Ferrocene-Conjugatedm-Phenylenediamine Conducting Polymer-Incorporated Peroxidase Biosensors

Poly(phenylenediamine) film for the construction of glucose biosensors based on platinized glassy carbon electrode

“…The permeability and permselectivity of different polyphenylenediamine films for hydrogen peroxide, ascorbic acid, uric acid, acetaminophen, and cysteine have been reported [16]. PmPD films showed good permselectivity for hydrogen peroxide in the presence of [21][22][23][24][25]. It has been reported that the permselectivities of PPD polymers were concentration dependent [25].…”

“…Literature reports suggested that the performance of a glucose biosensor with electropolymerized coating varied greatly with fabricating conditions, including monomer structure, supporting electrolyte, pH, electropolymerization methods, glucose oxidase concentration, etc. [17][18][19][20][21][22][23][24][25][26] It has been reported that the interference blocking abilities and thus the permselectivities of PPD polymers were concentration dependent [25].…”

Organic phase enzyme electrodes

“…However, the electrode was more sensitive for tbutyl peroxide and lauroyl peroxide in aqueous medium. Despite the low solubility of peroxides in aqueous medium this should be expected since HRP conspicuously decreases its activity in organic medium (Mulchandani and Pan, 1999).…”

“…The electron transfer of the HRP could be direct or mediated. Mulchandani and Pan (1999) have developed an enzyme electrode based on horseradich peroxidase (HRP) incorporated in an electrically deposited ferrocene-modified phenylenediamine film on a glassy carbon electrode (GCE). The HRP/ poly(m-aminoanilinomethylferrocene)-modified GCE reagentless biosensor measured peroxides in both aqueous (citratephosphate buffer solution) and organic medium (90% acetonitrile-10% 2-(N-morpholino)ethanesulfonic acid buffer mixture) by reduction at a low applied potential of À0.05 V (versus Ag/AgCl) without interference from molecular oxygen.…”

Usage of Bismuth Film Electrode as Biosensor Transducer for Alkaline Phosphatase Assay

“…As mentioned above, it is possible to work with BiFE in the presence of oxygen which means BiFE can not detect oxygen change in the measuring solution. Electrochemical indication of oxidase-catalyzed enzymatic biosensors is possible by measuring either the consumption of oxygen or the oxidation of hydrogen peroxide [21]. In other words, these biosensors (that are called first generation biosensors) are dependent on the concentration of dissolved oxygen in the bulk solution [22].…”