Abstract:The objective of this experiment was to determine if lactational performance and energy partitioning by dairy cows would differ in response to dietary corn grain (CG) types [steam-flaked corn (SFC) vs. high-moisture corn (HMC)] and slow-release urea (SRU) supplementation. Eight multiparous Holstein cows (32 ± 8.2 d-in-milk) were used in a duplicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement to test 4 dietary treatments: SFC without SRU, SFC with SRU, HMC without SRU, and HMC with SRU. Supplementation of SRU tended to increase intakes of DM or increased crude protein intake under SFC, but no effect under HMC, leading to CG × SRU interactions on DM and crude protein intakes. Neither type of CG nor SRU supplementation affected milk production. The HMC fed at 14.3% DM allowed cows to partition more net energy into BW compared with those fed SFC diets, whereas supplementing SRU tended to decrease the portion of net energy partitioned into BW gain under both SFC and HMC diets. These collective results demonstrate that feeding HMC with SRU can be a practical option in high-forage lactation diets to maintain or improve nutrient and energy utilization efficiency.Key words: high-moisture corn, slow-release urea, lactating dairy cow, energy partition, ruminal fermentation.Abbreviations: ADF, acid detergent fiber; BW, body weight; CP, crude protein; DM, dry matter; DMI, dry matter intake; ECM, energy-corrected milk; FCM, 3.5% fat corrected milk; HMC, high-moisture corn; NDF, neutral detergent fiber; NE l , net energy for lactation; NFC, nonfiber carbohydrate; RDP, rumen degradable protein; RUP, rumen undegradable protein; SFC, steam-flaked corn; SRU, slow-release urea; TMR, total mixed ration; VFA, volatile fatty acids. Forages are the major component of diets for lactating dairy cows that provides energy and nutrients, and forage fiber is important for healthy cows, stimulating rumination and saliva production that aids in ruminal digestion and fermentation. Despite their multiple benefits to the cow, forages are not always efficiently utilized. For example, productive performance may be limited by excessive RDP from alfalfa, reduced availability of degradable starch, or a combination of both (Dhiman and Satter 1997). In addition, excessive physically effective NDF can reduce DMI of dairy cows, as a concentration of the physically effective NDF increases, the DMI decreases due to rumen fill (Zebeli et al. 2008). To increase energy availability to the cow, and therefore optimize nutrient utilization, highly digestible carbohydrates are commonly included when feeding high-forage diets. High-moisture corn (HMC) has consistently greater starch digestion in the rumen (Knowlton et al. 1998), the small intestine (Knowlton et al. 1998), and the total tract (Wilkerson et al. 1997; Knowlton et al. 1998; Firkins et al. 2001) compared with dry (Wilkerson et al. 1997; Knowlton et al. 1998; Firkins et al. 2001) and/or steam-flaked corn (SFC; Firkins et al. 2001). Eun et al. (2014) found that feeding HMC i...