Fecal, oral, blood and skin virome of laboratory rabbits

Abstract

Here, we investigated the fecal, oral, blood, and skin virome of 10 laboratory rabbits using a viral metagenomic method. In the oral samples, we detected a novel polyomavirus (RabPyV), and phylogenetic analysis based on the large T antigen, VP1 and VP2 regions indicated that the novel strain might have undergone a recombination event. Recombination analysis based on related genomes confirmed that RabPyV is a multiple recombinant between rodent-like and avian-like polyomaviruses. In fecal samples, three partial or complete genome sequences of viruses belonging to the families Picobirnaviridae, Parvoviridae, Microviridae and Coronaviridae were characterized, and phylogenetic trees were constructed based on the predicted amino acid sequences of viral proteins. This study increases the amount of genetic information on viruses present in laboratory rabbits.

Metagenomic analysis of viromes in tissues of wild Qinghai vole from the eastern Tibetan Plateau

“…Two PBV contigs were obtained and PCR-confirmed from two fecal pools, with lengths of 1685/1684 bp. The distributions of these sequences were coincident with other PBVs, which have been detected in the feces of human, rabbit, dog, pig, rat, and bird 5,41 . Further analyses of these two segments revealed 2 RdRp region of PBV.…”

“…PBV is small, nonenveloped viruses with a segmented linear dsRNA genome, segments size ranges from 1.7 to 2.5 kb. In which, the large segment encodes a capsid preceursor, while the small one encodes the viral RNA-dependent RNA polymerase (RdRp) 5 . PBVs have been detected in the feces of many different hosts, including humans, rabbits, dogs, pigs, rats, and birds 5,41 .…”

The intestinal virome: lessons from animal models

“…A recent study also confirmed the distinction in virome composition between the large and the small intestine in rhesus monkey and pigs with a higher phage diversity in the lumen and predominance of eukaryotic viruses predominate in mucosal samples [40]. Recently, the faecal, oral, blood, and skin virome of laboratory rabbits were described leading to the identification of a novel polyomavirus [41].…”

Revolutionized virome research using systems microbiology approaches

“…144,145 Recently, rabbits have been used, leading to the identification of a novel polyomavirus. 146 Finally, swine models have also been used for virome in fecal virome transplants, which have been shown to act against necrotizing enterocolitis in pigs. 147…”