Fabrication
of 3D cell scaffolds has gained tremendous attention
in recent years because of its applications in tissue engineering
and cell biology applications. The success of tissue engineering or
cell interactions mainly depends on the fabrication of well-defined
microstructures, which ought to be biocompatible for cell proliferation.
Femtosecond-laser-based 3D printing is one of the solution candidates
that can be used to manufacture 3D tissue scaffolds through computer-aided
design (CAD) which can be efficiently engineered to mimic the microenvironment
of tissues. UV-based lithography has also been used for constructing
the cellular scaffolds but the toxicity of UV light to the cells has
prevented its application to the direct patterning of the cells in
the scaffold. Although the mask-based lithography has provided a high
resolution, it has only enabled 2D patterning not arbitrary 3D printing
with design flexibility. Femtosecond-laser-based 3D printing is trending
in the area of tissue engineering and cell biology applications due
to the formation of well-defined micro- and submicrometer structures
via visible and near-infrared (NIR) femtosecond laser pulses, followed
by the fabrication of cell scaffold microstructures with a high precision.
Laser direct writing and multiphoton polymerization are being used
for fabricating the cell scaffolds, The implication of spatial light
modulators in the interference lithography to generate the digital
hologram will be the future prospective of mask-based lithography.
Polyethylene glycol diacrylate (PEG-DA), ormocomp, pentaerythritol
tetraacrylate (PETTA) have been fabricated through TPP to generate
the cell scaffolds, whereas SU-8 was used to fabricate the microrobots
for targeted drug delivery. Well-designed and precisely fabricated
3D cell scaffolds manufactured by femtosecond-laser-based 3D printing
can be potentially used for studying cell migration, matrix invasion
and nuclear stiffness to determine stage of cancer and will open broader
horizons in the future in tissue engineering and biology applications.