Fermentation quality and microbial community of alfalfa and stylo silage mixed with Moringa oleifera leaves

Wang, Cheng; He, Liwen; Xing, Yaqi; Zhou, Wei; Fang, Yang; Chen, Xiaoyang; Zhang, Qing

doi:10.1016/j.biortech.2019.03.129

Cited by 110 publications

(90 citation statements)

References 36 publications

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“…Inoculation with L. plantarum had a positive effect on fermentation properties in terms of lower pH and higher LA accumulation. Similar effects were also reported by Wang et al (2019) and Yang et al (2019), in which LA fermentation was accelerated by L. plantarum inoculants in Moringa oleifera leaves and in alfalfa silage, respectively. The LPII exhibited more abundant LA fermentation than LPI.…”

Section: Effects Of L Plantarum On Fermentation Properties Of Alfalfsupporting

confidence: 85%

“…A series of Clostridia genera grew in the 90-d silage. Clostridia may lead to excessive protein degradation, DM loss, and butyric acid (BA) production in silage, and further promote the growth of other less acidtolerant spoilage microorganisms (Wang et al, 2019). Thus, they are undesirable in silage.…”

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Simentioning

confidence: 99%

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Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

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BackgroundThe objective of this study was to investigate the mechanism of Lactobacillus plantarum (L. plantarum) involved in improving fermentation quality of naturally ensiled alfalfa under poor conditions. ResultsHigh-moisture wilted alfalfa was ensiled without inoculants (CK) or with inoculation of two L. plantarum additives (LPI and LPII). The pH and fermentation products of silage were investigated, and the bacterial community compositions were analyzed. The L. plantarum inoculants significantly enhanced the lactic acid fermentation in terms of promotions in pH decline, lactic acid accumulation, and Lactobacillus abundance for both periods. At 90 d, silage in CK exhibited a high pH, a loss in dry matter, and a high concentration of ammoniacal nitrogen. The inoculations of L. plantarum significantly inhibited the growth of Clostridia, and reduced ammoniacal nitrogen concentration in silage (P < 0.05). Conclusions Inoculation with L. plantarum improved the fermentation quality of alfalfa silage and inhibited the growth of spoilage microorganisms, and further delayed decomposition of alfalfa silage under adverse ensiling conditions.

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Section: Effects Of L Plantarum On Fermentation Properties Of Alfalfsupporting

confidence: 85%

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Simentioning

confidence: 99%

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

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“…The NDF, ADF and CP contents of fresh IR were similar with the data reported by Yan et al (2019), whereas their DM and WSC contents were different. It was because the forage nutrition could be affected by climate, fertilization, irrigation and harvest time (Wang et al 2019a). The LAB counts on fresh IR was greater than 10 5 CFU per gram FW and sufficient for an adequate fermentation during ensiling (Wang et al 2017).…”

Section: Characteristics Of Fresh Italian Ryegrassmentioning

confidence: 99%

“…However, one of the main challenges during ensiling is the utilization of the wet material with uncontrolled fermentative processes, because several factors influence the synthesis of the fermentation products, such as the predominant micro-organisms in the silage, the fermentable substrates present and the types of fermentation (McDonald et al 1991). Nowadays, many researchers have evaluated the effects of microbial inoculants and mixed silages during ensiling and they showed substantial impacts on fermentation quality and microbial community (Forwood et al 2019;Wang et al 2019a;Wang et al 2019b). Nevertheless, there is a paucity of information regarding the fermentation type of silage.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, microbiota transplantation has become a good way to assess the effects of microbiota in many industries (Panke-Buisse et al 2015;Draper et al 2018). Next generation sequencing technology also provided more possibilities to accurately monitor the microbial succession during ensiling (Forwood et al 2019;Wang et al 2019a). As these research technologies developed, we first proposed to combine the cray irradiation, microbiota transplantation and next generation sequencing methods to elucidate the adaptation process of exogenous microbiota in silage.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of inoculating various epiphytic microbiota on fermentative profile and microbial community dynamics in sterile Italian ryegrass

et al. 2020

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Aims: To evaluate the effects of various epiphytic microbiota from Italian ryegrass (IR), maize (MZ) and sorghum (SG) on fermentative profile and microbial community dynamics in sterile IR. Methods and Results: Using microbiota transplantation, the irradiated IR was treated with the following: (i) sterile water; (ii) epiphytic microbiota on IR (IRIR); (iii) epiphytic microbiota on MZ (IRMZ); (iv) epiphytic microbiota on SG (IRSG). After 60 days of ensiling, MZ and SG microbiota significantly (P < 0Á05) decreased lactic acid (LA) and acetic acid (AA) concentrations compared to IR microbiota, while SG microbiota notably (P < 0Á05) reduced the ratio of LA to AA than MZ and IR microbiota. Apparently (P < 0Á01) higher amounts of Lactobacillus genus were observed in IRIR and IRMZ groups on 60 day compared to IRSG group, and the dominant Lactococcus genus on 3 day was eventually replaced by Enterobacteriaceae and Lactobacillus in IRSG group. Conclusions: Exogenous microbiota could evidently affect the fermentative profile and microbial community dynamics of IR silage. The numbers of Enterobacteriaceae and Lactobacillus were mainly responsible for this. Significance and Impact of the Study: Identifying the role of microbe during ensiling is of great significance to manipulate the fermentation products and improve the preservation of silage.

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Microorganisms that are critical for the fermentation quality of paper mulberry silage

Guo

Wang

Lin

et al. 2021

Food and Energy Security

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Silage quality remains an important issue in farming, as do limitations in the range of products suitable for animal fodder. We therefore explored the microorganisms that are critical for the fermentation quality of paper mulberry silage. Low (unwilted) and high (wilted) dry matter (DM) paper mulberry were harvested at two cutting times.These were ensiled for 0, 3, 7, 14, and 56 days, respectively. Compared with unwilted silages, wilting significantly decreased (p < 0.05) silage pH value, ammonia-N concentration, and yeast counts but increased (p < 0.05) lactic acid content. In addition, higher (p < 0.05) crude protein (CP) contents were also observed in wilted silages. Next-generation sequencing (NGS) analysis revealed that wilting reduced the abundance of Enterobacter, while increasing that of Lactobacillus. Single-molecule real-time sequencing (SMRT) revealed that the silage was enriched in the lactic acid bacteria (LAB), Lactobacillus rhamnosus after wilting, which showed a positive correlation with CP and lactic acid content. We conclude that wilting may help preserve paper mulberry silage, facilitating its use as a new fodder resource. Moreover, L. rhamnosus has the potential to be developed as a new inoculant for the modulation in wilted silages, particularly paper mulberry silage.

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Fermentation quality and microbial community of alfalfa and stylo silage mixed with Moringa oleifera leaves

Cited by 110 publications

References 36 publications

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Assessment of inoculating various epiphytic microbiota on fermentative profile and microbial community dynamics in sterile Italian ryegrass

Microorganisms that are critical for the fermentation quality of paper mulberry silage

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