Fermentation of methanol in the sheep rumen

Pol, Alma C van de; Demeyer, Daniël

doi:10.1128/aem.54.3.832-834.1988

Cited by 50 publications

(23 citation statements)

References 13 publications

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“…In the present study CG had 46.5% of lipids and 8.7% of methanol. However, the high-risk to health associated to methanol consumption due to inclusion of CG in diets of ruminant animals is not expected since methanol is naturally produced in the ruminal environment as a result of pectin digestion (Pol & Demeyer, 1988). These authors have demonstrated that a continuous infusion of methanol (1 mol L −1 ) at a rate of 10 mL h −1 into the rumen of ovine was completely converted to methane.…”

Section: Resultsmentioning

confidence: 99%

Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat

et al. 2014

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Thirty non-castrated male lambs with 20±2.3 kg average body weight (BW) were randomly assigned to five treatments consisted of different dietary concentrations of crude glycerin (CG; 0, 3, 6, 9 and 12% on DM basis) to evaluate the effects on performance, carcass and meat quality traits. A quadratic effect was observed for performance (P=0.04), final BW (P<0.01) and hot carcass weight (P<0.01). No effects of CG were observed (P>0.05) on carcass pH neither on shear-force, cooking loss and ether extract content in longissimus. The inclusion of CG tended to reduce the Zn content in meat (P=0.09). The data suggests that CG (36.2% of glycerol and 46.5% of crude fat) may be used in diets of finishing lambs with concentrations up to 3% without negative effects on performance and main carcass traits. Moreover, inclusion of CG seems to not affect quality and safety of meat for human consumption.

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Section: Resultsmentioning

confidence: 99%

Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat

et al. 2014

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“…Findings from the current study established that the geographical region in conjunction with the host species has a higher in uence on the abundance of Methanomassiliicoccales than the hydrogenotrophic archaea. The difference in the abundance of Methanomassiliicoccales between cattle and buffaloes could be due to the variable production of methylated substrate compounds in the rumen, which may be mediated through diet [64]. In addition, the differences in the environmental conditions in uence the archaeal community structure [28].…”

Section: Discussionmentioning

confidence: 99%

Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Trivedi¹,

Malik²,

Kolte³

et al. 2020

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Background Archaea belong to the domain Euryarchaeota, represents only 3-5% of the rumen microbiota; however, they play a central role in maintaining the H2 pressure. Host species in conjunction with geographical regions may lead to the variable distribution of rumen methanogens. Indian cattle and buffaloes represent 13 and 53% of the global population, respectively. However, the influence of host species (bovine) and geographical regions on the methanogens distribution has not been investigated in Indian subcontinent. Therefore, the present study was undertaken to explore the rumen methanogens diversity in the cattle and buffaloes native to the two distinct geographical regions of India. Results Next generation sequencing of 16S rRNA gene from 36 rumen fluid samples corresponding to cattle and buffaloes revealed that Methanobrevibacter (Mbr.) genus constituted largest fraction (55-62%) of the rumen archaeal community. Furthermore, Methanobrevibacter gottschalkii was the most prominent methanogens with the significantly higher abundance in cattle. Methanomassiliicoccales represented <10% of the total archaeal community and was found to be the most variable between these two host species. In north region, the distribution of Methanomassiliicoccales, such as Methanosphaera sp., Group 4, Group 8, Group 12 sp. and Group 9 sp. was significantly higher in cattle. The distribution of prominent methanogens, such as Mbr. gottschalkii and Mbr. ruminantium did not differ between two host species in the north region. However, Mbr. gottschalkii was significantly higher in buffaloes as compared to cattle in south region. Mbr. wolinii was exclusively detected in the cattle. Inter-region comparison established that Methanomassiliicoccales were the most variable between the two regions. Conclusions Host species did not affect the distribution of hydrogenotrophic methanogens except for Mbr. gottschalkii. Methylotrophic methanogens, in spite of their limited representation, were most influenced by the host species and geographical regions. Overall, the Methanomassiliicoccales distribution was higher in cattle, and the environmental conditions in north region were conducive for their higher distribution. The variable distribution of methanogens indicated that host and geographical region oriented strategies needs to be developed for the reduction of enteric methane emission. Further studies are warranted to explore the impact of diet on the distribution of rumen methanogens between the host species within and across different environmental conditions.

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“…For example, the K m for formate was lower for fumarate reducers compared to methanogens (Asanuma et al, 1999). In the rumen, methanol and methylamines resulting from the metabolism of pectin (Pol and Demeyer, 1988) and betaine and choline (Neill et al, 1978;Mitchell et al, 1979) can be used by methylotrophic methanogens as substrates for CH 4 production. Importantly, reductive acetogens have also been reported to use methanol and methylamines as [H] donors (Ragsdale and Pierce, 2008;Jeyanathan et al, 2014), including the rumen acetogen Eubacterium limosum, a methanol-utilizer (Genthner et al, 1981).…”

Section: The Competition For Dihydrogenmentioning

confidence: 99%

Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions

2020

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Rumen fermentation affects ruminants productivity and the environmental impact of ruminant production. The release to the atmosphere of methane produced in the rumen is a loss of energy and a cause of climate change, and the profile of volatile fatty acids produced in the rumen affects the post-absorptive metabolism of the host animal. Rumen fermentation is shaped by intracellular and intercellular flows of metabolic hydrogen centered on the production, interspecies transfer, and incorporation of dihydrogen into competing pathways. Factors that affect the growth of methanogens and the rate of feed fermentation impact dihydrogen concentration in the rumen, which in turn controls the balance between pathways that produce and incorporate metabolic hydrogen, determining methane production and the profile of volatile fatty acids. A basic kinetic model of competition for dihydrogen is presented, and possibilities for intervention to redirect metabolic hydrogen from methanogenesis toward alternative useful electron sinks are discussed. The flows of metabolic hydrogen toward nutritionally beneficial sinks could be enhanced by adding to the rumen fermentation electron acceptors or direct fed microbials. It is proposed to screen hydrogenotrophs for dihydrogen thresholds and affinities, as well as identifying and studying microorganisms that produce and utilize intercellular electron carriers other than dihydrogen. These approaches can allow identifying potential microbial additives to compete with methanogens for metabolic hydrogen. The combination of adequate microbial additives or electron acceptors with inhibitors of methanogenesis can be effective approaches to decrease methane production and simultaneously redirect metabolic hydrogen toward end products of fermentation with a nutritional value for the host animal. The design of strategies to redirect metabolic hydrogen from methane to other sinks should be based on knowledge of the physicochemical control of rumen fermentation pathways. The application of new -omics techniques together with classical biochemistry methods and mechanistic modeling can lead to exciting developments in the understanding and manipulation of the flows of metabolic hydrogen in rumen fermentation.

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Fermentation of methanol in the sheep rumen

Cited by 50 publications

References 13 publications

Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat

Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat

Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions

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