Fermentative reforming of crude glycerol to 1,3-propanediol using Clostridium butyricum strain L4

Gupta, Pragya; Upreti, Manoj Kumar; Puri, S.K.; Ramakumar, S.S.V.

doi:10.1016/j.chemosphere.2021.133426

Cited by 21 publications

(7 citation statements)

References 36 publications

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“…While comparing PDO productivity, especially in fed‐batch fermentation of CG, most Clostridium strains, including C. butyricum L4, 2,3 VPI 1718 1 and DSP1 22 required >100 h of fermentation to produce a high titer of PDO (>70 g L −1 ). However, a few strains, such as C. butyricum AKR 102a 18 and DL07 28 needed ≈30 h of fermentation to support 76.2–94.2 g L −1 PDO with a productivity of 2–5.54 g/(L.h).…”

Section: Resultsmentioning

confidence: 99%

“…1,3-Propanediol (PDO) serves as a raw/building block material, specialty chemical, and additive for various preparations in several industries. [1][2][3][4][5] PDO can be produced from glycerol by anaerobic clostridial fermentation. [6][7][8] Through a reductive pathway, Clostridia produce PDO (C 3 H 8 O 2 ) from glycerol (C 3 H 8 O 3 ), 7,[9][10][11][12] while through an oxidative pathway they generate energy (ATPs), redox equivalents [e.g.…”

Section: Introductionmentioning

confidence: 99%

“…1,3‐Propanediol (PDO) serves as a raw/building block material, specialty chemical, and additive for various preparations in several industries 1‐5 . PDO can be produced from glycerol by anaerobic clostridial fermentation 6‐8 .…”

Section: Introductionmentioning

confidence: 99%

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High level of 1,3‐propanediol production from crude glycerol by Clostridium strain BOH3: critical roles of inoculum and substrate concentration

Rajagopalan,

Jain,

Poosarla

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUND1,3‐Propanediol (PDO) is a raw material/specialty chemical/additive in several industrial applications. A new wild‐type Clostridium strain BOH3 can utilize crude glycerol (CG) and produce PDO. This investigation reveals its potential for PDO production from CG.RESULTSInitially, inoculum of strain BOH3 was cultured from reinforced clostridial medium (RCM) with glucose (medium‐A), and modified‐RCM with glycerol (medium‐B). While fermenting 20 g/L glycerol, the cells cultivated from medium‐A produced 5.75 g/L PDO (as major product) with 3.10 g/L butanol (byproduct), whereas the cells cultivated from medium‐B produced 11.01 g/L PDO with 2.50 g/L butyric, and 1.01 g/L acetic acids, and no butanol was produced. To enhance PDO production from the batch‐process, various parameters including pure glycerol/CG concentration (80 g/L), inoculum age (15 h) and size (12.50%v/v) of cells from medium‐B, initial medium‐pH (pH 6.4), and incubation temperature (39 °C) were studied; the optimal values obtained are given in their respective parenthesis. Under optimal conditions, PDO production was 42.58–47.15 g/L (0.65–0.79 mol‐PDO/mol‐glycerol) from CG‐based rich/mineral media. Furthermore, fed‐batch fermentation with 50–80 g/L initial CG‐concentration was tested to enhance the PDO production. Among these, 60 g/L initial CG‐concentration assisted strain BOH3 in utilizing a high level of CG (~174 g/L); it supported a higher‐titer of PDO production (103.55–116.45 g/L), with the highest yield (0.72–0.81 mol‐PDO/mol‐glycerol) ever reported.CONCLUSIONClostridium strain BOH3 shows the potential to support a high titer (>116 g/L) with the highest yield (>0.80 mol/mol) of PDO production from CG (a waste). It can be considered for industrial‐scale PDO production.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High level of 1,3‐propanediol production from crude glycerol by Clostridium strain BOH3: critical roles of inoculum and substrate concentration

