Feline mammary turnours*

Hayden, D. W.; Nielsen, S. W.

doi:10.1111/j.1748-5827.1971.tb06197.x

Cited by 55 publications

(29 citation statements)

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“…The age of affected cats, gross appearance of the tumors, as well as prevalence of benign versus malignant mammary tumors in this study were similar to those in previous reports [4,7,38-44]. The present study supports the previously reported higher prevalence of adenocarcinoma in abdominal mammarylobe [33,44,45].…”

Section: Discussionsupporting

confidence: 92%

RETRACTED ARTICLE: Comparative value of clinical, cytological, and histopathological features in feline mammary gland tumors; an experimental model for the study of human breast cancer

et al. 2013

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BackgroundThe diagnosis of breast lesions is usually confirmed by fine-needle aspiration cytology (FNAC) or histological biopsy. Although there is increasing literature regarding the advantages and limitations of both modalities, there is no literature regarding the accuracy of these modalities for diagnosing breast lesions in high-risk patients, who usually have lesions detected by screening. Moreover, few studies have been published regarding the cytopathology of mammary tumors in cats despite widespread use of the animal model for breast cancer formation and inhibition. The objective of the present study was to evaluate the diagnostic interest of cytological and histopathological analysis in feline mammary tumours (FMTs), in order to evaluate its possible value as an animal model.MethodsThe study was performed in 3 female cats submitted to surgical resections of mammary tumours. The mammary tumours were excised by simple mastectomy or regional mastectomy, with or without the superficial inguinal lymph nodes. Female cats were of different breeds (1 siamese and 2 persians). Before surgical excision of the tumour, FNA cytology was performed using a 0.4 mm diameter needle attached to a 8 ml syringe held in a standard metal syringe holder. The cytological sample was smeared onto a glass slide and either air-dried for May-Grünwald-stain and masses were surgically removed, the tumours were grossly examined and tissue samples were fixed in 10%-buffered-formalin and embedded in paraffin. Sections 4 μm thick were obtained from each sample and H&E stained.ResultsCytologically, atypical epithelial cells coupled to giant nucleus, chromatin anomalies, mitotic figures, spindle shape cells, anisocytosis with anisokaryosis and hyperchromasia were found. Histologically, these tumors are characterized by pleomorphic and polygonal cell population together with mitotic figures, necrotic foci and various numbers inflammatory foci. Also, spindle shaped cells, haemorrhage localized in the different regions, local invasiveness and enlarged nuclei were observed. The samples included 3 tumors of mammary glands mammary tumors were complex carcinomas (n = 2) and adenocarcinoma (n = 1). The histological grades of the 3 cases were as follows: grade II, (1/3); grade III, (2/3) with high mitotic index. The preferential localization of mammary neoplasms was in the inguinal lobe (1/3 case) and abdominal lobes (2/3 cases). Furthermore, 1case of the inguinal mass affected the left caudo-inguinal lobe and 2cases right cranio and caudo abdominal lobes.ConclusionThe study concluded that cytology could be used as a quick, rapid, field diagnostic technique in combination with histopathology for the diagnosis of feline mammary tumors (FMTs). Our findings in feline MTs indicate that FMTs could be useful as an animal model of human breast cancer. Moreover, because of the similarity of the cytohistopathological findings in the human and feline mammary gland tumours, it is possible to use the same cytopathological criteria applied in human pathology for th...

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Section: Discussionsupporting

confidence: 92%

RETRACTED ARTICLE: Comparative value of clinical, cytological, and histopathological features in feline mammary gland tumors; an experimental model for the study of human breast cancer

et al. 2013

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“…T he lymphatic system is the most common route of metastasis of mammary tumors in humans and animals and surgery of mammary neoplasms is considered to be surgery of their lymphatics 1–4 . Surgery of mammary tumors in animals usually comprises the removal of the tumor(s), the mammary gland(s), the lymphatic drainage, and the sentinel lymph node.…”

Section: Introductionmentioning

confidence: 99%

Computed Tomography and Radiographic Indirect Lymphography for Visualization of Mammary Lymphatic Vessels and the Sentinel Lymph Node in Normal Cats

