Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers

Bianco, Kayo; Barreto, Camila; Oliveira, Selma Soares de; Pinto, Leonardo Henriques; Albano, Rodolpho Mattos; Miranda, Catia Chaia de; Clementino, Maysa M.

doi:10.2166/wh.2015.292

Cited by 5 publications

(16 citation statements)

References 43 publications

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“…Quanto ao período de publicação dos artigos, 1 foi publicado em 2009 (Copeland et al, 2009), 2 (18,2%) em 2014 (Krolik et al, 2014;Nescerecka et al, 2014), 3 (27,3%) em 2015 (Bianco et al, 2015;Rodrigues et al, 2015;Sorensen et al, 2015), 3 (27,3%) em 2016 (Odagiri et al, 2016;Giglio et al;Weiss et al; e 2 estudos (18,2%) em 2018 (Diston et al, 2018;Malla et al, 2018).…”

Section: Resultsunclassified

“…Das publicações selecionadas, 3 (27,3%) tiveram como local de estudo o Brasil (Copeland et al, 2009;Bianco et al, 2015;Rodrigues et al, 2015), e quanto aos demais artigos, os estudos ocorreram no Canadá (Krolik et al, 2014), Letônia (Nescerecka et al, 2014), Zâmbia Research, Society and Development, v. 9, n. 9, e51996824, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i9.6824 8 (Sorensen et al, 2015), Índia (Odagiri et al, 2016), Itália (Giglio et al;, Nicarágua (Weiss et al;, Suíça (Diston et al, 2018) e Nepal (Malla et al, 2018 Quanto ao biomarcador utilizado, 5 (45,4 %) dos estudos selecionados utilizaram apenas bactérias (Krolik et al, 2014;Bianco et al, 2015;Sorensen et al, 2015;Giglio et al;Weiss et al;Malla et al, 2018), 2 (18,2%) bactérias e vírus (Rodrigues et al, 2015;Diston et al, 2018), 1 (9,1%) bactérias, vírus e protozoários (Odagiri et al, 2016), 1 estudo utilizou bactérias e arqueas (Bianco et al, 2015), 1 estudo utilizou bactérias, protozoários e Imunoglobulina humana (Copeland et al, 2009), e outro, bactérias e ATP (Nescerecka et al, 2014).…”

Section: Resultsunclassified

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Bioindicadores alternativos da qualidade da água para consumo humano

Daronco

Bárta

Silva

et al. 2020

RSD

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A segurança microbiológica da água está relacionada à contaminação fecal, e os biomarcadores convencionais de contaminação da água para consumo humano não permitem revelar qual é a fonte de contaminação fecal. O objetivo da pesquisa é encontrar evidências científicas sobre biomarcadores alternativos que possam identificar a fonte de contaminação fecal na água destinada ao consumo humano. Trata-se de uma pesquisa qualitativa, descritiva, tipo revisão integrativa de literatura. Biomarcadores utilizados foram bactérias, vírus, protozoários e arqueas, e 72,7% dos estudos tiveram amostras provenientes de águas subterrâneas. Taxas de detecção de marcadores genéticos Bacteriodales específicos de humanos foram maiores do que de animais na maioria dos estudos, destaca-se este achado em águas subterrâneas. Marcadores arqueais foram mais prevalentes que Bacteriodales nos corpos de água estudados. A variabilidade sazonal da ocorrência dos biomarcadores de contaminação fecal foi identificada em 60% dos estudos que realizaram essa avaliação. A sensibilidade e a especificidade dos marcadores espécie-específicos podem variar de acordo com a região estudada. Sugere-se que o uso desses marcadores deve ser combinado ao monitoramento de biomarcadores de contaminação fecal convencionais. Percebe-se a necessidade de mais pesquisas que utilizem biomarcadores que identifiquem possíveis fontes de contaminação humana e/ou animal na água de consumo humano, e que avaliem risco e incidência de doenças de veiculação hídrica nos locais estudados.

