Feedback Regulation of Nitrate Influx in Barley Roots by Nitrate, Nitrite, and Ammonium

King, Bryan J.; Siddiqi, M. Yaeesh; Ruth, Thomas J.; Warner, Robert L.; Glass, Anthony D. M.

doi:10.1104/pp.102.4.1279

Cited by 153 publications

(96 citation statements)

References 26 publications

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“…It is well known that the amino acids added to the hydroponic medium can inhibit inorganic nitrogen uptake as a nitrate source by the roots (Stoelken et al, 2010). Nitrate uptake is regulated by several feedback inhibitors such as nitrate itself (King et al, 1993) and products of ammonium assimilation, in particular free soluble amino compounds (Collier et al, 2003). Our finding agrees with previous amino acid fertilization experiments of plants reported by Guidi et al (1998) and Santa-Cruz et al (2000).…”

Section: Discussionsupporting

confidence: 83%

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

García

Madrid

Gimeno

et al. 1970

Span. j. agric. res.

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The aim of this research was to determine the effects on growth variables and leaf mineral concentration of tomato plants watered with nutrient solutions containing amino acids. Two separate experiments were then carried out to achieve this goal. In the first experiment, plants were watered with seven different nutrient solutions consisting of half-strength Hoagland solution supplemented with single (Alanine, Serine, Phenylalanine, Tyrosine) or combined (Ala + Ser; Phe + Tyr) amino acids, each at 0.2 mM of concentration. The control nutrient solution did not have any amino acids added. Relative to the control, growth variables were not affected by the presence of amino acids. In general, the mixture of Ala + Ser increased the leaf Ca 2+ concentration, and the aliphatic amino acid treatments favoured an increase in leaf K + , Fe, Cu, and Mn concentrations. In addition, amino acids with hydroxyl groups in their structure, Ser and Tyr, increased Mg 2+ concentration. In the second experiment, the nutrient solutions were supplemented with 0.05 mM of the same amino acids (T1). Control plants were irrigated with amino acid-free nutrient solution (T0). The concentration of Ca 2+ , K + , Mg 2+ , Fe, Cu, and Mn in the leaf also increased due the amino acids treatment. Chlorophyll contents in the leaves and amino acids compositions in the xylem sap and leaf water relation were also determined. In conclusion, the data reported in the two experiments point out that the application of amino acids to the nutrient solution has a beneficial effect on the leaf mineral status and on the chlorophyll concentration of the leaves.Additional key words: amino acid fertilization; biofertilizer; hydroponic culture; Solanum lycopersicum L. ResumenEfectos de la aplicación de amino ácidos en la solución nutritiva sobre la composición mineral y el crecimiento de plántulas de tomate En este trabajo se estudiaron los efectos sobre el crecimiento y la concentración mineral de hojas de plantas de tomate cultivadas con soluciones nutritivas con distinta composición en aminoácidos. Para ello se plantearon dos experimentos. En el primero las plantas se regaron con siete soluciones nutritivas 1/2 Hoagland que diferían en la composición de los aminoácidos, todos ellos a una concentración total de 0,2 mM (Ala, Ser, Fen y Tir aplicados de forma individual, dos con la combinación Ala+Ser y Fen+Tir, un control sin aminoácidos). Las variables de crecimiento no mostraron diferencias entre los tratamientos; sin embargo, se observaron cambios en las concentraciones de nutrientes aunque los efectos dependieron de los aminoácidos utilizados. La mezcla de Ala + Ser aumentó la concentración de Ca 2+ , mientras que los tratamientos de aminoácidos alifáticos favorecieron un aumento de K + , Fe, Cu y Mn, y aquellos con grupos hidroxilo (Ser y Tyr) aumentaron el Mg 2+ . En el segundo experimento las plantas se regaron con disolución 1/2 Hoagland (T0) o con disolución 1/2 Hoagland más una mezcla de los 4 aminoácidos (0,05 mM) ensayados (T1). En este experiment...

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Section: Discussionsupporting

confidence: 83%

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

García

Madrid

Gimeno

et al. 1970

Span. j. agric. res.

