Feline Panleukopenia virus infection in a captive-bred Bengal tiger (&lt;em&gt;Panther tigris tigris&lt;/em&gt;) and a leopard (&lt;em&gt;Panthera pradus&lt;/em&gt;)

Dissanayake, D. R. A.; Silva, I. D.; Gamage, Sashikala; Sonnadara, Dinuka; Bandara, M. R. B. N.; Alokabandara, S. S.; Jayapani, V. P. Priyanka; Jayaweera, W. R.

doi:10.4038/slvj.v63i2.12

Cited by 5 publications

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“…Tigers are the largest cat species and an important member of the ecosystem. Previous studies have identified the FPV infection in several populations of tigers [6,7]. This evidence indicates that tigers appear to be susceptible to FPV infection.…”

Section: Discussionmentioning

confidence: 70%

“…Duarte et al (2009) [ 6 ] reported on the death of a white tiger ( Panthera tigris ) and an African lion ( Panthera leo ) from FPV infection at the Lisbon zoo in Portugal. Dissanayake et al (2017) [ 7 ] reported on the death of an unvaccinated Bengal tiger cub ( Panthera tigris tigris ) and severe illness in an unvaccinated leopard cub ( Panthera pardus ) at a zoological garden in Sri Lanka, both of which were caused by FPV. These incidences indicated that tigers, which are the largest known cat species, appear to be particularly susceptible to FPV infection.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

et al. 2020

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Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccinations against FPV among wild felid species have long been practiced in zoos worldwide. However, few studies have assessed the tiger immune response post-vaccination due to the absence of a serological diagnostic tool. To address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody levels against the feline panleukopenia vaccine by employing the synthesized subunit capsid protein VP2. An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced in this study and employed in the assay. It was then compared to a commercial HRP-conjugated goat anti-cat IgG (HRPanti-cat IgG). Sensitivity and specificity were evaluated using the Bayesian model with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) tests. Results: The posterior estimates for sensitivity and specificity of two indirect ELISA HRP-conjugated antibodies were higher than those of the HI test. The sensitivity and specificity of the indirect ELISA for HRP-anti-tiger IgG and HRP-anticat IgG were 86.5, 57.2 and 86.7%, 64.6%, respectively, while the results of the HI test were 79.1 and 54.1%. In applications, 89.6% (198/221) and 89.1% (197/221) of the tiger serum samples were determined to be seropositive by indirect ELISA testing against HRP-anti-tiger and HRP-anti-cat, respectively.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

et al. 2020

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“…Kecerdasan buatan yang dimaksud disini merujuk pada mesin yang mampu berfikir, menimbang tindakan yang akan diambil, dan mampu mengambil keputusan seperti yang dilakukan oleh manusia [2]. [3].…”

Section: Pendahuluanunclassified

Sistem Pakar Menggunakan Metode Certainty Factor Dalam Akurasi Identifikasi Penyakit Panleukopenia Pada Kucing

Putra¹,

Nurcahyo²

2020

JSisfotek

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Feline Panleukopenia is a disorder caused by inflammation of Feline Parvovirus. This viral inflammation can trigger inflammation of the digestive system in cats and can also attack the cat's bone marrow, and the total white blood cells in the cat's body will decrease. At this time, many people do not know how to accurately identify the disease Feline Panleukopenia in cats and as a result of that cats who suffer from it experience illness and even death. This study aims to determine the level of accuracy in identifying Panleukopenia in cats. There are several types of symptoms that refer to Feline Panleukopenia. Furthermore, the data is processed manually with the Certainty Factor method and continued by using a website-based expert system software. The processing stages are rule solving, determining the weight value of each symptom and calculating the Certainty Factor value with the rule formula. The results of the data processing are continued with the calculation of the level of accuracy. The result of testing this method is that there are 100% of the 5 test data. Based on the accuracy of the identification results of this system, this study is very precise in the level of identifying the level of accuracy of feline panleukopenia. Expert testing systems have been able to specifically identify Feline Panleukopenia. Through the Certainty Factor method, the level of accuracy that can be obtained is quite accurate and can help veterinarians improve their accuracy to identify Feline Panleukopenia in cats.

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“…Presently, tigers in zoological parks are susceptible to several infectious diseases, including canine distemper (CDV) (Appel et al 1994;Nagao et al 2012), feline panleukopenia virus (FPV) (Duarte et al 2009;Dissanayake et al 2017), feline calicivirus (FCV) (Tian et al 2016), Cytauxzoon felis (Jakob and Wesemeier 1996;Lewis et al 2012), Toxoplasma gondii (Yang et al 2017) and even avian influenza (Keawcharoen et al 2004;Hu et al 2016). One explanation for the recent emergence of infectious diseases is that global changes coupled with human activities have potentially altered the interactions between the disease components (Daszak et al 2000;de La Rocque et al 2008;Harvell et al 2009;Gallana et al 2013;Lindahl and Grace 2015).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of polyclonal antibody against Bengal tiger (Panthera tigris tigris) immunoglobulin G

Areewong

Sangchantip

Rungphattanachaikul

et al. 2019

Journal of Applied Animal Research

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The immunoglobulin G (IgG) antibody is important for detecting and evaluating immune responses. Despite increasing opportunities for tigers to be exposed to emerging diseases due to global changes coupled with increasing interaction with humans, there is no available antibody specific to tigers. Aims of this study were to produce a rabbit anti-Bengal tiger (Panthera tigris tigris) IgG polyclonal antibody and to determine its specificity. The molecular weight of the purified Bengal tiger IgG was approximately 170 kDa. The cross-reactivity of rabbit anti-Bengal tiger IgG to IgG of other wild felid species and other domestic animals was analyzed by indirect enzyme-linked immunosorbent assay. The cross reactivity to other related wild animals ranged from 53.69% to 75.63%. There was high crossreactivity with domestic cats (63.58%) and pigs (38.45%) and low cross-reactivity with domestic cows (10.46%), goats (8.60%), sheep (7.25%), and chickens (6.91%). To confirm cross-reactivity with domestic animals' IgG, western blotting was performed. These results indicated that the purified rabbit anti-Bengal tiger IgG polyclonal antibody strongly reacted with species in the family Felidae. The polyclonal antibody generated in this study has the potential to aid in the development of useful tools for further novel immunological investigations on tigers and related felid species.

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Feline Panleukopenia virus infection in a captive-bred Bengal tiger (<em>Panther tigris tigris</em>) and a leopard (<em>Panthera pradus</em>)

Cited by 5 publications

References 0 publications

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

Sistem Pakar Menggunakan Metode Certainty Factor Dalam Akurasi Identifikasi Penyakit Panleukopenia Pada Kucing

Production and characterization of polyclonal antibody against Bengal tiger (Panthera tigris tigris) immunoglobulin G

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