Fed-batch culture under illumination with blue light emitting diodes (LEDs) for astaxanthin production by Haematococcus pluvialis

Lababpour, Abdolmajid; Shimahara, Kazumichi; Hada, Keishi; Kyoui, Yoshiaki; Katsuda, Tomohisa; Katoh, Shigeo

doi:10.1263/jbb.100.339

Cited by 44 publications

(25 citation statements)

References 4 publications

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“…To the best of our knowledge, the biomass density, volumetric astaxanthin yield, and productivity attained here in the perfusion culture with or without H 2 O 2 are the highest ever reported for various X. dendrorhous cultures in batch, fed-batch, or continuous modes. The maximum astaxanthin productivity achieved in this study, 4.86 mg/L-d, is comparable to, or even higher than those in the Haematococcus pluvialis algal cultures, for example, 4.62 mg/L-d in fed-batch culture under blue light illumination (Lababpour et al, 2005) and 3.22 mg/L-d in highcell-density mixotrophic culture (Zhang et al, 1999). Compared with the H. pluvialis algal cultures, the X. dendrorhous yeast culture usually has a higher growth rate and a higher maximum cell density but much lower astaxanthin content of cell ($ 1 mg/g cell vs. 30 mg/g cell or higher with H. pluvialis) (Todd Lorenz and Cysewski, 2000).…”

supporting

confidence: 63%

Perfusion culture process plus H₂O₂ stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Liu

2006

Biotech & Bioengineering

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A semicontinuous perfusion culture process (repeated medium renewal with cell retention) was evaluated together with batch and repeated fed-batch processes for astaxanthin production in shake-flask cultures of Xanthophyllomyces dendrorhous. The perfusion process with 25% medium renewal every 12 h for 10 days achieved a biomass density of 65.6 g/L, a volumetric astaxanthin yield of 52.5 mg/L, and an astaxanthin productivity of 4.38 mg/L-d, which were 8.4-fold, 5.6-fold, and 2.3-fold of those in the batch process, 7.8 g/L, 9.4 mg/L, and 1.88 mg/L-d, respectively. The incorporation of hydrogen peroxide (H(2)O(2)) stimulation of astaxanthin biosynthesis into the perfusion process further increased the astaxanthin yield to 58.3 mg/L and the productivity to 4.86 mg/L-d. The repeated fed-batch process with 8 g/L glucose and 4 g/L corn steep liquor fed every 12 h achieved 42.2 g/L biomass density, 36.5 mg/L astaxanthin yield, and 3.04 mg/L-d astaxanthin productivity. The lower biomass and astaxanthin productivity in the repeated fed-batch than in the perfusion process may be mostly attributed to the accumulation of inhibitory metabolites such as ethanol and acetic acid in the culture. The study shows that perfusion process plus H(2)O(2) stimulation is an effective strategy for enhanced astaxanthin production in X. dendrorhous cultures.

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confidence: 63%

Perfusion culture process plus H₂O₂ stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Liu

2006

Biotech & Bioengineering

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“…In addition, under illumination with the blue LEDs, the astaxanthin concentration increased to 16 μg/ml at light intensity of 8.0 μmol‐photon/m 2 s while at 12.0 μmol‐photon/m 2 s it increased to 25 μg/ml (astaxanthin content 6.5% of dry‐cell weight). Lababpour and colleagues () also reported the use of blue LEDs at 12.0 μmol‐photon/m 2 s in H. pluvialis mixtotrophic culture to increase astaxanthin content in this algae, producing 70 μg/cm 3 of astaxanthin in 400 h culture. Park and colleagues () studied flashing light as light source for high astaxanthin production by H. pluvialis .…”

Section: Natural Pigmentsmentioning

confidence: 96%

Extraction and purification of high‐value metabolites from microalgae: essential lipids, astaxanthin and phycobiliproteins

Cuéllar‐Bermúdez

Aguilar-Hernández

Cárdenas‐Chávez

et al. 2014

Microbial Biotechnology

410

210

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The marked trend and consumers growing interest in natural and healthy products have forced researches and industry to develop novel products with functional ingredients. Microalgae have been recognized as source of functional ingredients with positive health effects since these microorganisms produce polyunsaturated fatty acids, polysaccharides, natural pigments, essential minerals, vitamins, enzymes and bioactive peptides. For this reason, the manuscript reviews two of the main high-value metabolites which can be obtained from microalgae: pigments and essential lipids. Therefore, the extraction and purification methods for polyunsaturated fatty acids, astaxanthin, phycoerythrin and phycocyanin are described. Also, the effect that environmental growth conditions have in the production of these metabolites is described. This review summarizes the existing methods to extract and purify such metabolites in order to develop a feasible and sustainable algae industry.

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“…8. Lo anterior coincide con lo reportado por [25], [26], [27], quienes establecen que a longitudes de onda cortas (380-470nm) se puede inducir la acumulación de altas cantidades de astaxantina en H. pluvialis. Día En los resultados obtenidos, se ve la importancia de este tipo de prototipos ya que a la hora de realizar investigaciones en bioprocesos, estos permiten un monitoreo y control riguroso de las diferentes variables de proceso, garantizando la calidad en los bioprocesos y dando independencia al personal científico [6], además de permitir el aumento de la reproducibilidad de los procesos de producción rutinarios, logrando mayor eficacia para la investigación fisiológica causal-analítica, la verificación de los modelos de procesos, y el desarrollo de bioprocesos.…”

Section: B Pigmento Rojo (Astaxantina)unclassified

Monitoring and Control System used in Microalgae Crop

Beltrán

Castro

Garces

et al. 2012

IEEE Latin Am. Trans.

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This paper presents a monitoring and control system developed with the purpose to increase the reproducibility of microalgae production processes, achieving more effectiveness of casual-analytic physiologic research, the verification of process models and the development of bioprocess. It also controls of form web the following variables: temperature and photoperiod, the equipment was tested on a Haematococcus pluvialis crop. The settings of experimentation were: photoperiod 18/6 (light hours/dark) given by the extensions of blue SMD LEDs (380<λ<470 nm), the agitation speed of 720 RPM and temperature of 24°C. The settings for the controlled crop were the same except the light that, for this case, was natural. It was found that the system is able to control automatically the process variables permitting the continuous monitoring of the experiments, removing barriers of time and place. At an institutional level, the system has brought benefits allowing the decongestion of places as saving time for both students and researchers. At a level of bioprocesses of researching, it was determined that both kinetic of microalgae growth and quantity of astaxanthin that is produced are incremented when the crop is illuminating by short wavelength, blue light.

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Fed-batch culture under illumination with blue light emitting diodes (LEDs) for astaxanthin production by Haematococcus pluvialis

Cited by 44 publications

References 4 publications

Perfusion culture process plus H₂O₂ stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Perfusion culture process plus H₂O₂ stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Extraction and purification of high‐value metabolites from microalgae: essential lipids, astaxanthin and phycobiliproteins

Monitoring and Control System used in Microalgae Crop

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Fed-batch culture under illumination with blue light emitting diodes (LEDs) for astaxanthin production by Haematococcus pluvialis

Cited by 44 publications

References 4 publications

Perfusion culture process plus H2O2 stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Perfusion culture process plus H2O2 stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Extraction and purification of high‐value metabolites from microalgae: essential lipids, astaxanthin and phycobiliproteins

Monitoring and Control System used in Microalgae Crop

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Perfusion culture process plus H₂O₂ stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

Perfusion culture process plus H₂O₂ stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous