Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay

Kanazawa, Masanori; Sato, Shinobu; Ohtsuka, Keiichi; Takenaka, Shigeori

doi:10.2116/analsci.23.1415

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Our work 1.0 Â 10 À9 to 1.0 Â 10 À7 3.2 Â 10 À10 Gold electrode 5 1.0 Â 10 À8 to 1.0 Â 10 À3 1.0 Â 10 À8 FRET-based probe 32 1.0 Â 10 À6 Electrochemical assay 33 2.0 Â 10 À7 to 1.0 Â 10 À5 2.0 Â 10 À7…”

Section: Methodsunclassified

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe₃O₄/multi-walled carbon nanotubes/SiO₂-surface molecular imprinting polymer

Duan

Wang

et al. 2015

RSC Adv.

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Section: Methodsunclassified

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe₃O₄/multi-walled carbon nanotubes/SiO₂-surface molecular imprinting polymer

Duan

Wang

et al. 2015

RSC Adv.

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“…Electrochemical methods, such as those involving ferrocenyl naphthalene diimide (FND) coupled to an mRNA-bound electrode, are used to detect RNase (Figure 8) [43]. FND has been developed as an electrochemical DNA duplex indicator [31,44], but FND also bound to mRNA with relatively low affinity, which could be due to the higher order structures containing double stranded regions within the mRNA.…”

Section: Rnase Detectionmentioning

confidence: 99%

Highly Sensitive Nuclease Assays Based on Chemically Modified DNA or RNA

Sato

Takenaka

2014

Sensors

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Nucleolytic enzymes are associated with various diseases, and several methods have been developed for their detection. DNase expression is modulated in such diseases as acute myocardial infarction, transient myocardial ischemia, oral cancer, stomach cancer, and malignant lymphoma, and DNase I is used in cystic fibroma therapy. RNase is used to treat mesothelial cancer because of its antiproliferative, cytotoxic, and antineoplastic activities. Angiogenin, an angiogenic factor, is a member of the RNase A family. Angiogenin inhibitors are being developed as anticancer drugs. In this review, we describe fluorometric and electrochemical techniques for detecting DNase and RNase in disease. Oligonucleotides having fluorescence resonance energy transfer (FRET)-causing chromophores are non-fluorescent by themselves, yet become fluorescent upon cleavage by DNase or RNase. These oligonucleotides serve as a powerful tool to detect activities of these enzymes and provide a basis for drug discovery. In electrochemical techniques, ferrocenyl oligonucleotides with or without a ribonucleoside unit are used for the detection of RNase or DNase. This technique has been used to monitor blood or serum samples in several diseases associated with DNase and RNase and is unaffected by interferents in these sample types.

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“…The use of electrochemical techniques is a highly promising approach to detecting RNase A, as it is user-friendly, cost-effective, and can be miniaturized. However, such a strategy has not been extensively investigated, and only our group [6] and Wangs group [7] have reported on it. The electrochemical method developed by our group was based on an electrode on which RNA is immobilized [6], with measurements performed in an electrolyte containing ferrocenylnaphthalene diimide (FND).…”

Section: Introductionmentioning

confidence: 99%

“…However, such a strategy has not been extensively investigated, and only our group [6] and Wangs group [7] have reported on it. The electrochemical method developed by our group was based on an electrode on which RNA is immobilized [6], with measurements performed in an electrolyte containing ferrocenylnaphthalene diimide (FND). The current signal from the FND decreased as a result of RNA cleavage by RNase A, and it was possible to detect the enzyme over the range of 0.2-10 ng mL À1 .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical RNase A Detection Using an Electrode with Immobilized Ferrocenyl Deoxyribooligonucleotide Containing Cytidine Residue

Sato

Takenaka

2013

Electroanalysis

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A ferrocenyl deoxyribooligonucleotide (FcODN(rC)) with contiguous cytosine bases and a single ribonucleotide, cytidine, was immobilized on a gold electrode, and this electrode was used to detect RNase A. RNase A activity in a solution was assessed using cyclic voltammetry, and it was found that the current response of the sensor electrode decreased with increasing enzyme concentration. An extremely low detection limit of 1.0 10 À11 g mL À1 RNase A was observed, with 15-90 % changes in the current signal. RNase activity can be an indicator of a number of diseases; therefore, this probe has great potential for applications in medical diagnostics.

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Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay

Cited by 5 publications

References 23 publications

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe₃O₄/multi-walled carbon nanotubes/SiO₂-surface molecular imprinting polymer

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe₃O₄/multi-walled carbon nanotubes/SiO₂-surface molecular imprinting polymer

Highly Sensitive Nuclease Assays Based on Chemically Modified DNA or RNA

Electrochemical RNase A Detection Using an Electrode with Immobilized Ferrocenyl Deoxyribooligonucleotide Containing Cytidine Residue

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Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay

Cited by 5 publications

References 23 publications

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe3O4/multi-walled carbon nanotubes/SiO2-surface molecular imprinting polymer

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe3O4/multi-walled carbon nanotubes/SiO2-surface molecular imprinting polymer

Highly Sensitive Nuclease Assays Based on Chemically Modified DNA or RNA

Electrochemical RNase A Detection Using an Electrode with Immobilized Ferrocenyl Deoxyribooligonucleotide Containing Cytidine Residue

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Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe₃O₄/multi-walled carbon nanotubes/SiO₂-surface molecular imprinting polymer

Biorecognition and highly sensitive determination of Ribonuclease A with chemiluminescence sensor based on Fe₃O₄/multi-walled carbon nanotubes/SiO₂-surface molecular imprinting polymer