Fermented Soy Permeate Reduces Cytokine Level and Oxidative Stress in Streptozotocin-Induced Diabetic Rats

Abstract

Oxidative stress and inflammation are involved in the development of type 1 diabetes and its complications. Because two compounds found in soy, that is, isoflavones and alpha-galactooligosaccharides, have been shown to exert antioxidant and anti-inflammatory effects, this study aimed to assess the effects of a dietary supplement containing these two active compounds, the fermented soy permeate (FSP). We hypothesized that FSP would be able to reduce in vivo oxidative stress and inflammation in streptozotocin (STZ)-induced type 1 diabetic rats. Thirty male Wistar rats were divided into the control placebo, diabetic placebo, and diabetic FSP-supplemented groups. They received daily, by oral gavage, water (placebo groups) or diluted FSP (0.1 g/day; FSP-supplemented group). After 3 weeks, glycemic regulation (glycemia and fructosamine level); the plasma level of carboxymethyllysine (CML), a marker of systemic oxidative stress in diabetes; and the plasma levels of inflammatory markers (CRP, IL-1β, IL-6, and uric acid) were evaluated. Markers of oxidative damage (isoprostanes and GSH/GSSG), antioxidant enzymatic activity (SOD and GPX), and Mn-SOD content were determined in skeletal muscle (gastrocnemius). Diabetic placebo rats exhibited higher CML levels, lower SOD and GPX activities, and decreased Mn-SOD contents. FSP supplementation in diabetic animals normalized the CML and antioxidant enzymatic activity levels and tended to increase Mn-SOD expression. The markers of inflammation whose levels were increased in the diabetic placebo group were markedly decreased by FSP (IL-1β: -75%, IL-6: -46%, and uric acid: -17%), except for CRP. Our results demonstrate that FSP exhibited antioxidant and anti-inflammatory properties in vivo in STZ-induced diabetic rats.

Investigation of the Acute Effects of Dry Extract of Glycine Max on Postprandial Glycemia in Rats

“…3A and 3B). Thus, in agreement with several studies that demonstrated antidiabetic properties to GM [7][8][9][10][11][12][13] we can conclude that isoflavones from dried extracts of GM show acute antihyperglycemic effect. The difference from our results and several studies showing antidiabetic properties for GM is the fact that those evaluations were done after chronic treatment and our evaluation involve acute effects of GM on post prandial glycemia elevation after an oral overload of soluble starch.…”

“…In agreement with this affirmation we previously demonstrated 6 activation of peroxisome proliferator-activated receptors α by using a methanolic fraction from soybean seeds rich in isoflavones (daidzin, glycitin, genistin, daidzein, malonylglycitin, malonylgenistin, genistein, glycitein, and malonyldaidzin). In addition, several studies reported antidiabetic properties of isoflavones from soy, not only in preclinical models 7,8 but also in humans 9,10 . The mechanisms by which isoflavones from soy produce antidiabetic effects include: antioxidant and anti-inflammatory properties 8 protection of beta cells 11 , and stimulation of insulin release 12 .…”

Sustained kidney biochemical derangement in treated experimental diabetes: a clue to metabolic memory

“…Decreased renal clearance of uric acid with resultant increases in the plasma or serum uric acid levels have been observed in rats after three or seven weeks of diabetes induced by streptozotocin4849. Therefore, we assessed the plasma and urine levels of uric acid (Supplemental Table 1) and found that the decreased renal clearance of uric acid in the hyperglycemic rats was not improved in the treated diabetic animals in both the short- and long-period groups (Fig.…”

Meta-analysis on the association between CYP2D6*10 gene polymorphism and disease free survival of breast cancer patients receiving tamoxifen treatment in Asia

“…15 studies were eliminated for duplicated publications. Finally full-text papers of 9 studies (Cai et al, 2014;Huang et al, 2013;Malarde et al, 2014;Mao et al, 2014;Saracoglu et al, 2012;Wang et al, 2014;Wang et al, 2013;Zhu et al, 2007) were searched from all the citations. A flow chart described the search method and study chose ( Figure 1).…”