Feeding with Single Strains Versus Mixed Cultures of Lactic Acid Bacteria and Bacillus subtilis KKU213 Affects the Bacterial Community and Growth Performance of Broiler Chickens

Buahom, Juthamas; Siripornadulsil, Surasak; Siripornadulsil, Wilailak

doi:10.1007/s13369-017-3045-6

Cited by 10 publications

(23 citation statements)

References 37 publications

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“…The comparison of the effects of B. subtilis KKU213 and/or P. pentosaceus NP6 revealed similar impacts in broilers and resulted in similar viable cell counts. Both KKU213 and NP6 were highly resistant to an acidic pH value of 2 [ 12 , 13 ], and thus, these strains can likely tolerate the low pH in the stomach of broilers, can multiply in this environment and facilitate the growth of indigenous Bacillus and LAB in the crop and jejunum (Figures- 2 and 3 ), which is consistent with previously observed results [ 10 , 12 ]. In general, similar microbial profiles were found among all probiotic-fed groups and the control.…”

Section: Discussionsupporting

confidence: 88%

“…We isolated Bacillus and LAB bacteria from several sources based on the described properties. Among the selected isolates, the bacteriocin-producing strain B. subtilis KKU213 has exhibited interesting potential due to its effects on promoting the growth of broilers [ 10 , 12 ]. P. pentosaceus NP6 inhibits several Salmonella serovars in vitro and in food matrices [ 13 ], but KKU213 does not exhibit this property.…”

Section: Discussionmentioning

confidence: 99%

“…B. subtilis KKU213 (KF220378), which was isolated from soil, is a bacteriocin producer and potential probiotic in broilers [ 12 ]. This strain was cultivated in Bacillus broth (1% peptone, 0.5% inulin, 0.5% NaCl, 0.5% beef extract, and 0.3% K 2 HPO 4 ) at 50°C with shaking at 150 rpm for 24 h. The three LAB strains used in this study include (i) Pediococcus pentosaceus NP6 (KT00456.1), which was isolated from fermented fish and is an effective Salmonella inhibitor in fish samples [ 13 ]; (ii) Pediococcus acidilactici SH8 (MF061302.1), which was isolated from fermented shrimp, can inhibit Gram-positive and Gram-negative bacterial pathogens and does not exert a negative impact on broilers [ 10 ]; and (iii) CH403, which was isolated from the intestine of a domestic Thai fowl and can inhibit a wide range of Gram-negative bacteria. All LAB strains were cultivated in de Man, Rogosa and Sharpe (MRS) broth under microaerobic conditions at 42°C for 48 h. All 18 serovars of S .…”

Section: Methodsmentioning

confidence: 99%

“…The inhibition of Salmonella infection by lactic acid bacteria (LAB) in intestinal epithelial cells has been documented [ 8 , 9 ]. Among bacterial species, several members of LAB and Bacillus , either as single or combined cultures, have been widely used as probiotics or microbial feed additives for chickens [ 10 , 11 ]. We have previously reported the potential of Bacillus subtilis KKU213 and some LAB strains as potential probiotics in broilers [ 10 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

et al. 2020

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Aim: The aim of the study was to determine the potentials and effects of Bacillus subtilis and lactic acid bacteria (LAB) as probiotics on broiler growth, health, and Salmonella infection. Materials and Methods: To evaluate the inoculum size applicable for broilers, 1-day-old broilers were orally fed fresh cultures of single strains and a B. subtilis KKU213/Pediococcus pentosaceus NP6 mixture at 108 and 1012 colony-forming unit (CFUs)/mL/chick. The body weight gain (BWG), Salmonella contamination level and total Bacillus and LAB abundances in the crop and intestine were measured. Subsequently, 1-day-old broilers were orally fed of KKU213, CH403, and Pediococcus acidilactici SH8 at 1010 CFUs/mL, followed by inulin. After 35 days, the BWG, Bacillus and LAB abundances in the cecum, blood parameters, and KKU213 colonization were assessed. Results: The broilers fed single strains or KKU213+NP6 exhibited a higher BWG and a higher crop LAB abundance than the controls (p<0.05). Probiotic feeding decreased the intestinal Salmonella abundance and correspondingly increased the LAB abundance. The broilers fed the mixed culture (KKU213+CH403+SH8) followed by prebiotics showed lower mortality, higher blood high-density lipoprotein levels, and lower blood uric acid levels than the controls (p<0.0004). Probiotic feeding significantly increased the Bacillus and LAB counts (p<0.05). A CE330 isolate obtained from the cecum after 35 days of KKU213 feeding was closely related to B. subtilis KKU213. Conclusion: B. subtilis KKU213 is a potent probiotic strain that can survive, colonize and reduce Salmonella infection in broilers and improve their growth and health. This strain, combined with different LAB can act synergistically in the gut and promote broiler growth.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

et al. 2020

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“…In previous studies, various species of probiotic bacteria such as L. reuteri [19,20], L. salivarius [19,21,16,22], Enterococcus faecium and Enterococcus durans [22]were isolated from chicken digestive tract. In the present study, four LAB isolates namely, IS3, IS4, IS6 and IS7 were identified based on sequence analysis of 16S rRNA gene.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Ethiopian Rhode Island Red Chicken and their Potential Use as Probiotics

