Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells

Sanchez-Villavicencio, Mayra Luz; Vinqvist‐Tymchuk, Melinda; Kalt, Wilhelmina; Matar, Chantal; Aguilar, Francisco J.; Villanueva, María del Carmen Escobar; Haddad, Pierre S.

doi:10.1186/s12906-016-1519-9

Cited by 25 publications

(14 citation statements)

References 26 publications

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“…Studies show that anthocyanins possess anti-obesity activity through the regulation of adipocytes, including induction of cell cycle arrest, reduction of lipid accumulation by suppressing transcription factors expressions such as PPARγ, C/EBPα, C/EBPβ, C/EBPδ, and aP2, and promoting lipolysis [ 9 ]. Interestingly, anthocyanin-enriched extracts from fermented blueberry juice, cranberry, blue pea flower ( Clitoria ternatea ), and blueberry peel inhibits adipogenesis and lipogenesis in 3T3-L1 adipocytes via downregulation of adipogenic genes [ 10 , 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin-Enriched Riceberry Rice Extract Inhibits Cell Proliferation and Adipogenesis in 3T3-L1 Preadipocytes by Downregulating Adipogenic Transcription Factors and Their Targeting Genes

et al. 2020

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Riceberry rice (Oryza sativa L.) is a new pigmented variety of rice from Thailand. Despite its high anthocyanin content, its effect on adipogenesis and adipocyte function remains unexplored. We investigated whether Riceberry rice extract (RBE) impacted cell proliferation by examining viability and cell cycle, using preadipocyte 3T3-L1 cells. To test RBE’s effect on adipocyte formation, cells were cultured in adipogenic medium supplemented with extract and adipocyte number and triglyceride levels were quantified. Furthermore, Akt1 phosphorylation along with RT-qPCR and intracellular calcium imaging were performed to obtain an insight into its mechanism of action. The effect of RBE on adipocyte function was investigated using glucose uptake and lipolysis assays. Treatment of cells with RBE decreased preadipocyte number without cytotoxicity despite inducing cell cycle arrest (p < 0.05). During adipogenic differentiation, RBE supplementation reduced adipocyte number and triglyceride accumulation by downregulating transcription factors (e.g., PPARγ, C/EBPα, and C/EBPβ) and their target genes (p < 0.05). The Akt1 phosphorylation was decreased by RBE but insignificance, however, the extract failed to increase intracellular calcium signals. Finally, the treatment of adipocytes with RBE reduced glucose uptake by downregulating Glut4 mRNA expression and enhanced isoproterenol-induced lipolysis (p < 0.05). These findings suggest that RBE could potentially be used in the treatment of obesity by inhibiting adipocyte formation and proliferation.

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Section: Introductionmentioning

confidence: 99%

Anthocyanin-Enriched Riceberry Rice Extract Inhibits Cell Proliferation and Adipogenesis in 3T3-L1 Preadipocytes by Downregulating Adipogenic Transcription Factors and Their Targeting Genes

et al. 2020

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“…Interestingly, several substrates identified in this study, such as Cdc42 and mTOR, are known to be involved in trophoblast migration and invasion (Knöfler & Pollheimer, ; Liu, Li, Na, & Liu, ). Vaccinium phytochemicals have been shown in other cell systems to affect the phosphorylation of AMPK (Eid et al, ; Sánchez‐Villavicencio et al, ), and we are the first to report that V . angustifolium crude leaf extract and chlorogenic acid increase AMPK phosphorylation in EVT cells.…”

Section: Discussionmentioning

confidence: 50%

“…All values are presented as mean ± standard error of the mean (n = 3). Data were analysed by one-way analysis of variance and Dunnett's multiple comparison test systems to affect the phosphorylation of AMPK (Eid et al, 2014;Sánchez-Villavicencio et al, 2017), and we are the first to report that V. angustifolium crude leaf extract and chlorogenic acid increase AMPK phosphorylation in EVT cells. We suspect the effects of these treatments seen on trophoblast migration and invasion occurred through an AMPK-dependent mechanism.…”

Section: Discussionmentioning

confidence: 99%

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

Ferrier

Gaudet

et al. 2018

Phytotherapy Research

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Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 10 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p < .01) and did not affect cell proliferation or viability. Chlorogenic acid was identified as a major phenolic compound of the leaf extract and the most active compound. Evidence from Western blot analysis (n = 3) suggests that the effects of the leaf extract and chlorogenic acid on trophoblast migration and invasion are mediated through an adenosine monophosphate-activated protein (AMP) kinase-dependent mechanism. Further investigations examining the potential therapeutic applications of this natural health product extract and its major chemical compounds in the context of placenta-mediated diseases are warranted.

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“…Heat dissipation and non-linear effects due to high frequencies, compressible systems (density as a function of the pressure drop), and others diseases which can affect the hearing system (hypercholesterolemia, hyperglycemia, genetic problems, etc.) lie outside of the scope of the present research [37][38][39][40][41]. A posible path to continue this research is to exted it to new models in the regime of nonlinear viscoelasticity (large deformations).…”

Section: Discussionmentioning

confidence: 86%

Electrorheological Model Based on Liquid Crystals Membranes with Applications to Outer Hair Cells

Herrera‐Valencia

Rey

2018

Fluids

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Liquid crystal flexoelectric actuation uses an imposed electric field to create membrane bending, this phenomenon is found in outer hair cells (OHC) located in the inner ear, whose role is to amplify sound through the generation of mechanical power. Oscillations in the OHC membranes create periodic viscoelastic flows in the contacting fluid media. A key objective of this work on flexoelectric actuation relevant to OHC is to find the relations and impact of the electro-mechanical properties of the membrane, the rheological properties of the viscoelastic media, and the frequency response of the generated mechanical power output. The model developed and used in this work is based on the integration of: (i) the flexoelectric membrane shape equation applied to a circular membrane attached to the inner surface of a circular capillary, and (ii) the coupled capillary flow of contacting viscoelastic phases, which are characterized by the Jeffreys constitutive equation with different material conditions. The membrane flexoelectric oscillations drive periodic viscoelastic capillary flows, as in OHCs. By applying the Fourier transform formalism to the governing equations and assuming small Mach numbers, analytical equations for the transfer function, associated to the average curvature, and for the volumetric rate flow as a function of the electrical field were found, and these equations can be expressed as a third-order differential equation which depends on the material properties of the system. When the inertial mechanisms are considered, the power spectrum shows several resonance peaks in the average membrane curvature and volumetric flow rate. When the inertia is neglected, the system follows a non-monotonic behavior in the power spectrum. This behavior is associated with the solvent contributions related to the retardation-Jeffreys mechanisms. The specific membrane-viscoelastic fluid properties that control the power response spectrum are identified. The present theory, model, and computations contribute to the evolving fundamental understanding of biological shape actuation through electromechanical couplings.

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Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells

Cited by 25 publications

References 26 publications

Anthocyanin-Enriched Riceberry Rice Extract Inhibits Cell Proliferation and Adipogenesis in 3T3-L1 Preadipocytes by Downregulating Adipogenic Transcription Factors and Their Targeting Genes

Anthocyanin-Enriched Riceberry Rice Extract Inhibits Cell Proliferation and Adipogenesis in 3T3-L1 Preadipocytes by Downregulating Adipogenic Transcription Factors and Their Targeting Genes

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

Electrorheological Model Based on Liquid Crystals Membranes with Applications to Outer Hair Cells

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