Fertility after cancer: a prospective review of assisted reproductive outcome with banked semen specimens

Agarwal, Ashok; Ranganathan, Pavithra; Kattal, Namita; Pasqualotto, Fábio Firmbach; Hallak, Jaime Eduardo Cecílio; Khayal, Saba; Mascha, Edward J.

doi:10.1016/j.fertnstert.2003.07.021

Cited by 136 publications

(86 citation statements)

References 26 publications

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“…The findings of the present study may contribute to explaining the lower pregnancy rate when cryopreserved semen from cancer patients is used in IVF cycles (Kelleher et al 2001;Lass et al 2001;Ragni et al 2003;Agarwal et al 2004;Chung et al 2004;van Casteren et al 2008). Studies in animals and men demonstrate that DNA-fragmented spermatozoa can fertilise an oocyte and produce viable embryos (Ahmadi and Ng 1999;Fatehi et al 2006;Pérez-Cerezales et al 2010;Yamauchi et al 2012).…”

Section: Discussionmentioning

confidence: 61%

Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

Cambi

Marchiani

Manigrasso

et al. 2017

Reprod. Fertil. Dev.

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Abstract. Sperm cryopreservation is widely used by cancer patients undergoing chemo-or radiotherapy. Evidence suggests that IVF outcome with cryopreserved spermatozoa from cancer patients is less successful. To determine whether sperm DNA fragmentation (SDF) is involved in the lower fertilising ability of cryopreserved spermatozoa of cancer patients, SDF was evaluated in thawed spermatozoa from 78 men affected by different cancers and 53 men with noncancer pathologies. SDF was assessed by the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL), propidium iodide (PI), flow cytometry procedure, which allows determination of two different cell populations (PI brighter and PI dimmer ) and thus to determine the percentage of DNA fragmented sperm in both. PI dimmer spermatozoa are totally unviable, whereas PI brighter spermatozoa with SDF may be motile and morphologically normal, having higher biological relevance in the reproductive process. We found that the proportion of DNA fragmented PI brighter cells was significantly higher in thawed spermatozoa from cancer than non-cancer patients. Moreover, a positive correlation was found between the degree of DNA fragmentation and sperm motility in the PIbrighter population of spermatozoa from cancer patients that wasn't seen in non-cancer patients. The results of the present study suggest that higher SDF levels may contribute to the lower IVF success of cryopreserved spermatozoa from cancer patients and that evaluation of SDF could complement genetic counselling as part of the routine management of cancer patients who seek fertility preservation.Additional keywords: cancer patients, flow cytometry, propidium iodide, terminal deoxyribonucleotidyl transferasemediated dUTP-digoxigenin nick end-labelling (TUNEL).

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Section: Discussionmentioning

confidence: 61%

Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

Cambi

Marchiani

Manigrasso

et al. 2017

Reprod. Fertil. Dev.

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“…These data are more successful than those quoted in other studies involving men treated for cancer (Table 4). [15][16][17][18][19] However, most of the latter were reported around 10 years before the current study and our data are likely to reflect improvements in reproductive techniques over the last decade.…”

Section: Discussionmentioning

confidence: 70%

Uptake and outcome of assisted reproductive techniques in long-term survivors of SCT

Babb

Farah

Lyons

et al. 2011

Bone Marrow Transplant

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We have audited the invitation for uptake and outcome of artificial reproductive techniques in patients undergoing SCT for haematological malignancy, with the aim of improving our pre-transplant counselling. A postal survey was sent to 434 patients in our centre surviving a minimum of 2 years after allo-SCT, of whom 221 patients responded. Of 112 male patients, 79 were offered sperm storage, 42 banked sperm and 25 subsequently attempted parenthood with stored sperm. A total of 18 were successful, with 29 children born a median of 8 years (range 1-22 years) following SCT. Of 72 females o42 years old, 33 were offered storage of embryos/eggs/ ovarian tissue and 12 accepted. Following SCT, four women attempted pregnancy using cryopreserved embryos, with two successes. The majority of patients who were not counselled about infertility or not offered fertility-preservation options provided a likely reason, with completion of family being the most frequent. Nonetheless, 16 patients (11/72 women and 5/112 men) could not provide a reason for the lack of information/ invitation. In conclusion, uptake of gamete/embryo storage is high when offered and collected material is used frequently. Pregnancies in partners of male patients were usually successful and our data highlight the value of prolonged cryostorage.

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“…[12][13][14][15][16][17] As ART continues to improve, success rates from older studies are not helpful in counselling patients. Patients need up-to-date numbers in order to make informed choices.…”

Section: Discussionmentioning

confidence: 99%

Use of an oncology sperm bank: a Canadian experience

Selk

Belej-Rak

Shapiro

et al. 2013

CUAJ

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Fertility after cancer: a prospective review of assisted reproductive outcome with banked semen specimens

Cited by 136 publications

References 26 publications

Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

Uptake and outcome of assisted reproductive techniques in long-term survivors of SCT

Use of an oncology sperm bank: a Canadian experience

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