Ferrocene Branched Chitosan for the Construction of a Reagentless Amperometric Hydrogen Peroxide Biosensor

Abstract

Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 x 10(-6) M and 2.0 x 10(-3) M. The biosensor reached 95% of the steady-state current in about 20 s and its sensitivity was 28.4 x 10(-3) microA x M(-1). The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 degrees C in 50 x 10(-3) M sodium phosphate buffer at pH 7.0.

One-step construction of biosensor based on chitosan–ionic liquid–horseradish peroxidase biocomposite formed by electrodeposition

“…HRP electrodes based on incorporation CS with IL, carbon nanotubes, nano Au particles and organically modified sol-gels were mainly compared. The detection limit obtained with the present method was lower than those obtained by HRP immobilization in CS-GD (Miao and Tan, 2000), CS-IC (Lu et al, 2006b), CS-Tb-MWCNT (Liu et al, 2008), CS-MWCNT (Qian and Yang, 2006), CS-nano Au (Lei et al, 2003(Lei et al, , 2004Tangkuaram et al, 2007), CS-THEOS sol-gel (Wang and Zhang, 2006) and FBCS (Garcia et al, 2007) systems, but higher than that obtained using HRP entrapping in CS-APDMOS sol-gel system (Wang et al, 2003). The response obtained with the present method was fast and only HRP electrode based on CS-APDMOS sol-gel system provided similar response rate (Wang et al, 2003).…”

Polyelectrostatic immobilization of gold nanoparticles-modified peroxidase on alginate-coated gold electrode for mediatorless biosensor construction

“…Different strategies has been described for connecting the catalytic active site of HRP with electrode surfaces, in order to construct such kind of third generation H 2 O 2 biosensors in which the direct electron transfer between the enzyme and the electrode is allowed without the use of any natural or artificial redox mediator. Among these methods, it should be highlighted the use of electroconductive polymers [2,3], metal nanoparticles [1,8], redox polymers and sol-gel materials [9][10][11], DNA [12] and carbon nanotubes [4] as wiring materials for HRP.…”

Chitosan‐Cross‐linked Osmium Polymer Composites as an Efficient Platform for Electrochemical Biosensors

“…7 The properties of chitosan can be tuned by the degree of deacetylation 8. The abundant amine groups of chitosan can be utilized for modifications, that is, for example, the attachment of redox centers, such as ferrocene, dopamine, and viologen for biosensing applications 9–15. Nanocomposites of chitosan with carbon nanotubes has been reported for the synergetic action on the biosensor response 16.…”