Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid

Tang, Ya‐Jie; Zhong, Jian‐Jiang

doi:10.1016/s0141-0229(02)00066-2

Cited by 188 publications

(126 citation statements)

References 20 publications

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“…Along with glucose, other carbon sources and other medium nutrients may depress the synthesis of antibiotics (17). A comparative study using various carbohydrates showed that lactose is the best source of carbon for the promotion of mycelial growth and for the production of ganoderic acid and polysaccharides in the case of Ganoderma lucidum (25). For Average data with different letters differ statistically according to the Tukey test at a 5% probability.…”

Section: Effect Of Agitation and Carbohydrate Concentration Over The mentioning

confidence: 99%

“…On the other hand, the growth kinetics and the fungal morphology in broth fermentation are highly dependent on the culture conditions, such as carbon sources, C/N relation, initial pH and temperature, agitation intensity and aeration rate (20). A large amount of work has reported the effects of environmental parameters on the biomass concentration and on the yield of bioactive compounds by mushroom, but those researches are limited to few kinds of mushrooms (4,7,10,12,13,20,25).…”

Section: Introductionmentioning

confidence: 99%

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Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Vieira¹,

Liebl²,

Tavares³

et al. 2008

Braz. J. Microbiol.

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Basidiomycete fungi of the Polyporus genus are a source of secondary metabolites which are of medicinal interest as antibacterial compounds. As these substances are produced in a small amount by the fungi, the study of the cultivation conditions in vitro that could possibly optimize their production seems of major importance. The effects of glucose and lactose, pH and agitation on biomass concentration and on the specific growth rate caused by the basidiomycete Polyporus tricholoma were investigated. The initial pH (4.5, 6.5 and 8.5) was autoregulated at pH 5.5, and the agitation increased the mycelial growth and the specific growth rate. The high concentration of carbon sources (4%) increased biomass production. The lactose concentration and the absence of agitation were determinant in the production of antibacterial metabolites. The characterization of the antibacterial substance by GC-MS indicated a major compound, isodrimenediol, produced by the fungus Polyporus tricholoma with activity against Staphylococcus aureus.

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Section: Effect Of Agitation and Carbohydrate Concentration Over The mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Vieira¹,

Liebl²,

Tavares³

et al. 2008

Braz. J. Microbiol.

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“…Besides this, the inoculum level can be significantly reduced, since in G. lucidum pellet-type submerged fermentation, it is usually required at 10% (v/v) or above (Tang and Zhong, 2002;Liu and Zhang, 2007). On the other hand, the morphology of mycelia should be considered seriously during the development of submerged culture as it significantly affects the formation of metabolites and product recovery (Du et al, 2003;Papagianni, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…To meet the great demand for G. lucidum, artificial cultivation on solid substrates such as lignocellulosic materials, especially sawdust, supplemented with wheat or rice bran has been developed over the past several decades (Berovič et al, 2003), and is now a major method for producing G. lucidum products. However, the solid-state bioprocess has several significant disadvantages, including the requirement of a long production cycle and the continuous fluctuation of product quality (Tang and Zhong, 2002).…”

Section: Introductionmentioning

confidence: 99%

Sonoporation of Ganoderma lucidum Mycelium for High Biomass and Exopolysaccharide Productivity in Submerged Culture

Liu¹,

Zhang²,

Kuang³

et al. 2016

IJAB

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Ganoderma lucidum, known as Lingzhi in China and Reishi in Japan, is a famous medicinal mushroom that is commonly produced by solid-state fermentation. Recently, submerged culture has been considered as a promising alternative for production of G. lucidum. Herein, to provide a theoretical base for preparation of a homogenous inoculum for G. lucidum submerged culture we investigated the effect of sonoporation on G. lucidum mycelium. Submerged culture was also evaluated using the sonoporated mycelium. Microscopic investigation indicated that the mycelial pellets (0.55 g, dry weight) contained in 60 mL solution were almost completely disappeared within 20 s by sonoporation (20 kHz and 125 w). The homogenate obtained after sonoporation had a 3.75×106 cfu/mL cell concentration. The sonoporation caused rapid leakage of intracellular protein that increased linearly with time. The metabolic activity of the cells decreased rapidly after sonoporation. Sonoporation also negatively affected laccase activity. When the homogenate was used at 4.76% (v/v) inoculum level, the cultures produced biomass and exopolysaccharide of 15.75 and 0.54 g/L, respectively; these values were 129.26 and 45.95% higher than that of the control culture using a pellet-type inoculum without sonoporation. Optimal inoculum size was 5.88-6.97% (v/v). We conclude that sonoporation is an effective method for homogenization of G. lucidum mycelium, and sonoporated mycelium can improve productivity significantly in G. lucidum submerged culture and requires lower levels of starting inoculum.

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“…For the determination of ITs, mycelia were collected as above, mixed with 50× (1 g/50 ml) of 95% ethanol and kept overnight, and then extracted twice by 400 W ultrasound at 75 °C for 1 h. The supernatant was merged and measured (Fang and Zhong, 2002b;Tang and Zhong, 2002;Xiao et al, 2006): 0.1 ml of the sample diluent was heated in a test tube to dry solvent at 60 °C and then 0.2 ml 0.05 g/ml of vanillinglacial acetic acid solution and 0.5 ml of perchloric acid were added and kept at 60 °C for 20 min; finally, 5 ml of glacial acetic acid was added after cooling for 15 min. The IT content was measured under 550 nm and IT production was expressed as follows: IT production (mg/100 ml)=IT content (mg/g)×mycelia biomass (g/100 ml).…”

Section: Determination Of Mycelia Biomass and Intracellular Triterpenmentioning

confidence: 99%

Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS

Cui

Yang

2015

J. Zhejiang Univ. Sci. B

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Abstract:As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H 2 O and CO 2 , as well as cleavage of the D-ring.

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Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid

Cited by 188 publications

References 20 publications

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Sonoporation of Ganoderma lucidum Mycelium for High Biomass and Exopolysaccharide Productivity in Submerged Culture

Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS

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