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Section: Resultsunclassified

Bioindicadores alternativos da qualidade da água para consumo humano

Daronco

Bárta

Silva

et al. 2020

RSD

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“…Sample collection was split between dry and wet weather in the Sao Jao river basin of Rio de Janeiro. Archaea markers were more prevalent in the feces and water bodies than bacteria (Bianco et al, 2015). However, the same is not true for other studies (Gourmelon et al, 2007).…”

Section: Tracking Methodsmentioning

confidence: 55%

“…Different types of microorganisms can be used for MST (Bianco et al, 2015;Jofre, Blanch, Lucena, & Muniesa, 2014). Bianco et al (2015) used both bacterial microorganisms as biological markers of certain species, as well as archaeal microbes.…”

Section: Tracking Methodsmentioning

confidence: 99%

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Nonpoint Source Bacterial Pollution in the Coyote Creek Watershed

Wilkinson

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NONPOINT SOURCE BACTERIAL POLLUTION IN THE COYOTE CREEK WATERSHED by Elisabeth Wilkinson Nonpoint source pollution is a global challenge affecting streams and rivers. Due to the highly dispersed nature of nonpoint sources, they are difficult to track and manage. The goal of this study was to assess the extent of bacterial pollution along Coyote Creek in Northern California and to provide insight on microwatershed characteristics and their potential correlation to bacteria loads. Samples were collected and analyzed for fecal indicator bacteria concentrations at 21 geographic locations from within the creek and its stormwater outfalls. E. coli levels within Coyote Creek and stormwater entering the creek exceeded recreational water quality standards. Stormwater outfall samples tended to have higher bacteria concentrations than creek samples. The average concentration for stormwater outfall samples was 439% higher than the average for creek samples. Increased bacterial concentrations were found in creek samples draining adjacent to industrial microwatersheds when compared to samples draining residential microwatersheds. Bacteria levels from creek samples showed weak positive correlations with the number of historical encampments upstream of each sample site. This study concludes that bacterial load mitigation efforts should focus on industrial land uses and prioritize stormwater retention and treatment. The effects of homeless encampments on bacterial load may be less of a concern than other sources. Recommendations include further investigation of sources contributing to elevated bacteria levels exiting stormwater outfalls using DNA tracking methods. v ACKNOWLEDGMENTS I could not have completed my thesis without the support received from family, friends, mentors, and San José State faculty and students. Thank you to my fellow San José State Students who volunteered their time to help with data collection in the field. Thank you to my committee members Rachel, Sabine, and Pat for your guidance, knowledge, and encouragement throughout the thesis process. Thank you to my mentors at the Santa Clara Valley Water District for technical expertise, equipment assistance, and for a rewarding internship experience that expanded my interest in stormwater management. Thank you to Bill, who not only offered countless hours of field assistance but also provided the upmost support, understanding, and patience. Lastly, thank you to my parents for your support throughout my entire education and for serving as a continuous example of dedication and hard work. vi TABLE OF CONTENTS List of Tables .

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Detection of human fecal contamination by nifH gene quantification of marine waters in the coastal beaches of Rio de Janeiro, Brazil

Oliveira

Sorgine

Bianco

et al. 2016

Environ Sci Pollut Res

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The identification of fecal pollution in aquatic ecosystems is one of the requirements to assess the possible risks to human health. In this report, physicochemical parameters, Escherichia coli enumeration and Methanobrevibacter smithii nifH gene quantification were conducted at 13 marine waters in the coastal beaches of Rio de Janeiro, Brazil. The pH, turbidity, dissolved oxygen, temperature, and conductivity, carried out by mobile equipment, revealed varied levels due to specific conditions of the beaches. The bioindicators' enumerations were done by defined substrate method, conventional, and real-time PCR. Six marine beach sites (46 %) presenting E. coli levels in compliance with Brazilian water quality guidelines (<2500 MPN/100 mL) showed nifH gene between 5.7 × 10 to 9.5 × 10 copies. L revealing poor correlation between the two approaches. To our knowledge, this is the first inquiry in qPCR using nifH gene as a biomarker of human-specific sources of sewage pollution in marine waters in Brazil. In addition, our data suggests that alternative indicator nifH gene could be used, in combination with other markers, for source tracking studies to measure the quality of marine ecosystems thereby contributing to improved microbial risk assessment.

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Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers

Cited by 5 publications

References 43 publications

Bioindicadores alternativos da qualidade da água para consumo humano

Bioindicadores alternativos da qualidade da água para consumo humano

Nonpoint Source Bacterial Pollution in the Coyote Creek Watershed

Detection of human fecal contamination by nifH gene quantification of marine waters in the coastal beaches of Rio de Janeiro, Brazil

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