Patsikas

Papadopoulou

Charitanti

et al. 2010

Veterinary Radiology &Amp; Ultrasound

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The potential of computed tomography indirect lymphography (CT-indirect lymphography) and radiographic indirect lymphography to demonstrate the draining lymphatic vessels and sentinel lymph node of normal mammary glands was tested in 31 healthy female cats. The lymphatic drainage of each mammary gland was studied initially by CT-indirect lymphography after intramammary injection of 0.5 ml of iopamidol, followed by images acquired at 1, 5, 15, and 30 min after injection. One day after CT-indirect lymphography, the lymph drainage of the mammary gland was assessed using radiographic in direct lymphography af terintramammary injection of 0.5 ml of ethiodized oil followed by radiographs made at 1, 5, 15, 30, 45, and 60 min after injection. The time between intramammary injection and opacification of the draining mammary lymphatic vessels and the sentinel lymph node, the duration of adequate opacification of the draining mammary lymphatic vessels and of the sentinel lymph node and also the number and course of draining mammary lymphatic vessels and location of sentinel lymph node were compared for CT-indirect lymphography vs. radiographic indirect lymphography in each examined gland. This results suggest that radiographic indirect lymphography is easy to perform and can be used for accurate demonstration of the draining lymphatic pathways of mammary glands in radiographs made at 5-30 min after injection. However, CT-indirect lymphography was able to better demonstrate small lymphatic vessels and accurately define the exact topography of the sentinel lymph node in images acquired at 1 min after injection.

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“…67(12): 1273-1276, 2005 Feline mammary cancer (FMC) is the third most common neoplasm following hematopoietic and skin tumors [1,6]. Most cases of FMC are histologically adenocarcinoma [3]. This tumor shows local infiltrative-destructive growth and frequently metastasizes to the regional lymph nodes, lung and pleura in the early stages of disease [6,15].…”

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confidence: 99%

Establishment and Characterization of Eight Feline Mammary Adenocarcinoma Cell Lines

Uyama

Hong

Nakagawa

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Eight new feline mammary adenocarcinoma cell lines derived from either primary or metastatic lesions were established. The morphology of all the cell lines was epithelioid and round to spindle in shape, with cell growth occurring in a monolayer fashion. On immunohistochemistry, these cells reacted with anti-keratin and anti-vimentin antisera. The doubling time of these cells was between 19 and 54 hr. Tumor masses were developed in nude mice by subcutaneous inoculation of the cells that were histologically identical to their original mammary tumor lesions. Telomerase activities measured using the telomeric repeat amplification protocol assay revealed high telemetric activity in all of the cells. KEY WORDS: feline, mammary tumor cell line, primary and metastatic lesion.J. Vet. Med. Sci. 67(12): 1273-1276, 2005 Feline mammary cancer (FMC) is the third most common neoplasm following hematopoietic and skin tumors [1,6]. Most cases of FMC are histologically adenocarcinoma [3]. This tumor shows local infiltrative-destructive growth and frequently metastasizes to the regional lymph nodes, lung and pleura in the early stages of disease [6,15]. Establishment of cell lines from FMC has been conducted, however the number of established cells has been limited [2,8,10], suggesting that the establishment of FMC cell lines is difficult compared to those of other animal species. FMC has many features, including specific biological behaviors and histopathological appearance as well as poor prognosis, and may be an attractive model for research on human breast cancer [7,9,15]. We report herein the establishment and characterization of eight FMC cells derived from either primary or metastatic lesions of five cats with spontaneous FMC.Tissue samples were obtained from 13 cats with FMC admitted to the Veterinary Hospital at the University of Tokyo (Fig. 1). Tumor cells were collected from the surgical specimen or by thoracocentesis in the cats with thoracic metastasis. Tissue samples were placed in 50-ml tubes with phosphate buffer solution (PBS) supplemented with 0.2-mg/ ml gentamycin sulfate (Sigma Chemical Co., St. Louis, MO, U.S.A.) and kept overnight at 4°C. They were then minced into 1-to 2-mm 3 pieces and digested with collagenase mixed solution of DNase and pronase (Sigma Chemical Co.) for 1 hr at 37°C under constant stirring. The digested cells were then incubated in RPMI 1640 (Nissui Pharmaceutical Co., Tokyo, Japan) supplemented with 20% fetal bovine serum (FBS) (Equitech-Bio Inc., Ingram, TX, U.S.A.), 0.01 mg/ml L-glutamine (Nissui Pharmaceutical Co.), fungizone (Gibco BRL., Grand Island, N.Y., U.S.A.) and 5 mg/l gentamycin sulfate (Sigma Chemical Co.), and incubated at 37°C in a humidified atmosphere of 5% CO 2 . Cells collected from pleural effusion were centrifuged and washed with PBS, then cultured under the same conditions described above. After the 10th passages, when cell growth seemed to be stable, the concentrations of FBS in the culture medium were decreased from 20% to 10%.Eight feline mammary adenoca...

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Feline mammary turnours*

Cited by 55 publications

References 1 publication

RETRACTED ARTICLE: Comparative value of clinical, cytological, and histopathological features in feline mammary gland tumors; an experimental model for the study of human breast cancer

RETRACTED ARTICLE: Comparative value of clinical, cytological, and histopathological features in feline mammary gland tumors; an experimental model for the study of human breast cancer

Computed Tomography and Radiographic Indirect Lymphography for Visualization of Mammary Lymphatic Vessels and the Sentinel Lymph Node in Normal Cats

Establishment and Characterization of Eight Feline Mammary Adenocarcinoma Cell Lines

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