Fermentor cultivation of Thermoplasma acidophilum for the production of cell mass and of the main phospholipid fraction

Freisleben, Hans-Joachim; Henkel, L.; Gutermann, Raymund; Rudolph, Petra; John, Gabriele; Sternberg, Brigitte; Winter, Stephen M.; Ring, Klaus

doi:10.1007/bf00173339

Cited by 14 publications

(23 citation statements)

References 15 publications

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“…44) In 1997, Swain et al identified -L-gulose as the sugar moiety of the MPL by MS, NMR, GLC-MS of acetylated butyl glycoside, and optical rotation data. 45) Thus the MPL was identified as -L-gulosylcaldarchaetidylglycerol.…”

Section: Polar Lipids From Thermoplasma Acidophilummentioning

confidence: 99%

Recent Advances in Structural Research on Ether Lipids from Archaea Including Comparative and Physiological Aspects

Koga

Morii

2005

Bioscience, Biotechnology, and Biochemistry

252

194

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Section: Polar Lipids From Thermoplasma Acidophilummentioning

confidence: 99%

Recent Advances in Structural Research on Ether Lipids from Archaea Including Comparative and Physiological Aspects

Koga

Morii

2005

Bioscience, Biotechnology, and Biochemistry

252

194

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“…Tetraether lipids have saturated carbon bonds which provide high oxidation resistance [22]. Due to these chemical properties high stability under extreme conditions such as low pH values [23] or high temperatures [24] can be acheived. Because of enhanced structural stability and eligibility of various protein modifications tetraether lipids are atractive as side-specific targeting agents in many biotechnological applications.…”

Section: Resultsmentioning

confidence: 99%

Selective interactions of concanavalin A‐modified tetraether lipid liposomes

Özcetin

Dayyoub

Hobler

et al. 2011

Phys. Status Solidi C

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For the development of site specific liposomes, high stability and long circulation properties are very promising for the improvement of drug targeting and drug delivery system as well as improvement of bioavailability of efficient bioactive drugs. Tetraether lipids (TELs) are very stabile lipids, extracted from Thermoplasma acidophilum. Because of its high chemical stability and its biocompatibility, Tetraether lipid liposomes prepared with TELs are excellent candidates for liposomal drug delivery. In this study a model protein, Concanavalin A (ConA) was chosen for a simulation of a specific adsorbtion onto mannan surface. Concanavalin A (ConA) is a lectin, extracted from Canavalia ensiformis, which interacts with sugar domains localized on inflammatory active tissues or tumors. In this study, stable TEL liposomes were prepared and characterized. Furthermore to develop side specific liposomes, tetraether lipids were activated and, ConA was covalently bond onto the surface of the prepared liposomes. Interaction of ConA conjugated liposomes with mannan‐modified surfaces was investigated by Biomolecular Interaction Analysis (BIA) via Reflectometric Interference Spectroscopy (RIfS). This provides real time observation of interactions between carbohydrate structure and TEL liposomes. Specific interaction was also visualised by AFM imaging subsequent to the RIfS measurements. According to the results, with ConA conjugated liposomes, high specific adsorption efficiency was observed. Consequently, this specific adsorption assay between ConA and mannan surface can be considered as a model for the further studies utilized with specific biomarkers for a selective active agent transfer to tumors and inflammatory active tissues. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…Previous reports regarding archaeal habitats have shown that the growth temperature of Thermoplasma species are well around 50 up to 60 o C (Huber et al 1991;Yasuda et al 1995) confirmed in fermentor growth (Freisleben et al 1994), whereas Sulfolobus species grow preferably at higher temperatures around 75-80 o C (Brock et al 1772). Hence, we took mud and water samples from five different mud holes and hot springs with temperatures above 50 o C. Samples were collected into 140 ml screw-capped glass bottles.…”

Section: Samplingmentioning

confidence: 86%

“…In Indonesia both, Sulfolobus and Thermoplasma species, which have not yet been further characterized, have been reported from Tangkuban Perahu (Huber et al 1991) an easily accessible volcano in West Java island, south of Jakarta, near the city of Bandung. Growth of Thermoplasma acidophilum was achieved in fermentors under laboratory conditions at pH 1.5 to 2.0 and an optimal growth temperature of 59 o C (Freisleben et al 1994), whereas Sulfolobus strains optimally grows around 75-80 o C and a pH between 2.5 and 3.5 (Brock et al 1972). Sulfolobus strains have become a major source of tetraether lipid, rather than Thermoplasma, although the latter lacks a cell wall, and thus the membrane lipids are 'naked' and easily accessible.…”

Section: Introductionmentioning

confidence: 99%

Archaeal Life on Tangkuban Perahu-Sampling and Culture Growth in Indonesian Laboratories

Handayani

Santoso

Freisleben

et al. 2012

HAYATI Journal of Biosciences

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The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 o C and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen's medium at 80 o C. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 o C in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt's medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.

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Fermentor cultivation of Thermoplasma acidophilum for the production of cell mass and of the main phospholipid fraction

Cited by 14 publications

References 15 publications

Recent Advances in Structural Research on Ether Lipids from Archaea Including Comparative and Physiological Aspects

Recent Advances in Structural Research on Ether Lipids from Archaea Including Comparative and Physiological Aspects

Selective interactions of concanavalin A‐modified tetraether lipid liposomes

Archaeal Life on Tangkuban Perahu-Sampling and Culture Growth in Indonesian Laboratories

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