Rajagopalan,

Jain,

Poosarla

et al. 2024

J of Chemical Tech & Biotech

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“…This burgeoning trend propels the market for biobased products, prominently those featuring 1,3-propanediol (1,3-PDO). , This versatile biobased compound holds promise for diverse applications in various synthesis reactions. This bioproduct can be applied as a monomer in polycondensation processes, producing polyesters, polyether, and polyurethanes. , Beyond its pivotal role in polymerization, 1,3-PDO demonstrates utility across various applications, serving as a key component in solvents, adhesives, laminates, resins, detergents, and cosmetics. − …”

Section: Introductionmentioning

confidence: 99%

Salting-out as a Fast and Simple Method for First-Step Separation of Biobased 1,3-Propanediol

Liberato,

Buarque,

Ferreira

et al. 2024

J. Chem. Eng. Data

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The cost of 1,3-propanediol (1,3-PDO) microbial production is very much dependent on the separation of 1,3-PDO from the fermentation broth. The use of byproducts as nutrient sources has significant economic and environmental advantages. This specific molecule can be produced from the fermentation of raw glycerol. Crude glycerol is a primary byproduct in the biodiesel production process, and its large surplus blocks the development of the biodiesel industry. This study focused on separating 1,3-PDO through salting-out extraction (SOE), employing ethanol and 2propanol, along with five different salts, for screening. Subsequently, an experimental design was implemented to determine optimal alcohol and salt concentrations, maximizing key variables such as the partition coefficient and recovery yield. The entire quantity of 1,3-PDO was successfully recovered in the ethanolic phase, exhibiting a selectivity of 4.87 concerning glycerol. This achievement was realized by using 39 wt % KH 2 PO 4 and 19 wt % ethanol. According to the remarkable results, salting-out extraction based on ethanol and K 2 HPO 4 can be considered as an alternative and efficient platform for separating 1,3-PDO from glycerol, selectively obtaining two compounds with a high added value.

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“…In recent years, glycerol has been rapidly decreasing in price as biodiesel production has sharply increased . To increase profitability, glycerol is often further reacted into products with higher added value, including 1,3-propanediol, lactic acid, and glycerol carbonate (GC) . In particular, GC has a widespread application and a high price due to its low volatility, strong polarity, high boiling point, and environmental friendliness.…”

Section: Introductionmentioning

confidence: 99%

Process Design, Economic Optimization, and Environmental Assessment for the Production of Glycerol Carbonate via Reactive Distillation Combined with Pervaporation

Sun

Gao

Wei

et al. 2022

Ind. Eng. Chem. Res.

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In this study, a reactive distillation (RD) coupling pervaporation (PV) process was introduced to intensify the transesterification of dimethyl carbonate (DMC) with glycerol to produce glycerol carbonate. The yield of glycerol carbonate can be increased to more than 99.5% due to the timely removal of the by-product methanol. The PV module was used to recover DMC in the azeotrope (DMC/methanol) from the RD column. In addition, another RD process using pressure swing distillation (PSD) to recover DMC was simulated as a comparison. The design variables of both processes were optimized to obtain the lowest total annual cost (TAC), and the greenhouse gas emissions of the two processes were compared. Ultimately, in comparison to the RD-PSD process, the TAC of the RD-PV process was reduced by 18.67%, and the emissions of CO2, SO2, and NO x were reduced by 29.34, 29.52, and 29.52%, respectively.

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Fermentative reforming of crude glycerol to 1,3-propanediol using Clostridium butyricum strain L4

Cited by 21 publications

References 36 publications

High level of 1,3‐propanediol production from crude glycerol by Clostridium strain BOH3: critical roles of inoculum and substrate concentration

High level of 1,3‐propanediol production from crude glycerol by Clostridium strain BOH3: critical roles of inoculum and substrate concentration

Salting-out as a Fast and Simple Method for First-Step Separation of Biobased 1,3-Propanediol

Process Design, Economic Optimization, and Environmental Assessment for the Production of Glycerol Carbonate via Reactive Distillation Combined with Pervaporation

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