Female C57BL/6 and BALB/c mice differently use the acoustic features of male          ultrasonic vocalizations for social preferences

Nomoto, Kensaku; Hashiguchi, Akiko; Asaba, Akari; Osakada, Takuya; Kato, Masahiro; Koshida, Nobuyoshi; Mogi, Kazutaka; Kikusui, Takefumi

doi:10.1538/expanim.19-0119

Cited by 9 publications

(9 citation statements)

References 20 publications

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“…In four male mice groups, dominant calls from the chaser stimulated the speed of locomotion in the recipient males, indicating that the USV can convey emotional information from the emitter to modulate social behavior in the recipient (Sangiamo et al, 2020). We reported playback experiments in which male USVs were regenerated by the ultrasound emitter and observed the behavioral changes in females (Asaba et al, 2014;Nomoto et al, 2018Nomoto et al, , 2020. These reports revealed that females can recognize some specific characteristics of male USVs and show approaching behavior in response to the USVs.…”

Section: Discussionmentioning

confidence: 93%

Testosterone Increases the Emission of Ultrasonic Vocalizations With Different Acoustic Characteristics in Mice

et al. 2021

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Testosterone masculinizes male sexual behavior through an organizational and activational effects. We previously reported that the emission of ultrasonic vocalizations (USVs) in male mice was dependent on the organizational effects of testosterone; females treated with testosterone in the perinatal and peripubertal periods, but not in adults, had increased USV emissions compared to males. Recently, it was revealed that male USVs have various acoustic characteristics and these variations were related to behavioral interactions with other mice. In this regard, the detailed acoustic characteristic changes induced by testosterone have not been fully elucidated. Here, we revealed that testosterone administered to female and male mice modulated the acoustic characteristics of USVs. There was no clear difference in acoustic characteristics between males and females. Call frequencies were higher in testosterone propionate (TP)-treated males and females compared to control males and females. When the calls were classified into nine types, there was also no distinctive difference between males and females, but TP increased the number of calls with a high frequency, and decreased the number of calls with a low frequency and short duration. The transition analysis by call type revealed that even though there was no statistically significant difference, TP-treated males and females had a similar pattern of transition to control males and females, respectively. Collectively, these results suggest that testosterone treatment can enhance the emission of USVs both in male and female, but the acoustic characteristics of TP-treated females were not the same as those of intact males.

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Section: Discussionmentioning

confidence: 93%

Testosterone Increases the Emission of Ultrasonic Vocalizations With Different Acoustic Characteristics in Mice

et al. 2021

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“…Such strain‐dependent patterns of call categories could affect mate‐choice and/or probability to interact with a conspecific. Additional studies are needed to better understand the role and characteristics of vocal communication during social interactions (e.g., differences between courtship and mating or USV production) across strains, since few studies have detailed this aspect so far 71,72 …”

Section: Discussionmentioning

confidence: 99%

“…Additional studies are needed to better understand the role and characteristics of vocal communication during social interactions (e.g., differences between courtship and mating or USV production) across strains, since few studies have detailed this aspect so far. 71,72 It is worth highlighting that USVs are a useful tool for evaluating emotion and motivation in rodents. 73,74 In juvenile and adult rats, call categories have been extensively used as measures of emotional/ affective state.…”

Section: Discussionmentioning

confidence: 99%

Ultrasonic vocalizations in laboratory mice: strain, age, and sex differences

Caruso

Marconi

Scattoni

et al. 2022

Genes Brain and Behavior

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Mice produce ultrasonic vocalizations (USVs) in different social contexts across lifespan. There is ethological evidence that pup USVs elicit maternal retrieval and adult USVs facilitate social interaction with a conspecific. Analysis of mouse vocal and social repertoire across strains, sex and contexts remains not well explored. To address these issues, in inbred (C57BL/6, FVB) and outbred (CD‐1) mouse strains, we recorded and evaluated USVs as neonates and during adult social encounters (male–female and female–female social interaction). We showed significant strain differences in the quantitative (call rate and duration of USVs) and qualitative vocal analysis (spectrographic characterization) from early stage to adulthood, in line with specific patterns of social behaviors. Inbred C57BL/6 mice produced a lower number of calls with less internal changes and shorter duration; inbred FVB mice displayed more social behaviors and produced more syllables with repeated internal changes; outbred CD‐1 mice had an intermediate profile. Our results suggest specific vocal signatures in each mouse strain, thus helping to better define socio‐communicative profiles of mouse strains and to guide the choice of an appropriate strain according to the experimental settings.

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“…Besides approach behavior, female mice exhibit a preference toward a particular type of male USVs. Studies showed that female mice stayed longer near a sound source of one type of USVs than that of another type of USVs in experiments in which two USVs were played back simultaneously ( Musolf et al, 2010 ; Asaba et al, 2014 ; Nomoto et al, 2020 ). Specifically, our previous studies demonstrated that female mice preferred male USVs of a different strain than themselves ( Asaba et al, 2014 ; Nomoto et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Studies showed that female mice stayed longer near a sound source of one type of USVs than that of another type of USVs in experiments in which two USVs were played back simultaneously ( Musolf et al, 2010 ; Asaba et al, 2014 ; Nomoto et al, 2020 ). Specifically, our previous studies demonstrated that female mice preferred male USVs of a different strain than themselves ( Asaba et al, 2014 ; Nomoto et al, 2020 ). These studies also revealed that pre-exposure of a male pheromone, exocrine gland-secreting peptide 1 (ESP1) ( Haga et al, 2010 ), was necessary for females’ USVs preferences.…”

Section: Introductionmentioning

confidence: 99%

Prelimbic cortex responds to male ultrasonic vocalizations in the presence of a male pheromone in female mice

Asaba

Nomoto²,

Osakada³

et al. 2022

Front. Neural Circuits

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Sensory signals are critical to perform adaptive social behavior. During copulation, male mice emit ultrasonic vocalizations (USVs). Our previous studies have shown that female mice exhibit approach behavior toward sound sources of male USVs and that, after being exposed to a male pheromone, exocrine gland-secreting peptide 1 (ESP1), female mice exhibited a preference toward a particular type of male USVs. These findings suggest that male USVs modulate female courtship behavior. However, it remains unclear which brain regions and what cell types of neurons are involved in neuronal processing of male USVs. To clarify this issue, immediate early gene analysis, behavioral analysis, and neurochemical analysis were performed. The in situ hybridization analysis of c-fos mRNA in multiple brain regions showed that neurons in the prelimbic cortex were responsive to presentation of male USVs in the presence of ESP1. Furthermore, this study found that activity of prelimbic cortex was correlated with the duration of female exploration behavior toward a sound source of the USVs. Finally, by using double immunohistochemistry, the present study showed that the prelimbic neurons responding to the presentation of male USVs were presumably excitatory glutamatergic neurons. These results suggest that the prelimbic cortex may facilitate female courtship behavior in response to male USVs.

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Female C57BL/6 and BALB/c mice differently use the acoustic features of male ultrasonic vocalizations for social preferences

Cited by 9 publications

References 20 publications

Testosterone Increases the Emission of Ultrasonic Vocalizations With Different Acoustic Characteristics in Mice

Testosterone Increases the Emission of Ultrasonic Vocalizations With Different Acoustic Characteristics in Mice

Ultrasonic vocalizations in laboratory mice: strain, age, and sex differences

Prelimbic cortex responds to male ultrasonic vocalizations in the presence of a male pheromone in female mice

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