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“…To uncouple gene responses to inorganic N from those elicited by downstream products of inorganic N-assimilation we performed treatments of Arabidopsis seedlings with combinations of inorganic N (nitrate and ammonium), organic forms of N (e.g., Glu and Gln), and MSX, an inhibitor of glutamine synthetase (8) [supporting information (SI) Fig. 5].…”

Section: Resultsmentioning

confidence: 99%

Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1

Gutiérrez

Stokes

Thum

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

339

289

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“…From incubation experiments with constant NO $ − and varying NH % + concentrations applied simultaneously, inhibition of NO $ − net uptake in roots by NH % + has been reported in some studies (Glass, Thompson & Bordeleau, 1985 ;Lee & Drew, 1989 ;King et al, 1993 ;Chaillou et al, 1994 ;Kreuzwieser et al, 1997 ;, but few or no effects were observed in others (Smith & Thompson, 1971 ;Schrader et al, 1972 ;Oaks, Stulen & Boesel, 1979 ;Flaig & Mohr, 1992). Direct effects of NH % + on processes of NO $ − uptake have been assumed by some authors (Lee & Drew, 1989 ;King et al, 1993), whereas others have postulated that products of N assimilation such as amino compounds are responsible for the downregulation of NO $ − net uptake in roots simultaneously supplied with NH % + (Lee et al, 1992 ;Imsande & Touraine, 1994). This down-regulation is thought to be achieved by an internal cyling pool of amino compounds in the tree that can be expanded in the presence of excess N in the environment.…”

Section: Uptake Of Nitrogen By the Rootsmentioning

confidence: 99%

Consequences of high loads of nitrogen for spruce (Picea abies) and beech (Fagus sylvatica) forests

et al. 1998

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High loads of nitrogen to spruce and beech forests can result in a complete inhibition of NO $ − uptake by the roots of the trees. This conclusion is based on (a) a comparison of a field site continuously exposed to high loads of N and a N-limited site, (b) the results of N fertilization of a N-limited field site, and (c) laboratory experiments under controlled environmental conditions. From fertilization experiments in the field it appears that NH % + uptake might become inhibited subsequent to an excessive uptake of NH % + . Apparently, the inhibition of NO $ − uptake by high loads of N to forests is a consequence of an accumulation of organic amino compounds in the roots originating from phloem transport from the shoot to the roots. These amino compounds seem to signal the N demand of the shoot to the roots. At present this function cannot be attributed to an individual organic amino compound in beech or spruce, but Gln is a likely candidate in both species among other compounds, e.g. Glu in spruce or Asp in beech trees. Direct inhibition of NO $ − uptake by NH % + can be excluded from the present studies. The mechanism(s) by which elevated levels of particular organic amino compounds interact with NO $ − uptake remains to be elucidated. This (these) mechanism(s) seem to affect NO $ − influx rather than NO $ − efflux. As a consequence of this (these) mechanism(s), spruce and beech trees can prevent, within a certain physiological window, N over-nutrition when the roots are exposed to excessive amounts of inorganic N. However, inhibition of NO $ − and NH % + uptake by the roots makes more N available for leaching into the ground water and, in addition, for soil microbial processes that result in the production and re-emission of volatile N compounds into the atmosphere.At the ' Ho$ glwald ' site, continuously exposed to high loads of N, 20 % of the N input from throughfall into the spruce and beech plots is re-emitted as NO and N # O. However, the NO to N # O ratio is highly dependent on the tree species, with a preference for NO in the spruce and a preference for N # O in the beech plot. Since at least part of the NO emitted from the soil will be converted inside the canopy in the presence of ozone to NO # that might then be absorbed by the leaves, the portion of the N in the throughfall that will be released from the forest by gaseous N emission is higher in the beech than in the spruce plot. Leaching of NO $ − into the ground water is high in the spruce, but minute in the beech plot. However, this positive effect of beech on ground water quality is achieved at the expense of an enhanced release of radiatively active N gases into the troposphere.Key words : Atmospheric pollution, forest ecosystem, nitrogen allocation, nitrogen fluxes, regulation, nitrogen oxides, ammonia. Both atmospheric and pedospheric sources of nitrogen are available to plants. The contribution of atmospheric N is considered to be low in remote * To whom correspondence should be addressed E-mail : here!sun2.ruf.uni-fre...

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Feedback Regulation of Nitrate Influx in Barley Roots by Nitrate, Nitrite, and Ammonium

Cited by 153 publications

References 26 publications

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1

Consequences of high loads of nitrogen for spruce (Picea abies) and beech (Fagus sylvatica) forests

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