Tsega¹,

Maina²,

Berhane³

2019

Preprint

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Background Chicken productivity and survival is affected by environmental stress and incidences of different diseases. After the ban of antibiotics growth promoters by different countries, the use of probiotics feed additives is mentioned as one alternative to antibiotics. The objective of this study was to isolate and identify potential probiotic lactic acid bacteria strains from the digestive tract content of Rhode Island Red chicken. Results The study was conducted in Gondar town of Amhara region, North West Ethiopia from November, 2018 to March, 2019. From the gastro intestinal content of 5 chicken 190 isolates were randomly selected. The isolates were identified and screened by their biochemical, morphological and 16S rRNA gene sequence. The in vitro probiotic potential of isolates was characterized by various tests. From 190 isolates 73 were found as gram positive, catalase negative and were able to grow at pH4. The bacteria were either rod (83.6%) or round (16.4%) shaped. Ten isolates were then randomly selected for further in vitro probiotic characterization due to higher cell surface hydrophobicity, good survival at pH2 and survival at 0.1% bile salt. From the 10 isolates 6 were able to survive at pH1 for 3hrs incubation. Five isolates (IS1, IS2, IS5, IS7 and IS8) were resistance to ampicillin, chloramphenicol, ciprofloxacin and erythromycin antibiotics. All the ten isolates showed antagonistic activity against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Staphylococcus intermedius and Salmonella enteritidis. The optimum temperature for all ten isolates was 450C and all the isolates were able to grow at 0.69 mol/L of NaCl, and were able to ferment galactose, glucose, fructose, mannitol, sorbitol and sucrose. Using phylogenetic analysis of 16SrRNA gene sequence; IS3 was identified as Lactobacillus salivarius while IS4, IS6 and IS7 were identified as Lactobacillus reuteri. Conclusion The study concludes that the 10 selected isolates can be able to survive the stress conditions of gastrointestinal tract and can thus be considered as potential probiotics candidates for chickens.

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Association of Probiotic Supplementation with Improvements in the Gut Microbes, Blood Lipid Profile and Caecal Villus Morphology of Broilers

et al. 2021

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The effects of probiotic supplementation vary among species or strains, and the dose and feeding timeline could alter the intestinal environment of broilers. A fresh culture of Bacillus subtilis CE330 and multiple lactic acid bacteria (LAB) strains was administered to hatched broilers to investigate their synergistic and long-term effects. One-day-old Cobb broilers (n 30) were randomly distributed into five groups (six chicks per group) and orally fed dH 2 O (control), B. subtilis CE330, or mixed cultures of B. subtilis CE330 and LAB at different doses and times. The simultaneous oral feeding B. subtilis CE330 and 4 LAB strains (ratio equal to 1:4) resulted in the best improvements in growth and health performance in broilers raised without antibiotics. The probiotic-fed group showed a 32.3% higher body weight gain and a 30.2% increase in high-density lipids. One of the 22 Bacillus-like isolates showed similar properties to B. subtilis CE330, including inhibition of bacterial pathogens, presence of the subtilosin gene, digestive enzyme activity, and antibiotic susceptibility. PCR-DGGE analysis revealed that the control group exhibited a diverse bacterial population of pathogen-related species that was not detected in the caecum of the B. subtilis CE330-fed group. The villus number and the morphology of the epithelial cells in the caecum were obviously increased and improved the nutrient adsorption in the probiotic-fed groups. The administration of three doses of a probiotic mixture early after hatching was sufficient for a long-term health benefit on broilers. The most effective probiotics could be further developed for the production of antibiotic-free broilers. Keywords Bacillus subtilis • Lactic acid bacteria • Probiotics • Broilers • Caecal villi • PCR-DGGE Abbreviations CA4 Enterococcus faecium CA4 CE330 Bacillus subtilis CE330 CH24 Lactobacillus salivarius CH24 CH33 Enterococcus durans CH33 SH8 Pediococcus acidilactici SH8 BS Bacillus subtilis CE330 B Wilailak Siripornadulsil

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Feeding with Single Strains Versus Mixed Cultures of Lactic Acid Bacteria and Bacillus subtilis KKU213 Affects the Bacterial Community and Growth Performance of Broiler Chickens

Cited by 10 publications

References 37 publications

Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

Characterization of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Ethiopian Rhode Island Red Chicken and their Potential Use as Probiotics

Association of Probiotic Supplementation with Improvements in the Gut Microbes, Blood Lipid Profile and Caecal Villus Morphology of Broilers

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