Fed-batch enzymatic hydrolysis of plantain pseudostem to fermentable sugars production and the impact of particle size at high solids loadings

Hernández‐Beltrán, Javier Ulises; Fontalvo, Javier; Hernández‐Escoto, Héctor

doi:10.1007/s13399-020-00669-2

Cited by 10 publications

(3 citation statements)

References 46 publications

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“…MFRs simulate bioreactor conditions under microliter scales, allowing for a large screening of different conditions and the ability to scale up the results [122]. Beltrán et al [123] used MFRs to test the impact of particle size during enzymatic hydrolysis. To optimize the conditions of the hydrolysis, they tested three levels of pH (4.5, 5, and 5.5) and temperature (45, 50, and 55 • C) and four levels of enzyme loading (1.0, 3.0, 5.0, and 7.0 µL/0.015 g-biomass), resulting in a total of 108 experiments.…”

Section: Different Setups and Optimal Conditions Of The Fermentation ...mentioning

confidence: 99%

Perspectives and Progress in Bioethanol Processing and Social Economic Impacts

Yaverino-Gutiérrez,

Wong,

Ibarra-Muñoz

et al. 2024

Sustainability

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The liquid biofuel bioethanol is widely produced worldwide via fermenting sugars extracted from a variety of raw materials, including lignocellulose biomass, one of the world’s most abundant renewable resources. Due to its recalcitrant character, lignocellulose is usually pretreated by mechanical, chemical, and biological methods to maximize sugar recovery. Pretreated lignocellulose biomass undergoes a fermentation process performed sequentially or simultaneously to saccharification. The different fermentation strategies (e.g., separate or simultaneous hydrolysis and fermentation or co-fermentation) and conditions (e.g., inoculum type load, agitation, temperature, and pH) affect ethanol yield. Genetic modification of the inoculum has been focused recently to improve ethanol tolerance and as well as to use different sugars to enhance the performance of the microorganisms involved in fermentation. Nonetheless, these improvements result in a substantial increase in costs and have certain environmental costs. This review offers an overview of advancements in bioethanol production, with a primary focus on lignocellulosic feedstock, while also considering other feedstocks. Furthermore, it provides insights into the economic, social, and environmental impacts associated with bioethanol production.

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Section: Different Setups and Optimal Conditions Of The Fermentation ...mentioning

confidence: 99%

Perspectives and Progress in Bioethanol Processing and Social Economic Impacts

Yaverino-Gutiérrez,

Wong,

Ibarra-Muñoz

et al. 2024

Sustainability

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show abstract

“…Production of these first-generation feedstocks in large scale compete with the availability of arable lands and may adversely impact the local biodiversity. To overcome these issues, a promising strategy is to produce ethanol from second-generation feedstocks such as lignocellulosic materials that are derived from non-food crops (e.g., Miscanthus sinensis, switchgrass, willow, energy cane, and wood residues, among others) and agro-industrial residues such as wheat straw, corn cobs, rice straw, rice husks, plantain pseudostem, and sugarcane bagasse (Chovau et al 2013;Hernández-Beltrán and Hernández-Escoto 2018;Correa et al 2019;Hernández-Beltrán et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…This hydrolysis takes place in a noncorrosive environment, resulting in low operation and maintenance costs. However, the economic feasibility of this process is limited by the high cost of enzymes (Zhu and Zhuang 2012;Hernández-Beltrán and Hernández-Escoto 2018;Hernández-Beltrán et al 2021). A feasible strategy to reduce costs in enzymatic hydrolysis is to perform it at high total insoluble solids (TS), which is reached when biomass concentration is higher than 15 wt% (dry basis) (Fockink et al 2016;Chen and Liu 2017).…”

Section: Introductionmentioning

confidence: 99%

Fed-batch enzymatic hydrolysis of steam-exploded sugarcane bagasse

Moura

Silva

Filho

et al. 2023

BioRes

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Statistical design and mathematical modeling were used to investigate the fed-batch enzymatic hydrolysis of steam-exploded sugarcane bagasse (195 °C, 7.5 min). First, a Box-Behnken experimental design was used to evaluate the effect of enzyme loading (8, 24, and 40 FPU g-1 glucans of Cellic CTec3®), stirring speed (100, 150, and 200 rpm), and substrate total solids (5, 12.5, and 20 wt%) on the release of glucose equivalents (GlcEq, mostly glucose) after hydrolysis for 48 h in batch mode. A simplified kinetic model was used to fit the experimental data, in which specific activities in Cellic CTec3 were not differentiated, enzyme adsorption was ignored, and end-product inhibition was only attributed to glucose accumulation. The adjusted kinetic model was used to predict the effects of substrate and enzyme intermittent feedings in fed-batch hydrolysis experiments. Compared with batch experiments at 20 wt%, the proposed fed-batch procedure was able to increase GlcEq productivity by nearly 68% using the same enzyme loading, producing substrate hydrolysates containing 91.8 g L-1 GlcEq.

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High-solids enzymatic saccharification of starch-rich raw herbal biomass residues for producing high titers of glucose

Zhu

et al. 2023

Environ Sci Pollut Res

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Fed-batch enzymatic hydrolysis of plantain pseudostem to fermentable sugars production and the impact of particle size at high solids loadings

Cited by 10 publications

References 46 publications

Perspectives and Progress in Bioethanol Processing and Social Economic Impacts

Perspectives and Progress in Bioethanol Processing and Social Economic Impacts

Fed-batch enzymatic hydrolysis of steam-exploded sugarcane bagasse

High-solids enzymatic saccharification of starch-rich raw herbal biomass residues for producing high titers of glucose

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