Ferritin iron mobilisation by chelating agents

Comparison of the kinetics of iron release from a marine (Trichodesmium erythraeum) Dps protein and mammalian ferritin in the presence and absence of ligands

“…The removal of ca. 250 Fe/ HoSF in 5 h in the presence of 1 mM DFB is about twice as much as observed in a previous study [17], probably reflecting a higher Fe content per protein in our study.…”

“…Many studies of Fe release from storage proteins have focused on the role of chelating and reducing agents [17,18,27]. A few previous studies also suggest that small amount of Fe are released in the absence of added chelator.…”

“…The process involved in the dissolution of the Fe core in vivo remains unsolved, however. In vitro experiments show that Fe mobilization can occur in the presence of Fe(III) chelators or, more effectively, in the presence of reductants [17][18][19]. Whether reductants and chelators penetrate the protein shell has been a matter of some controversy.…”

Transfer of ferritin‐bound iron to adriamycin

“…It is well documented that ferritin iron can be released by iron chelators [16,17] and by reducing agents such as ascorbate, thiols and ~hydro~avins (reduced riboflavin, FMNHz, FMNHz) . It has also been demonstrated [23,24J, that ferritin in the presence of ascorbate can serve as source of iron in lipid peroxidation reactions.…”

Reduction/Dissolution of a β‐MnOOH Nanophase in the Ferritin Cavity To Yield a Highly Sensitive, Biologically Compatible Magnetic Resonance Imaging Agent

“…Alternatively, the absence of Mn II complexes of NTA or TETA (or of their oxidation products) may be explained in terms of their facilitated release from the inner compartment of the protein. In the case of iron ions, it has been reported that small negatively charged complexes can be released whereas Fe II aqua ions remain confined in the aqueous cavity [20,22,24,25] . Another explanation for the lack of Mn II complexes may rely on the complete oxidation of the polyaminocarboxylate molecule that has entered the cavity thus yielding to products characterized by being not longer coordinating agents.…”