Femtosecond optical trapping of cells: Efficiency and viability

Gong, Jixian; Li, Fang; Xing, Qirong

doi:10.1007/s12209-009-0055-9

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…When cells are active and growing well, methylene blue cannot enter the cell to stain them. 37 However, when cell activity is impaired, the permeability of the cell membrane changes. This leaves the cell unable to resist the entry of external substances, 34,38 so methylene blue can enter the cell and dye the cell blue.…”

Section: Bio-reduction Of Indigomentioning

confidence: 99%

pH-Controlled bio-reduction of indigo with S. cerevisiae whole-cell biotransformation

Cheng

Gong

et al. 2022

Textile Research Journal

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Indigo is one of the most widely used dyes in history, but its application is currently greatly limited due to the lack of clean and efficient reduction methods. Aimed at the long-term consumption of traditional bio-reduction of indigo and the ecological problems existing in the application of chemical reducing agents, a high-efficiency indigo dyeing method based on pH-controlled bio-reduction of Saccharomyces cerevisiae under an aerobic environment at normal temperature was constructed. The results showed that the reduction ability of the bio-reduction system and its effect on dye reduction was closely related to the growth and metabolism of cells and the pH of the system. It is worth noting that a suitable alkaline environment is a key to improving the reduction capacity and shortening the reduction time. Under the dyeing pH condition of 10, the K/S of the fabric could reach 6.2 and exhibited the greatest color strength with good fastness after dyeing for 4 days. A pH-controlled bio-reduction strategy with whole-cell biotransformation was designed to construct an indigo green dyeing system with high efficiency and less environmental pollution.

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Section: Bio-reduction Of Indigomentioning

confidence: 99%

pH-Controlled bio-reduction of indigo with S. cerevisiae whole-cell biotransformation

Cheng

Gong

et al. 2022

Textile Research Journal

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“…When speckle pattern is retroflected by a phase conjugating mirror, the 'rope' structured intertwining vortices can be formed [16], which can further broaden the applications of the optical vortices. Some groups have also focused on the optical tweezers application using the femtosecond laser as the light source [17][18][19]. Compared to continuous wave tweezers, the prominent advantage of femtosecond tweezer is that the high peak power of femtosecond pulses is able to induce nonlinear optical process within the trapped particle such as multi-photon absorption, second harmonic generation and so on.…”

Section: Introductionmentioning

confidence: 99%

Manipulating ellipsoidal micro-particles by femtosecond vortex tweezers

Liu

Guo

et al. 2015

J. Opt.

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We study the manipulation of the ellipsoidal micro-particles by the femtosecond vortex tweezer experimentally and theoretically. In our setup the micro-particles can be rotated stably by means of optical torque induced by the femtosecond optical whirl. In order to give insight into observed effects, we establish two adequate theoretical models: one is based upon assumption of full absorption and the other uses the ray tracing method. The numerical simulations agree well with the experimental results.

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Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species

Krishna,

Burrow,

Jiang

et al. 2024

J. Biomed. Opt.

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Optical tweezers (OTs) have emerged as an essential technique for manipulating nanoscopic particulates and biological specimens with sub-micron precision and have revolutionized various fields, including biology and colloidal physics. However, traditional optical trapping techniques often rely on moderate-to highpower continuous wave (CW) lasers, which can introduce unwanted thermal effects and photodamage to delicate samples. An innovative alternative has emerged through the utilization of femtosecond (fs) lasers at ultra-low average powers on the order of tens of microwatt. Unexpectedly overlooked until now, this method enables the direct trapping and manipulation of cells without relying on functionalized spheres.Aim: We aim to compare the trap stiffness of CW and fs lasers in an unexplored average power regime (sub-1 mW) on cells within the intermediate-size regime.Approach: A CW or fs laser is used to trap cells in an inverted microscope setup. We trap five different pathogenic bacteria with different morphologies to compare trap stiffness.Results: We find that fs laser-assisted selective holding with ultra-low power (FLASH-UP) exhibits five times greater trap stiffness than CW-based OTs and can trap at lower intensities. Furthermore, we demonstrate that FLASH-UP does not impact cell motility.Conclusions: FLASH-UP displays higher trap stiffness at average powers below 1 mW and does not impact cell functionality. These results pave the way for ultralow-power trapping of cells for applications in sorting, bio-sensing, in vivo cell manipulation, and single-cell analysis.

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Femtosecond optical trapping of cells: Efficiency and viability

Cited by 3 publications

References 25 publications

pH-Controlled bio-reduction of indigo with S. cerevisiae whole-cell biotransformation

pH-Controlled bio-reduction of indigo with S. cerevisiae whole-cell biotransformation

Manipulating ellipsoidal micro-particles by femtosecond vortex tweezers

Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species

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