Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)

Packiam, Soosaimanickam Maria; Cosson, E.; Baskar, Kathirvelu; Ignacimuthu, S.

doi:10.1590/s1516-8913201500141

Cited by 15 publications

(7 citation statements)

References 25 publications

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“… 19 and it was found that cadmium and lead caused large scale DNA damage in insect. Very few studies examined DNA damage in context of biocontrol 18 , 61 . Shetty et al .…”

Section: Discussionmentioning

confidence: 99%

Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura

Kaur

Chadha

Kaur

et al. 2018

Sci Rep

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In search for ecofriendly alternatives to chemical insecticides the present study was conducted to assess the insecticidal potential of an endophytic fungus Schizophyllum commune and its mechanism of toxicity by studying genotoxic and cytotoxic effects as well as repair potential using Spodoptera litura (Fabricius) as a model. Different endophytic fungi were isolated and tested for their insecticidal potential against S. litura. Among the tested endophytic fungi maximum mortality against S. litura was exhibited by S. commune isolated from Aloe vera. Extended development, reduced adult emergence was observed in larvae fed on diet supplemented with fungal extract. In addition to it the fungus also has propensity to increase oxidative stress which leads to significantly higher DNA damage. The significantly lower frequency of living haemocytes and increased frequency of apoptotic and necrotic cells was also observed in larvae treated with fungal extract. The extent of recovery of damage caused by fungus was found to be very low indicating long term effect of treatment. Phytochemical analysis revealed the presence of various phenolics, terpenoids and protein in fungal extract. Biosafety analysis indicated the non toxic nature of extract. This is the first report showing the insecticidal potential of S. commune and the genotoxic and cytotoxic effects associated with it.

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“… 19 and it was found that cadmium and lead caused large scale DNA damage in insect. Very few studies examined DNA damage in context of biocontrol 18 , 61 . Shetty et al .…”

Section: Discussionmentioning

confidence: 99%

Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura

Kaur

Chadha

Kaur

et al. 2018

Sci Rep

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“…Raja et al (2005) identified two potential antifeedant compounds, namely 5-keto-pent-3, 4-enyl-2′phenol and 5-penty-methylene oxy-4, 4-dimethyl-cyclohexenol from H. suaveolens. Plant derived product Ponnem affected the DNA of H. armigera which was confirmed by comet assay ( Packiam et al, 2015 ). Two new oil formulations, namely neem oil, karanj oil and caryophyllene isolated from ethyl acetate extract of Hyptis suaveolens and neem oil, karanj oil and β-sitosterol isolated from ethyl acetate extract of M. Corchorifolia showed pest control properties at the laboratory condition ( Pavunraj et al, 2016 ).…”

Section: Introductionmentioning

confidence: 82%

Karyomorphological effects of two new oil formulations on Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Pavunraj

Baskar²,

Arokiyaraj

et al. 2021

Saudi Journal of Biological Sciences

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The cotton bollworm Helicoverpa armigera is a serious pest of many economically important crops. Since this pest has become resistant to the conventional synthetic insecticides, newer compounds and formulations are being developed against this insect pest. Many natural compounds isolated from the plants were tested against this pest. Among them Hyptis suaveolens and Melochia corchorifolia showed insecticidal properties against H. arnigera . Based on bioefficacy studies, caryophyllene and β-sitosterol were isolated from H. suaveolens and M. corchorifolia respectively. The isolated natural compounds were further developed as formulations in various combinations with neem ( Azadirachta indica ) and karanj ( Pongamia pinnata ) oils. The present study indicated that the formulations influenced the karyomorphology of H. armigera .

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“…To determine the toxicity of the essential oils, the leaf-dipping technique was followed [14] . To determine the lethal concentration(LC 50 ) and LC 90 , five concentrations were prepared in distilled water using 0.5% Triton™ X-100 (Sigma-Aldrich Corp., St. Louis, MO, USA), as a surfactant.…”

Section: Toxicity Assay Of the Plant Essential Oilsmentioning

confidence: 99%

“…DNA damage also was estimated in Culex quinquefasciatus using essential oil of Psoralea corylifolia with larvicidal and adulticidal effect [13] . Moreover, DNA damage and mutation were detected in H. armigera and Rhyzopertha dominica treated with various plant oils [14] .…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of Some Plant Essential Oils in Cotton Leafworm, Spodoptera Littoralis (Lepidoptera: Noctuidae): The Potential Role of Detoxification Enzymes

Fergani

El-Banna

Hamama

2020

Egyptian Journal of Zoology

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Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)

Abstract: Newly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20

Cited by 15 publications

References 25 publications

Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura

Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura

Karyomorphological effects of two new oil formulations on Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Genotoxicity of Some Plant Essential Oils in Cotton Leafworm, Spodoptera Littoralis (Lepidoptera: Noctuidae): The Potential Role of Detoxification Enzymes

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