarose was also employed to amplify products, analyzed and finally sequenced qualified DNA using the Illumina Miseq sequencing platform. Primer sequences, short sequences, and low-quality sequences were removed after the sample reads had been filtered.…”