Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts

Abstract

In this study, Fourier transform infrared spectroscopy (FT‐IR), gel permeation chromatograph‐liquid chromatography (GPC‐LC), and scanning electron microscopy (SEM) were used to analyze the molecular characteristics of fermented Dendrobium officinale polysaccharides (FDOP) by Lactobacillus delbrueckii bulgaricus. The characteristic structural peak of FDOP was more prominent, showing a smaller molecular structure, and its porous structure showed better water solubility. The protective effect of FDOP on the damage of human skin fibroblasts (HSF) caused by ultraviolet (UV) radiation was investigated by evaluating its antioxidative and antiaging indices. The results showed that the antioxidant capacity of HSF was improved, and the breakdown of collagen, elastin, and hyaluronic acid was reduced, thus providing effective protection to the skin tissue. The antioxidative property of FDOP was explored using Nf‐E2‐related factor 2‐small interfering RNA‐3 (Nrf2‐siRNA‐3) (Nrf2‐si3) and qRT‐PCR (quantitative reverse transcription polymerase chain reaction), and the antiaging property of FDOP was explored using Western Blot and qRT‐PCR. The results show that FDOP can up‐regulate signal transduction of the Nrf2/Keap1 (Kelch‐like ECH‐associated protein 1) and transforming growth factor‐β (TGF‐β)/Smads pathways to reduce antioxidative damage and antiaging effects. Therefore, this study provides a theoretical basis for FDOP as a novel functional agent that can be used in the cosmetic industry.

Quantitative Identification of Antioxidant Basis for Dendrobium Nobile Flower by High Performance Liquid Chromatography-Tandem Mass Spectrometry

“…Some flavonoids, such as quercetin, rutin, and isoquercitrin, had been reported to be correlated antioxidant activities in D. officinale, D. catenatum, and D. huoshanense [12,[19][20][21]. Some polysaccharides were also considered as functional antioxidants in D. officinale, D. huoshanense, and D. nobile [15,22,23]. Recently, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) had been used for metabolic analysis, chemical differentiation, quality control, and pharmaceutical identification in some Dendrobium species [19,20,24].…”

The effects of alginate lyase treatment on structural characteristics and anti‐photoaging activity of alginate from Sargassum fusiforme

“…UV‐induced ROS activates the MAPK pathway, which subsequently activates activator protein‐1 (AP‐1); followed by upregulating MMP‐1, MMP‐3, and MMP‐9. MMPs could degrade the extracellular matrix, resulting in skin folds and inelasticity (Zhang et al., 2022). Therefore, the inhibition of the above MMPs could reflect the anti‐photoaging activity.…”

Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H2O2-Induced Oxidative Stress in Human Skin Fibroblasts

“…Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were assayed using the method in [ 19 ] as a reference.…”